LOCUS       sequence-386879-        2205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: MinSyn_302.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYC0CM0519
FEATURES             Location/Qualifiers
     source          1..2205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     179..198
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     426..444
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(448..491)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      672..729
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(753..770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(924..1512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1004..1023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1695..1789)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1810..2205,1..264))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
       61 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      121 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      181 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      241 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
      301 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
      361 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      421 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      481 aggatgattt ctggaattcg gtctcaggag ggaggcgtgt cgttttagtg aggctgacgt
      541 aagggatgac gtaaacttac gtctgggatt acgtctactt acgtctggga ttacgtctac
      601 tatataaggt tttgctattc attgaaagca gtagtgactg atttgtatat aatgtactcg
      661 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      721 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      901 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1681 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1741 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1801 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1861 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1921 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1981 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     2041 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     2101 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     2161 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtg
//
