LOCUS       sequence-386877-        2161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: MinSyn_310.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYC0CM0528
FEATURES             Location/Qualifiers
     source          1..2161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(620..714)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(735..1394)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1309..1328
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1556..1574
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1578..1621)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1758..1815
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1839..1856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2010..2161,1..437))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2090..2109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
       61 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
      601 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      661 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      721 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      781 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      841 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      901 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
      961 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1021 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1081 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1141 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1201 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1261 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1321 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1381 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1441 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac
     1501 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1561 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     1621 ctggaattcg gtctcaggag ggaggcgtgt cgttttagtg aggctgacgt aagggatgac
     1681 gtaaactata taaggttttg ctattcattg aaagcagtag tgactgattt gtatataatg
     1741 tactcgagac cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
     1801 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
     1861 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1981 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
     2041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2161 t
//
