LOCUS       sequence-386797-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of eTd-CBEm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Chen L, Zhu B, Ru G, Meng H, Yan Y, Hong M, Zhang D, Luan C, Zhang 
            S, Wu H, Gao H, Bai S, Li C, Ding R, Xue N, Lei Z, Chen Y, Guan Y, 
            Siwko S, Cheng Y, Song G, Wang L, Yi C, Liu M, Li D
  TITLE     Re-engineering the adenine deaminase TadA-8e for efficient and 
            specific CRISPR-based cytosine base editing.
  JOURNAL   Nat Biotechnol. 2022 Nov 10. doi: 10.1038/s41587-022-01532-7.
  PUBMED    36357717
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 10. doi: 10.1038/s41587-022-01532-7."
COMMENT     Sequence Label: eTd-CBEm
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..360
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     311..331
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     402..421
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        402..420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             470..490
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(989..1012,1013..1015,1016..5089)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             989..5089
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5132..5152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5162..5218
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5219..5467
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5498..5746
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5789..5809
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5818..5835
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5858..5875)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5864..6088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6159..6175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6159..6175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6172..6194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6183..6199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6207..6213,6214..6231,6232..6237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6252..6273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6390..6407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6561..7149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6641..6660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7320..8111,8112..8180))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7943..7962
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8181..8285)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8364..8383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8555..8778,1..156)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
       61 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      121 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      181 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      241 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      301 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      361 ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg
      421 gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac caaagaagaa
      481 gcggaaagtc tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct
      541 ggccaagagg gcacgggatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa
      601 tagagtgatc ggcgagggct ggctgagaat gatcggcctg cacgacccaa cagcccatgc
      661 cgaaattatg gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc
      721 caccctgtac gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag
      781 gatcggccgc gtggtgtttg gcgtgaggaa ctcaaaaaga ggcgccgcag gctccctgat
      841 gaacgtgctg aactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc
      901 agatgaatgt gccgccctgc tgtgcgattt ctatcggatg cctagacagg tgttcaatgc
      961 tcagaagaag gcccagagct ccatcaacga caagaagtac agcatcggcc tggccatcgg
     1021 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     1081 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     1141 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     1201 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     1261 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     1321 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     1381 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     1441 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     1501 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     1561 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     1621 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     1681 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     1741 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     1801 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     1861 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     1921 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     1981 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     2041 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     2101 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     2161 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     2221 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     2281 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     2341 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     2401 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     2461 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     2521 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     2581 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     2641 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     2701 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     2761 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     2821 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     2881 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     2941 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     3001 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     3061 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     3121 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     3181 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     3241 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     3301 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     3361 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     3421 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     3481 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     3541 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     3601 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     3661 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     3721 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     3781 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     3841 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     3901 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     3961 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     4021 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     4081 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     4141 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     4201 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     4261 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     4321 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     4381 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     4441 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     4501 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     4561 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     4621 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     4681 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     4741 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     4801 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     4861 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     4921 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     4981 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     5041 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggtgact ctggcggctc
     5101 aaaaagaacc gccgacggca gcgaattcga gcccaagaag aagaggaaag tcggaagcgg
     5161 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctac
     5221 taatctgagc gacatcattg agaaggagac tgggaaacag ctggtcattc aggagtccat
     5281 cctgatgctg cctgaggagg tggaggaagt gatcggcaac aagccagagt ctgacatcct
     5341 ggtgcacacc gcctacgacg agtccacaga tgagaatgtg atgctgctga cctctgacgc
     5401 ccccgagtat aagccttggg ccctggtcat ccaggattct aacggcgaga ataagatcaa
     5461 gatgctgagc ggaggatccg gaggatctgg aggcagcacc aacctgtctg acatcatcga
     5521 gaaggagaca ggcaagcagc tggtcatcca ggagagcatc ctgatgctgc ccgaagaagt
     5581 cgaagaagtg atcggaaaca agcctgagag cgatatcctg gtccataccg cctacgacga
     5641 gagtaccgac gaaaatgtga tgctgctgac atccgacgcc ccagagtata agccctgggc
     5701 tctggtcatc caggattcca acggagagaa caaaatcaaa atgctgtctg gcggctcaaa
     5761 aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat
     5821 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5881 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5941 actgttcttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6001 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6061 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     6121 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6241 tagggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6301 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6361 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6961 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7141 ggatctcaag aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac
     7201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7621 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7681 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7741 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7801 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7861 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7921 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7981 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8041 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8101 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8161 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8281 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc
     8341 gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8401 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8461 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8521 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     8581 tagtaatcaa ttacggggtc attagttcat agcccatata ttgagttccg cgttacataa
     8641 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     8701 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     8761 tatttacggt aaactgcc
//
