LOCUS       sequence-386822-        2525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains the coding sequence for signaling peptide M3, 
            which contains the YENL signaling motif. Digest this insert and add 
            it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W,
            Bianco S, Lim WA
  TITLE     Decoding CAR T cell phenotype using combinatorial signaling motif 
            libraries and machine learning.
  JOURNAL   Science. 2022 Dec 16;378(6625):1194-1200. doi: 
            10.1126/science.abq0225. Epub 2022 Dec 8.
  PUBMED    36480602
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abq0225"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1194-1200"
COMMENT     Sequence Label: pMAT_M3_YENL
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(668..685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(778..1366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(858..877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1537..2328,2329..2397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2160..2179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2398..2502)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccacg gtacccgggg atccacctgc aacactccgg
      421 atcaggatct gaagaaggcg cccctgacta cgagaatctg caagagctga atcaccccgg
      481 ctccggatca ggatccggca gtggtacaag gcaggtggat cctctagagt cgttttaatt
      541 aatggagcac aagactggcc tcatgggcct tccgctcact gcccgctttc cagtcgggaa
      601 acctgtcgtg ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct
      661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta
      721 atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      781 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      841 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      901 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      961 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1021 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1081 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1141 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1201 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1261 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1321 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1381 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1441 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1501 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1561 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1621 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1681 accacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1741 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1801 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1861 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1921 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1981 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2041 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2101 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2161 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2221 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2281 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2341 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2401 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2461 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2521 gccac
//
