LOCUS       sequence-386867-        8843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW1KN0034
FEATURES             Location/Qualifiers
     source          1..8843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      315..509
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(745..767)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    853..993
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     908..927
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1008..1025)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1179..1767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1259..1278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1855..2670)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1855..2670)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2578..2597
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    3103..3127
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3231..3576
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3519..3539
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3667..5316
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3733..3751)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             5380..5403
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5441..5615
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5636..5660
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6961..7590
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(8019..8843,1..249)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
       61 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
      121 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
      181 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
      241 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
      301 aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
      361 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
      421 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
      481 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagtgctg
      541 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
      601 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
      661 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
      721 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga cggtcacagc
      781 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      841 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
      901 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
      961 cacagatgcg taaggagaaa ataccgcatc aggcgctcat ccgcttcctc gctcactgac
     1021 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1081 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1141 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1741 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1801 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtgattagaa
     1861 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     1921 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     1981 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     2041 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     2101 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     2161 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     2221 agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     2281 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     2341 taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg
     2401 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     2461 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     2521 tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc
     2581 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga
     2641 gcaagacgtt tcccgttgaa tatggctcat aacagaactt attatttcct tcctcttttc
     2701 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
     2761 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
     2821 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
     2881 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     2941 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     3001 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     3061 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     3121 tgtaaacata acgaattgct cttcaatgag gaggtcaaca tggtggagca cgacactctg
     3181 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
     3241 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     3301 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     3361 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
     3421 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
     3481 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     3541 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
     3601 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
     3661 gatacaatgg aggacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
     3721 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
     3781 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
     3841 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
     3901 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
     3961 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
     4021 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
     4081 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
     4141 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
     4201 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
     4261 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
     4321 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
     4381 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
     4441 ggatttcgag tcgtcttaat gtatagattt gaagaagaac tgtttctgag gagccttcag
     4501 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc
     4561 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc
     4621 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa
     4681 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     4741 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     4801 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg
     4861 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     4921 cattctggag acatagctta ctgggacgag gacgaacact tcttcatcgt tgaccgcctg
     4981 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg
     5041 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc tgcccgacga tgacgccggt
     5101 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     5161 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     5221 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     5281 ctcataaagg ccaagaaggg cggaaagatc gccgtgggtt cggattataa ggaccatgac
     5341 ggagactata aggaccatga cctcgacgct gcagcagcgg attataagga cgatgacgat
     5401 aagtgagctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     5461 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     5521 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     5581 tcctaaaacc aaaatccagt actaaaatcc agatcgctag catgaagagc aattctgaca
     5641 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     5701 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     5761 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     5821 gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     5881 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     5941 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     6001 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     6061 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     6121 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     6181 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     6241 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     6301 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     6361 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     6421 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     6481 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     6541 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     6601 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     6661 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     6721 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca
     6781 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     6841 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     6901 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     6961 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     7021 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     7081 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     7141 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     7201 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     7261 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     7321 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     7381 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     7441 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     7501 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     7561 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     7621 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     7681 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     7741 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     7801 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     7861 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     7921 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     7981 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     8041 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     8101 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     8161 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     8221 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     8281 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     8341 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     8401 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     8461 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     8521 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     8581 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     8641 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     8701 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     8761 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     8821 actttttgat cgatcccggc atc
//
