LOCUS       sequence-386880-        2205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: MinSyn_303.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYC0CM0520
FEATURES             Location/Qualifiers
     source          1..2205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     557..575
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(579..622)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      803..860
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(884..901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1055..1643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1135..1154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1826..1920)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1941..2205,1..395))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
       61 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      121 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      181 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      241 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      301 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      361 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      421 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      481 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
      541 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      601 agctttcgct aaggatgatt tctggaattc ggtctcagga gggaggcgtg tcgttttagt
      661 gaggcttacg tctgggatta cgtctactga cgtaagggat gacgtaaact tacgtctggg
      721 attacgtcta ctatataagg ttttgctatt cattgaaagc agtagtgact gatttgtata
      781 taatgtactc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
      841 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
      901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     1081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1801 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga
     1861 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     1921 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     1981 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     2041 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     2101 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     2161 gctgagacga aaaacatatt ctcaataaac cctttaggga aatag
//
