LOCUS       sequence-386871-        3136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: ANAC032 promoter 1kb upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0014
FEATURES             Location/Qualifiers
     source          1..3136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(114..773)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     688..707
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     935..953
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(957..1000)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2112..2169
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2193..2210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2364..2952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2444..2463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(3135..3136,1..93))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
       61 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
      121 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      181 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      241 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      301 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
      361 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      421 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      481 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      541 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      601 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      661 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      721 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      781 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      841 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
      901 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
      961 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagt
     1021 tgagcttttt atcaaattca acccgccaca ataaaaagcg gcagaactaa cattccggct
     1081 ccacgtggca tagatcgaag cgtttatcta ttaggtggca caggcggctc gagatgacgt
     1141 tcttgtggac tccattagag aaaatgacag aattaagtgg gcattagcat aagttgataa
     1201 ctatgaggtg ctttttaaaa agaaaactaa cagctgcgat tctgtttttt tttgccgtta
     1261 gtgtacgcta aatcatagac ctcttcgtgg ttataaagaa agaaacttaa tttgtctcga
     1321 atttgagttt ggatgcgacg ttttacgtac tagttttata aaagtaaatg ttatagacct
     1381 tgaatttcaa agtggaatgt attttacaca cacaattgat acaccaacaa ttgtggaagc
     1441 aaggatatag agtatcgatc tttaatggag aagaatatat ccttaaagta atcattactt
     1501 tttaattacg gcggaaagag aatagttaaa tttacggcga aaagaatgtt cattaatcaa
     1561 ttgatgacgt tcgtttataa tttcaaataa gatcgaaaat aacaggtgaa caaagaaaaa
     1621 atagacatta aaaatagtaa gatcgaaaat acaaccctta aaaatctcac gaaatcaatg
     1681 agagaaacaa ttttgagata cggtcaacgt ttggtcacct ttaggaaagt acacgcataa
     1741 gatcgaacgt gtcgattctt attgctcgtt cccaaaatcg acatgctctc ggaccgctac
     1801 attccaaata gtctgacgta agcaatgaca aaactcacct acattacagt ctgccacgtg
     1861 tccctctcca tctacttcaa atgaatcctc cactatacag atttaaccat ttttctattt
     1921 ttgtttgaga ttcctttcca ccaacacaaa gcccctgtct atccgccatg tgtccacgac
     1981 ctttcttata tatatagtca tctttcttct ttgctcgaga gaagtttcaa ctttcaaaca
     2041 tatctttaca gttctttctt gctaaacaac aataaagggg aaaataggtt aattaatgcg
     2101 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     2161 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     2221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2341 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     2401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3121 agctcgaggc ttggat
//
