LOCUS       sequence-386870-        3390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: AtARF18 promoter 1kb upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0018
FEATURES             Location/Qualifiers
     source          1..3390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(31..125)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(146..805)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     720..739
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     967..985
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(989..1032)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1035..1056
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2398..2455
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2479..2496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2650..3238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2730..2749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
       61 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      121 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg cagtactgtt
      181 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      241 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      301 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      361 gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      421 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      481 attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      541 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      601 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      661 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      721 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      781 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      841 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
      901 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
      961 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     1021 aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc gggaggaaaa
     1081 aaaagaatac agcatgaaca ccagcgactc agaactgact cggcacgtgg taacataaaa
     1141 ctcacacgct tcccaagacg ctcgtcccat aaagagtcat ggctgtgacc ttgtgcgagt
     1201 gtgggtgtag ccatagcctt tgtgggtcct aaataaaaaa aacgatcaat ggcataatgt
     1261 gtaattaact cacattccac agtgttaaat aaaaattaaa tctgtaacgg aaaaaacaat
     1321 ctatctgatg ttacacaaaa aaaaaaaata acggaaaaaa gaaaatgtgg ccggggttta
     1381 atagagaagc cgacacgttt gcctgccacg atccagacac aagacgatgt gggtaacggc
     1441 accgtcaagt gctaacggca acggggtcat cagtaaaaag ggcaatttag ggatatagag
     1501 taaagtcata agcttattta ttactatcgt cttgatcgga cggctgagat ttgccctctc
     1561 ctttaaatgc accgtacgtt ttttttttac ataataaaat gtaattagtt cataagcaat
     1621 tcccaaaacg ccgcgtgacc cttcgtttgc ctttgaatac aatttgtttt attttagcgc
     1681 atttaatata tttgagtttc ctttttagcg atgacatgat aaaacaaaaa acaacaattt
     1741 tagaaaacat ttaattaaat gaatagaaaa atagggagat tcaactgaag ttactgtggg
     1801 tggaaatgac aggtcaccag gatctgaact aagagaaagt ctctctatat tttgatatat
     1861 agagagagat aaagaaggag agagtgtgaa tatatgtatg tgcatgatac tgtgagactt
     1921 cttcagtaaa ggaagcagct ttggtttgct gcggatcggg ataaaaacgg cgagaagttt
     1981 gtgattgttg aagcaaaaca acacggaatc tgaaaggaaa ggagaagaca aaaaaaaaaa
     2041 aagattttaa gaggaagaga gagagagatc ggtttcgtgg ctgacggaaa aaaaaagggg
     2101 agagatttag ttttatccaa cagtgcctga attacgttct gcaatcaata cggacataac
     2161 cgtccgttgt gtcctgttta taaagttttg ctttttttat tttctccatt gatgggtctt
     2221 ttctttcttc tctctctcgt gtttctttca tggggttaag actagtgttt accgcgtgaa
     2281 ggtttttttt tctttctatt ttctttcatt tcctctcctt ctacttcttc ttctccagtt
     2341 ctcatctggg ttcttcaatg tgagaccact catcgcgact agtagcggcc gctgcagtcc
     2401 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     2461 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2641 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3061 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3301 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3361 aagttttaaa tcaatctaaa gtatatatga
//
