LOCUS       sequence-386884-        5938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Arabidopsis NLP6 CDS and c-terminal HiBiT 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYCeGM0009
FEATURES             Location/Qualifiers
     source          1..5938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3001..3100)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3212..3231)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3290..3309)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3883..4416)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4605..4610,4611..4627,4628..4633))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      5290..5878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5779..5798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagcagg cttcatggaa cttgacgact tggatctcag cggttcttgg ccgttagatc
      481 agatcacttt tgcttcaaat ttcaagtctc ctgtcatttt ctcttcctcc gaacaacctt
      541 tctctcctct ctggtccttc tccgaaactt ctggagatgt cggcggcgag ctttactccg
      601 ccgcagtagc tcccactcgc ttcaccgatt attccgtctt actcgctagt tctgaatctg
      661 aaaccacgac caaggagaat aatcaggttc cttctccgtc atggggtata atgcctttgg
      721 agaatccaga tagttactgt gccatcaaag ccaagatgac tcaagcgctt agatacttca
      781 aggaatctac tggacaacaa catgttcttg ctcaggtttg ggcgcctgtc aaaaaccgtg
      841 gtcgatatgt tcttacaact tcaggacagc catttgttct tggtcccaat agtaatggcc
      901 ttaatcaata caggatggtt tcattgacgt atatgttttc ccttgatggt gaacgggacg
      961 gagagctcgg gcttcctggt cgcgttttca ggaaaaagtt acctgagtgg actcctaatg
     1021 tgcagtatta ctccagcaaa gagttttcaa ggcttggtca cgccttgcac tataatgttc
     1081 agggaacttt ggctttgcct gtttttgaac catccaggca actctgtgta ggagttgtcg
     1141 agcttattat gacttctccg aagattaact atgctcctga ggttgaaaaa gtttgtaaag
     1201 cgcttgaggc cgtgaatctg aaaacttcgg aaatattgaa ccacgagact actcagatct
     1261 gcaacgaggg tcgccaaaat gcgttagctg agattttgga gatactgaca gtggtgtgcg
     1321 aaacctacaa actgccttta gcccagacat gggtcccttg caggcatcgc agtgttctag
     1381 cctttggagg tggttttaag aaaagctgtt cgagcttcga tggaagctgt atggggaaag
     1441 tctgtatgtc tactagtgat ttagcggttt atgttgttga tgctcatgtt tggggtttcc
     1501 gagacgcctg tgctgaacac catctgcaga agggacaagg tgttgctggg agggcgtttc
     1561 agtctggaaa tctatgcttc tgtagagatg ttactcggtt ctgcaaaacc gactacccat
     1621 tagtacacta tgcacggatg ttcaagttga cgagctgttt cgcggtctgc ttaaagagca
     1681 catatacagg ggatgatgag tacgttcttg aattctttct tcctccagcc atcacagaca
     1741 agagtgagca agattgtcta ttgggttctc tgcttcagac aatgaagcag cactattcga
     1801 gtctcaaggt tgtctctgaa actgaactct gtgaaaacaa catgtctctt gaagtcgtgg
     1861 aagcttcgga ggacggaatg gtttactcga aacttgaacc aatcaggata catcatccag
     1921 cacaaatctc taaggattac cttgagctca acgcacccga acagaaggtg agcttaaact
     1981 ctgactttat ggagaataat gaagttgatg atggggttga gaggttccaa acgcttgatc
     2041 ctataccaga ggccaaaaca gtgaaaaaat cagaaaggaa gcgtgggaaa acagagaaaa
     2101 cgatcagtct agaagtgctt caacaatatt ttgctggtag tctaaaagat gcagcaaaga
     2161 gcctcggagt ttgcccaaca acaatgaaac gcatttgccg acaacacggg atctcacggt
     2221 ggccatctcg aaaaatcaac aaggtgaacc gatctctcac acgacttaaa catgttatag
     2281 actcggttca aggtgctgat ggatcactca acttgacgtc cctctctcct cgcccttggc
     2341 ctcaccaaat ccctccaatc gatatccaat tagcaaagaa ctgccctcca acttctacat
     2401 ctcccctaag taatctccaa gatgtcaaaa tcgaaaacag agatgcagaa gacagtgcag
     2461 gttcatcaac atcgcgtgct tcttgcaaag tgaatcctat ttgcgagacg cgttttcggc
     2521 ttcctacaca caaccaagaa ccatccagac aagtggcctt ggacgattcg gatagtagct
     2581 ctaaaaacat gacaaatttt tgggcacact taacctgcca agatacagct tctccaacaa
     2641 ttctccaaca taagctcgtg agtataaagg caacgtacag agaagacatc atcaggttca
     2701 agatctcacc agagtcggta agcattacgg aacttaagca gcaagtggcc aaaaggctga
     2761 agctcgaaac ggcagccttt gagctcaagt atctagacga cgatagagaa tgggtgtccg
     2821 tctcatgcga tgctgacctc agcgaatgcc tcgacacgtc tgcagcaaaa gcgaacacac
     2881 tgaggctctc agtacatgac gtgactttta actttgggag ctcatgcgaa agctcagagg
     2941 aaactatgat gtgcttgggt tcggtgagcg gctggcggct gttcaagaag attagctgaa
     3001 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     3061 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg
     3121 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     3181 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     3241 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     3301 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     3361 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     3421 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     3481 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     3541 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     3601 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     3661 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     3721 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     3781 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     3841 gggaaattag gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     3901 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     3961 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     4021 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     4081 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     4141 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     4201 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     4261 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     4321 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     4381 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     4441 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     4501 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     4561 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     4621 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     4681 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
     4741 cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
     4801 gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg
     4861 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
     4921 tgttcgccca ggactctagc tatagttcta gtggttggct actaataggt tgtattgatg
     4981 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     5041 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     5101 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     5161 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     5221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatg
//
