LOCUS       sequence-386885-        4405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Arabidopsis TGA2 CDS and c-terminal HiBiT 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYCeGM0002
FEATURES             Location/Qualifiers
     source          1..4405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1468..1567)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1679..1698)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1757..1776)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2350..2883)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3072..3077,3078..3094,3095..3100))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3757..4345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4246..4265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagcagg cttcatggct gataccagtc cgagaactga tgtctcaaca gatgacgaca
      481 cagatcatcc tgatcttggg tcggagggag cactagtgaa tactgctgct tctgattcga
      541 gtgaccgatc gaagggaaag atggatcaaa agactcttcg taggcttgct caaaaccgtg
      601 aggcagcaag gaaaagcaga ttgaggaaga aggcttatgt tcagcagcta gagaacagcc
      661 gcttgaaact aacccagctt gagcaggagc tgcaaagagc aagacagcag ggcgtattca
      721 tttcaggcac aggtgaccag gcccattcta ctggtggaaa tggtgctttg gcgtttgatg
      781 ctgaacattc acggtggttg gaagaaaaga acaagcaaat gaacgagctg aggtctgctc
      841 tgaatgcgca tgcaggtgat tctgagcttc gaataatagt cgatggtgtg atggctcact
      901 atgaggagct tttcaggata aagagcaatg cagctaagaa tgatgtcttt cacttgctat
      961 ctggcatgtg gaaaacacca gctgagagat gtttcttgtg gctcggtgga tttcgttcat
     1021 ccgaacttct aaagcttctg gcgaatcagt tggagccaat gacagagaga cagttgatgg
     1081 gcataaataa cctgcaacag acatcgcagc aggctgaaga tgctttgtct caagggatgg
     1141 agagcttaca acagtcacta gctgatactt tatcgagcgg gactcttggt tcaagttcat
     1201 cagggaatgt cgcaagctac atgggtcaga tggccatggc aatgggaaag ttaggtacac
     1261 tcgaaggatt tatccgccag gctgataatt tgagactaca aacattgcaa cagatgataa
     1321 gagtattaac aacgagacag tcagcacgtg ctctacttgc aatacacgat tacttctcac
     1381 ggctacgagc tctaagctcc ttatggcttg ctcgacccag agagggttcg gtgagcggct
     1441 ggcggctgtt caagaagatt agctgaaacc cagctttctt gtacaaagtt ggcattataa
     1501 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1561 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1621 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1681 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1741 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1801 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1861 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1921 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1981 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2041 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2101 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2161 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2221 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2281 tcacttgata accttatttt tgacgagggg aaattaggcc gggaagccga tctcggcttg
     2341 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     2401 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     2461 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     2521 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
     2581 ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     2641 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     2701 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
     2761 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     2821 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     2881 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     2941 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     3001 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     3061 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     3121 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt
     3181 cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct
     3241 tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa
     3301 gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc
     3361 tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg
     3421 gttggctact aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     3481 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     3541 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3601 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3661 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3901 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4381 cttttgctgg ccttttgctc acatg
//
