LOCUS       sequence-386882-        3165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: AtTGA1_CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0350
FEATURES             Location/Qualifiers
     source          1..3165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(89..748)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     663..682
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     910..928
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(932..975)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2116..2173
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2197..2214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2368..2956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2448..2467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(3139..3165,1..68))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
       61 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
      121 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
      181 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
      241 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
      301 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
      361 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
      421 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
      481 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
      541 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
      601 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
      661 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
      721 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
      781 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
      841 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta
      901 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
      961 gctaaggatg atttctggaa ttcggtctca aagcctacgt tggttcacga tgtcgagttg
     1021 cccaacttga gctcaaggct ctaagccgtt gaaaatactc accaagagct aataatcctc
     1081 gagccgcttg tcgcgttgtc aatatccgat acatttgttg caatgtttca tgtctcaagt
     1141 gatcagcctg atttacgaaa ctgaccaaag cttctaatct atccatagca gaattcacct
     1201 gaggaatgta acttccttca ccgagttgtc ccgctgcaac gcagtccgca agggtgtgtt
     1261 gcagcttctc cataccttga gtcaacgcgt cttctgcttg ctgacacgat tgttttagat
     1321 tgcatacatc tagaagttgt tgatccgtca agacatcaaa atgtggcaaa agaaccttga
     1381 gaagatcgga gggtcgaaat ccgccaatcc ataagaagaa tcgttctgct gaagttctcc
     1441 acatccctga catgacgaag aagacatcgg ctttggcagc agacgatttc atccggaaaa
     1501 gctcaaagta atgtttcatg gcgttttcga ctagcgaacg aagctcgata tcgttaatgt
     1561 gtccgtgtaa aactgttctt agttcacata tctgtctgtt ctgttcttca acccaatgtc
     1621 catattccat ttcaaatgca gcaatccctg gattcatggt ttccgaaaaa ccgagagaat
     1681 tagtatctat tccgtttcct acatagaatc cctgttgtct agcacgatcg agttcttgct
     1741 ctaattgaat tagcttcaac ctgcttgttt ccagttgctg aacataagcc ttcttgcgca
     1801 agcgactttt cctagcagcc tcgcggtttt gagcaagccg tctttgtatc ttatcgggat
     1861 gtctagacgt tgaagcttct tgatcgaaca tgtgaggagt tcctgctgtt ccatgggaag
     1921 tatcctctga cacgttgttg tctagtttct gattattcgg tattattatg tggtttggtg
     1981 tgttcatagt cccattgcta atattgcttt tgaaactctc cccccacata ccgaattgat
     2041 ggacaggttc gtatatacca actcttctcg gtggcacaaa atgtgtcgat gtcgaattca
     2101 tttgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     2161 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     2221 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2281 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2341 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     2401 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2461 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2521 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2581 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2641 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2701 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2761 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2821 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2881 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2941 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3001 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3061 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3121 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcg
//
