LOCUS       sequence-386883-        6292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Arabidopsis NLP7 CDS and c-terminal HiBiT 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYCeGM0010
FEATURES             Location/Qualifiers
     source          1..6292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3355..3454)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3566..3585)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3644..3663)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(4237..4770)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4959..4964,4965..4981,4982..4987))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      5644..6232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6133..6152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagcagg cttcatgtgc gagcccgatg ataattccgc tagaaacggc gtcactactc
      481 aaccttcgag gtcaagggag cttctaatgg atgttgacga cttagatctt gacggttcat
      541 ggccactaga tcaaatccct tacttatcct catcgaatcg catgatttct ccgatttttg
      601 tctcctcttc ctctgagcag ccttgctcgc ctctctgggc tttctccgac ggtggaggaa
      661 atggttttca ccacgcaacc tccggtggcg atgatgagaa gatcagctct gtctccggtg
      721 ttccttcttt ccgtctcgcc gagtatcctc tcttcctccc ttactcttct ccatcagcag
      781 ctgagaacac aacagagaag cataacagtt tccagtttcc gtctccattg atgagcctag
      841 tcccaccaga gaacacagac aactactgtg tgatcaaaga gaggatgact caggcgcttc
      901 gatacttcaa agaatcaacc gaacaacacg ttttggctca ggtctgggct cctgtgagaa
      961 agaatggtcg tgatttgctg acgactttgg gtcaaccttt tgttcttaat cctaatggta
     1021 atgggcttaa tcaatacagg atgatctctc tcacatatat gttttctgtg gatagtgaaa
     1081 gtgacgtaga gctcggactc ccgggtcgag ttttccgtca gaaattgcct gaatggactc
     1141 caaatgttca gtactattcc agcaaagaat tctcgcggct tgatcacgcc ttgcactaca
     1201 acgtgcgtgg tacactggcc ttgcctgtct ttaatccctc tggtcagtcc tgcataggtg
     1261 ttgtggaact tataatgacc tcagagaaga ttcactatgc acccgaagtg gacaaagttt
     1321 gcaaagccct tgaggcggta aatctgaaaa gctcggaaat acttgatcac caaacaacac
     1381 agatatgcaa tgagagtcgc caaaacgcgc ttgctgagat tctcgaagtg ctgacagttg
     1441 tatgtgaaac ccataacttg cctctcgctc agacttgggt tccatgtcag catgggagcg
     1501 ttcttgccaa tggtggcggt ctaaagaaaa actgcaccag ctttgacggt agctgcatgg
     1561 gtcaaatctg catgtctaca accgacatgg cctgctatgt cgtggatgct catgtctggg
     1621 gctttagaga tgcctgtctt gaacaccatc tccagaaagg ccagggagtc gctggacgag
     1681 cttttctcaa tggtggctca tgtttctgca gagacatcac caagttctgc aaaacgcagt
     1741 acccactagt ccattatgcg ctcatgttca agttgaccac ttgttttgca atatctctcc
     1801 agagctctta cacgggcgac gacagttaca ttcttgaatt ttttcttcct tcgagtataa
     1861 cagacgacca agagcaagat ttgctgttgg gttctatttt ggtgacaatg aaagaacatt
     1921 ttcagagtct gagggttgca tctggggttg actttggtga agatgacgac aaattgtctt
     1981 tcgagatcat ccaagcatta ccggacaaga aggttcattc aaaaatagaa tccattcgag
     2041 ttcccttttc tggttttaag tcaaatgcaa cagagacgat gttgattcct cagcctgtgg
     2101 ttcagtcttc tgatccagta aatgagaaaa tcaacgtggc cactgttaac ggtgtggtta
     2161 aggagaagaa gaaaacagag aaaaagcgtg ggaagactga gaaaacaatc agtctagatg
     2221 tacttcagca gtatttcact ggaagtctca aagacgctgc aaagagccta ggagtttgcc
     2281 cgacgacaat gaagcgaatt tgcaggcaac acggaatctc gcggtggcca tcgaggaaga
     2341 tcaagaaagt gaatcgttca atcacaaagc tgaaacgagt catcgaatct gttcaaggta
     2401 ctgatggagg cctcgacctg acttccatgg ccgttagttc catcccttgg acacacggtc
     2461 aaacatcagc acagccacta aactcaccca atggttccaa accacctgag ctaccaaaca
     2521 ccaataattc acctaaccat tggtcaagtg atcacagtcc gaacgagcca aatggttcgc
     2581 ctgagttacc accaagcaat ggtcacaagc gatcacgaac ggtggatgag agcgctggga
     2641 ctccaacctc tcatggctca tgtgacggta accaattaga tgaaccgaaa gtcccaaatc
     2701 aagatccgct tttcacggtt ggtggatcac ccgggctcct ttttccacct tattctagag
     2761 atcatgatgt atctgcagct tccttcgcaa tgccgaacag gcttcttggt tctatagacc
     2821 atttccgagg aatgctcatt gaagacgctg gaagttcaaa agatctgaga aatctctgcc
     2881 ccactgcagc atttgacgat aagtttcaag acacaaactg gatgaacaat gataataata
     2941 gcaacaacaa cttatacgct cccccaaagg aagaggccat tgcaaatgtt gcatgcgaac
     3001 catcaggctc agaaatgaga acggtaacaa tcaaagcaag ttacaaagac gacataatac
     3061 ggttcagaat atcctcgggt tcaggtataa tggaattgaa ggatgaagtg gctaagaggc
     3121 tgaaagttga tgcaggaacg ttcgatatca agtatcttga cgatgataac gaatgggttt
     3181 taatagcttg tgatgctgat cttcaagaat gtctcgagat ccctagatcc tcccgcacga
     3241 aaatcgtaag gctcttagtt catgatgtaa cgacaaatct agggagctcc tgcgagagca
     3301 ctggagaatt gggttcggtg agcggctggc ggctgttcaa gaagattagc tgaaacccag
     3361 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     3421 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg
     3481 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     3541 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     3601 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     3661 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     3721 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3781 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     3841 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     3901 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     3961 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     4021 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     4081 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     4141 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     4201 ttaggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     4261 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     4321 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     4381 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     4441 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     4501 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     4561 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     4621 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     4681 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     4741 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     4801 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     4861 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     4921 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     4981 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     5041 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     5101 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     5161 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     5221 tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg
     5281 cccaggactc tagctatagt tctagtggtt ggctactaat aggttgtatt gatgttggac
     5341 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     5401 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     5461 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     5521 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     5581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5761 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tg
//
