LOCUS       sequence-386878-        2205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: MinSyn_301.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYC0CM0515
FEATURES             Location/Qualifiers
     source          1..2205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     204..223
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     451..469
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(473..516)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      697..754
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(778..795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(949..1537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1029..1048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1720..1814)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1835..2205,1..289))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
       61 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      121 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      181 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      241 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
      301 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      361 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
      421 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
      481 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc aggagggagg
      541 cgtgtcgttt tagtgaggct tacgtctggg attacgtcta cttacgtctg ggattacgtc
      601 tacttacgtc tgggattacg tctactatat aaggttttgc tattcattga aagcagtagt
      661 gactgatttg tatataatgt actcgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc
      721 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1681 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1741 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1801 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1861 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1921 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1981 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     2041 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     2101 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2161 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgct
//
