LOCUS       sequence-386887-        4105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Arabidopsis DREB26 CDS and c-terminal 
            HiBiT tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYCeGM0011
FEATURES             Location/Qualifiers
     source          1..4105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1168..1267)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1379..1398)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1457..1476)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2050..2583)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2772..2777,2778..2794,2795..2800))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3457..4045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3946..3965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagcagg cttcatggtg aaacaagaac gcaagatcca aaccagcagc acaaaaaagg
      481 aaatgccttt gtcatcatca ccatcttctt cttcttcttc atcttcttcc tcgtcttcgt
      541 cttcgtgtaa gaacaagaac aagaagagta agattaagaa gtacaaagga gtgaggatga
      601 gaagttgggg atcatgggtt tctgagatta gggcaccaaa tcaaaagaca aggatttggt
      661 taggttctta ctcaacagct gaagcagctg ctagagctta cgatgttgca ctcttatgtc
      721 tcaaaggccc tcaagccaat ctcaacttcc ctacttcttc ttcttctcat catcttcttg
      781 ataatctctt agatgaaaat acccttttgt cccccaaatc catccaaaga gtagctgctc
      841 aagctgccaa ctcatttaac cattttgccc ctacttcatc agccgtctcg tcaccgtccg
      901 atcatgatca tcaccatgat gatgggatgc aatctttgat gggatctttt gtggacaatc
      961 atgtgtcttt gatggattca acatcttcat ggtatgatga tcataatggg atgttcttgt
     1021 ttgataatgg agctccattc aattactctc ctcaactaaa ctcgacgacg atgctcgatg
     1081 aatacttcta cgaagatgct gacattccgc tttggagttt caatggttcg gtgagcggct
     1141 ggcggctgtt caagaagatt agctgaaacc cagctttctt gtacaaagtt ggcattataa
     1201 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1261 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1321 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1381 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1441 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1501 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1561 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1621 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1681 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1741 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1801 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1861 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1921 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1981 tcacttgata accttatttt tgacgagggg aaattaggcc gggaagccga tctcggcttg
     2041 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     2101 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     2161 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     2221 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
     2281 ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     2341 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     2401 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
     2461 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     2521 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     2581 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     2641 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     2701 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     2761 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     2821 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt
     2881 cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct
     2941 tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa
     3001 gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc
     3061 tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg
     3121 gttggctact aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     3181 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     3241 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3301 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3361 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4081 cttttgctgg ccttttgctc acatg
//
