LOCUS       sequence-386791-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable and dox. inducible expression of Trim69 isoform E (codon 
            optimized) after retroviral-mediated gene transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Song Y, Nguyen XN, Kumar A, da Silva C, Picard L, Etienne L, 
            Cimarelli A
  TITLE     Trim69 is a microtubule regulator that acts as a pantropic viral 
            inhibitor.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 
            10.1073/pnas.2211467119. Epub 2022 Oct 17.
  PUBMED    36251989
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2211467119. 
            doi"; pages: " 10.1073/pnas.2211467119. Epub 2022 Oct 17"
COMMENT     Sequence Label: pRetroX-Tight-Pur-Trim69 isoform E (codon opti...
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgataagggt ggcggagcgg ccgccgagga ggagctggcc
       61 attcagcagg gccagctgga gaccaccctg aaggagctgc aaaccctcag gaacatgcag
      121 aaggaggcca tcgccgctca caaggatatt accaccctgc tgcactccct ggagcagggc
      181 atgaaggtcc tggccaccag agagctcatt tccaggaagc tgaacctggg ccagtataag
      241 ggccccattc agtacatggt gtggagggag atgcaggaca ccctgtgtcc cggcctgagc
      301 cctctgacac tggaccccaa aacagcccac cccaatctgg tgctgtccaa gagccagacc
      361 tccgtgtggc atggcgacat caagaagatc atgcccgatg accccgagag gtttgacagc
      421 agcgtcgctg tgctgggctc cagaggcttt accagcggca aatggtactg ggaagtggag
      481 gtggccaaaa agaccaaatg gacagtggga gtggtgaggg aaagcattat caggaagggc
      541 agctgtcctc tgacacctga gcagggcttt tggctgctga ggctgagaaa ccagacagac
      601 ctgaaggccc tggacctccc cagcttcagc ctgaccctga caaacaacct ggacaaggtc
      661 ggcatctacc tggactacga gggaggccag ctgagcttct ataatgccaa aaccatgaca
      721 cacatctata ccttttccaa cacatttatg gagaagctgt acccttattt ctgtccctgc
      781 ctgaacgacg gcggcgaaaa taaggagccc ctgcacattc tgcaccccca gtga
//
