LOCUS       sequence-38685-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Cowley SC, Av-Gay Y
  TITLE     Monitoring promoter activity and protein localization in 
            Mycobacterium spp. using green fluorescent protein.
  JOURNAL   Gene. 2001 Feb 21;264(2):225-31.
  PUBMED    11250077
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-02-21"; volume: "264(2)"; pages: "225-31"
COMMENT     Sequence Label: pSC300
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..85)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(296..312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(296..312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(309..331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    320..336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(344..350,351..368,369..374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    389..410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(527..544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(778..797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcctttac tcattttttc taccggtaca gcttgatatc gaattcctgc agcccggggg
       61 atcccacggc attccttcct tcgatgaaat gatcgtccga tgtttctttt cgcgcgggtg
      121 cctcccacgc gatgggctgg ctctagcttg acaagtcaac cctgctccat gaccgctcgc
      181 gtcgcaacaa cgaccttggg gtcgatgccc cagggcggac ggcataggct accaggacgc
      241 ccccaggctg tctagagcgg ccgccaccgc ggtgcgagcc agatctggta atcatgtcat
      301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      841 ttaccggata
//
