LOCUS       sequence-386838-        2531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains the coding sequence for signaling peptide M10,
            which contains the PQQAT signaling motif. Digest this insert and add
            it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W,
            Bianco S, Lim WA
  TITLE     Decoding CAR T cell phenotype using combinatorial signaling motif 
            libraries and machine learning.
  JOURNAL   Science. 2022 Dec 16;378(6625):1194-1200. doi: 
            10.1126/science.abq0225. Epub 2022 Dec 8.
  PUBMED    36480602
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abq0225"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1194-1200"
COMMENT     Sequence Label: pMAT_M10_PQQAT
FEATURES             Location/Qualifiers
     source          1..2531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(674..691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(784..1372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(864..883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1543..2334,2335..2403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2166..2185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2404..2508)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccacg gtacccgggg atccacctgc aacactccgg
      421 atcaggatct gacgatagcc tgcctcatcc tcagcaggcc acagatgata gcggccacga
      481 atctggctcc ggatcaggat ccggcagtgg tacaaggcag gtggatcctc tagagtcgtt
      541 ttaattaatg gagcacaaga ctggcctcat gggccttccg ctcactgccc gctttccagt
      601 cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg
      661 cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg
      721 tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1561 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1621 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1681 gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1741 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1801 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1861 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1921 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1981 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2041 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2101 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2161 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2221 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2281 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2341 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2401 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2461 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2521 aaaagtgcca c
//
