LOCUS       sequence-386835-        2566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains the coding sequence for the intracellular 
            domain of CD28. Digest this insert and add it to 
            pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W,
            Bianco S, Lim WA
  TITLE     Decoding CAR T cell phenotype using combinatorial signaling motif 
            libraries and machine learning.
  JOURNAL   Science. 2022 Dec 16;378(6625):1194-1200. doi: 
            10.1126/science.abq0225. Epub 2022 Dec 8.
  PUBMED    36480602
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abq0225"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1194-1200"
COMMENT     Sequence Label: pMAT_CD28ICD
FEATURES             Location/Qualifiers
     source          1..2566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(709..726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(819..1407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(899..918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1578..2369,2370..2438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2201..2220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2439..2543)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatc ggtacccggg gatccacctg caacactccg gatcaggatc ttgggtccga
      421 agcaagagaa gcagactgct gcacagcgac tacatgaaca tgacccctag acggcccgga
      481 cctaccagaa agcactacca gccttacgct cctcctagag acttcgccgc ctacagatct
      541 ggctccggat caggatccgg cagtggtaca aggcaggtgg atcctctaga gtcgctgggc
      601 ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      661 ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct cgctcactga
      721 ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa
      781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca
     1741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
