LOCUS       sequence-386819-        2534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains the coding sequence for signaling peptide M12,
            which contains the RPTAVEG signaling motif. Digest this insert and 
            add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W,
            Bianco S, Lim WA
  TITLE     Decoding CAR T cell phenotype using combinatorial signaling motif 
            libraries and machine learning.
  JOURNAL   Science. 2022 Dec 16;378(6625):1194-1200. doi: 
            10.1126/science.abq0225. Epub 2022 Dec 8.
  PUBMED    36480602
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abq0225"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1194-1200"
COMMENT     Sequence Label: pMAT_M12_RPTAVEG
FEATURES             Location/Qualifiers
     source          1..2534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(677..694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(787..1375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(867..886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1546..2337,2338..2406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2169..2188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2407..2511)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccacg gtacccgggg atccacctgc aacactccgg
      421 atcaggatct ggatctggcc ctggctctag acctacagct gtggaaggac tggctctggg
      481 cagttctggc tccggatcag gatccggcag tggtacaagg caggtggatc ctctagagtc
      541 gttttaatta atggagcaca agactggcct catgggcctt ccgctcactg cccgctttcc
      601 agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc cttgcgtatt
      661 gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg
      721 gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      781 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      841 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      961 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1081 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1141 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1201 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1261 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1321 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1381 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1441 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1501 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1561 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1621 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1681 accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1741 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1801 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1861 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1921 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1981 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2041 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2101 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2161 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2221 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2281 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2341 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2401 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2461 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2521 ccgaaaagtg ccac
//
