LOCUS       sequence-386889-        4519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYCeGM0001
FEATURES             Location/Qualifiers
     source          1..4519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1582..1681)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1793..1812)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1871..1890)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2464..2997)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3186..3191,3192..3208,3209..3214))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3871..4459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4360..4379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagcagg cttcatgaat tcgacatcga cacattttgt gccaccgaga agagttggta
      481 tatacgaacc tgtccatcaa ttcggtatgt ggggggagag tttcaaaagc aatattagca
      541 atgggactat gaacacacca aaccacataa taataccgaa taatcagaaa ctagacaaca
      601 acgtgtcaga ggatacttcc catggaacag caggaactcc tcacatgttc gatcaagaag
      661 cttcaacgtc tagacatccc gataagatac aaagacggct tgctcaaaac cgcgaggctg
      721 ctaggaaaag tcgcttgcgc aagaaggctt atgttcagca actggaaaca agcaggttga
      781 agctaattca attagagcaa gaactcgatc gtgctagaca acagggattc tatgtaggaa
      841 acggaataga tactaattct ctcggttttt cggaaaccat gaatccaggg attgctgcat
      901 ttgaaatgga atatggacat tgggttgaag aacagaacag acagatatgt gaactaagaa
      961 cagttttaca cggacacatt aacgatatcg agcttcgttc gctagtcgaa aacgccatga
     1021 aacattactt tgagcttttc cggatgaaat cgtctgctgc caaagccgat gtcttcttcg
     1081 tcatgtcagg gatgtggaga acttcagcag aacgattctt cttatggatt ggcggatttc
     1141 gaccctccga tcttctcaag gttcttttgc cacattttga tgtcttgacg gatcaacaac
     1201 ttctagatgt atgcaatcta aaacaatcgt gtcagcaagc agaagacgcg ttgactcaag
     1261 gtatggagaa gctgcaacac acccttgcgg actgcgttgc agcgggacaa ctcggtgaag
     1321 gaagttacat tcctcaggtg aattctgcta tggatagatt agaagctttg gtcagtttcg
     1381 taaatcaggc tgatcacttg agacatgaaa cattgcaaca aatgtatcgg atattgacaa
     1441 cgcgacaagc ggctcgagga ttattagctc ttggtgagta ttttcaacgg cttagagcct
     1501 tgagctcaag ttgggcaact cgacatcgtg aaccaacggg ttcggtgagc ggctggcggc
     1561 tgttcaagaa gattagctga aacccagctt tcttgtacaa agttggcatt ataagaaagc
     1621 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1681 gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1741 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1801 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1861 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1921 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1981 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2041 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2101 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2161 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2221 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2281 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2341 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2401 gataacctta tttttgacga ggggaaatta ggccgggaag ccgatctcgg cttgaacgaa
     2461 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
     2521 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
     2581 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
     2641 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
     2701 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
     2761 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     2821 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     2881 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     2941 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     3001 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     3061 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     3121 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     3181 attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc
     3241 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc
     3301 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt
     3361 tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc
     3421 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt
     3481 ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc
     3541 tactaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     3601 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     3661 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     3721 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     3781 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3841 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3901 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3961 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4021 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4081 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4141 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4201 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4261 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4321 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4381 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4441 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4501 ctggcctttt gctcacatg
//
