LOCUS       sequence-386890-        5221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Arabidopsis ARF18 CDS and c-terminal HiBiT
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYCeGM0022
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    333..432
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2284..2383)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2495..2514)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2573..2592)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3166..3699)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3888..3893,3894..3910,3911..3916))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4573..5161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5062..5081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      421 aaaaagcagg cttcatggcg agtgttgaag gtgatgatga tttcggaagt tcttcgtcaa
      481 ggtcttatca agatcaacta tacacagagc tatggaaagt ttgtgcaggt ccattagtgg
      541 aagttcctcg tgctcaagag agagttttct acttccctca gggtcacatg gaacaacttg
      601 tggcgtcaac taatcaagga atcaattcag aagaaatacc tgtttttgat cttcctccaa
      661 agatactttg tcgagttctt gatgtcactt taaaggcgga gcatgaaaca gatgaggttt
      721 acgctcagat cacattacaa ccagaggaag atcaaagtga accaacaagt cttgatccac
      781 ctattgttgg accaactaag caagagtttc attcgtttgt taagatttta acggcttcag
      841 atacaagcac tcatggtgga ttctctgttc ttcgtaaaca cgccactgaa tgcttgcctt
      901 ctttggatat gacacaagct actcctactc aagaacttgt gactagagat cttcatggct
      961 ttgaatggag gtttaagcat atattcagag gacaaccacg gaggcatttg cttactacgg
     1021 gttggagtac atttgtatcc tcgaaaagac ttgtagctgg agatgctttt gtgttcttga
     1081 ggggtgagaa tggggattta cgggttggag tgagacgatt agctcggcat caaagcacaa
     1141 tgcctacttc ggttatttca agtcagagca tgcatttggg agttcttgct acagcttctc
     1201 atgctgtgcg tacaacaaca atctttgttg tcttttacaa gcctaggata agccaattca
     1261 tagttggggt gaacaagtat atggaagcta taaagcatgg attttctctc ggtacccgat
     1321 tcagaatgag gtttgaagga gaagagtctc ctgagagaat atttactggt acgattgtgg
     1381 gaagtggaga tctatcttca caatggccag cttctaaatg gaggtcattg caggtacaat
     1441 gggatgagcc aacaacagtt caaagaccag ataaagtctc accatgggag atagagcctt
     1501 tcttggcaac ttccccaatt tcaactcctg ctcaacaacc acaatcgaaa tgcaagcggt
     1561 caagacccat cgagccatca gttaaaacac cagccccacc tagtttcttg tacagcctcc
     1621 ctcagagcca agattccatt aatgcatccc ttaaactgtt tcaagatcca tcacttgaga
     1681 gaatttcagg tggatactcc tcaaacaaca gcttcaaacc cgagactcct cctcctccaa
     1741 cgaattgtag ctataggttg tttggatttg atctcacaag caattctcct gctccaatcc
     1801 ctcaagacaa gcaaccgatg gatacttgtg gagctgccaa gtgtcaagaa cccatcactc
     1861 caacctcaat gagtgagcag aagaagcaac aaacatcaag aagtcgaact aaagtgcaaa
     1921 tgcaaggcat tgcggttggt cgtgcggttg atttaacact gttgaaatct tacgatgaac
     1981 tgattgatga gcttgaggag atgtttgaga ttcaaggaca gcttcttgcc cgagacaaat
     2041 ggatcgttgt cttcactgat gatgaaggag atatgatgct tgctggtgat gatccgtgga
     2101 atgagttttg caagatggca aagaagatat ttatatattc gagcgatgag gttaagaaaa
     2161 tgacaacgaa actgaagatt tcttcgtcgt tagagaatga ggaatatggt aatgaatcat
     2221 tcgaaaatcg tagtaggggg ggttcggtga gcggctggcg gctgttcaag aagattagct
     2281 gaaacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2341 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc
     2401 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2461 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2521 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2581 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2641 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2701 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2761 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2821 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2881 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2941 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     3001 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     3061 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     3121 gaggggaaat taggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     3181 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     3241 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     3301 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     3361 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     3421 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     3481 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     3541 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     3601 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     3661 actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca
     3721 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
     3781 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     3841 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
     3901 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     3961 caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg
     4021 tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc
     4081 tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc
     4141 cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc
     4201 gtttgttcgc ccaggactct agctatagtt ctagtggttg gctactaata ggttgtattg
     4261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     4321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     4381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     4441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     4501 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4681 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5221 g
//
