LOCUS       sequence-386873-        2161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: MinSyn_311.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYC0CM0529
FEATURES             Location/Qualifiers
     source          1..2161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(633..727)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(748..1407)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1322..1341
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1569..1587
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1591..1634)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1771..1828
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1852..1869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2023..2161,1..450))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2103..2122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
       61 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      121 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      181 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      241 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      301 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      361 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      421 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      481 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      541 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      601 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
      661 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      721 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
      781 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
      841 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
      901 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
      961 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1021 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1081 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1141 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1201 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     1261 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     1321 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     1381 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     1441 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     1501 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     1561 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     1621 ctaaggatga tttctggaat tcggtctcag gagggaggcg tgtcgtttta gtgaggctta
     1681 cgtctgggat tacgtctact atataaggtt ttgctattca ttgaaagcag tagtgactga
     1741 tttgtatata atgtactcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc
     1801 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
     1861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
     2041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2161 g
//
