LOCUS       sequence-38691-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane localized EGFP under the Dusp6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Wang G, Cadwallader AB, Jang DS, Tsang M, Yost HJ, Amack JD
  TITLE     The Rho kinase Rock2b establishes anteroposterior asymmetry of the 
            ciliated Kupffer's vesicle in zebrafish.
  JOURNAL   Development. 2011 Jan;138(1):45-54. Epub 2010 Nov 23.
  PUBMED    21098560
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-01"; volume: "138(1)"; pages: "45-54. 
            Epub 2010 Nov 23"
COMMENT     Sequence Label: pISceI Dusp6:memGFP
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     44..60
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     45..61
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
       61 gataagcttg atatcgaatt catcatacga attagccact aaatgcaaaa gttacgaatt
      121 gctgtgagat tgtgttgcac atttgcatgc aaaaacagta caacgtaaaa agtgtgtgct
      181 gtgtgtgggt ctgtccactg tgtgtgtctc tgtgaacttt gtaatgacat tatgtgtgac
      241 tcatcaaaag gcttacatta accccacaac aaatacatca aataatcatt ggaaaagttc
      301 ttactgtagt atttctcaca aatattacgt gagatctgct tccttcatgt ctgtcactgt
      361 gctgtttatc ttacacagct gaggcggaga ttgaggcaca ctatgactgg cacatgggaa
      421 cggtgggtgg ggaaaaccag cattaaaggc acagaccaca aaaacagcta caagatgctc
      481 agagctgatg ggtgttttga gctgaaactt cacagacaca ttctcgagaa aaaaaatctt
      541 aaatcttgaa aaaggggtaa attaggtgcc ctttaagcta aatatttttc ctatatatat
      601 atatatatat atatatatat atatatatat atatatatat atatatatat ata
//
