LOCUS       sequence-386935-       11402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse SAFB lacking the rrm domain in mammalian cells. 
            Contains a c-terminal 3xFlag tag and a c-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: CAGGS-safb∆rrm-NLS-3XFLAG-V5
FEATURES             Location/Qualifiers
     source          1..11402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1444..1747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1749..2026
                     /label=chicken beta-actin promoter
     intron          2027..3043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2967..2989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3051..3070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3116..3125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5717..5737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5744..5809
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5828..5869
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    5899..6123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6251..6341
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        6389..6600
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6613..6881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6918..7943
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7972..8093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8009..8028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8063..8082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8856..8890
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9381..9397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9381..9397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9394..9416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9405..9421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9429..9435,9436..9453,9454..9459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9474..9495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9612..9629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9783..10371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9863..9882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10542..11333,11334..11402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11165..11184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagtggatcc attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1801 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgrkgg gggsgggggg
     1861 gggggggggg cgsgcgcsgg gsgggggggg gcggggcgag gggcggggcg gggcgaggcg
     1921 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1981 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2041 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2101 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2161 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2221 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2281 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2341 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2401 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2461 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2521 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2581 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     2641 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2701 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2761 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2821 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2881 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2941 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3001 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3061 ggttattgtg ctgtctcatc attttggcaa acctaggatc ggtactgttg gtaaagccac
     3121 catggcggag actctgtcgg gtttaggaga cgcgagtgcg gcgggcgcgg cggccgtgag
     3181 ctccgcggcg tcagagaccg ggacgcggcg gctcagcgac ctgcgggtga tcgacctgcg
     3241 ggcggagctg aagaagcgga acctcgactc gagcggcaac aagagcgtgc tgatggagcg
     3301 gctgaagaag gccattgaag atgaaggtgg gaatcctgat gaaattgaag tcacctcaga
     3361 atgcaacaag aagatgccta agaggcccag caaagggagg aaaccagaag acgagggtgt
     3421 ggaggacaac gggctcgagg agaattctgg ggatgggcag gaggatgtgg agaccagcct
     3481 ggagaacctg caagacatgg atatgatgga catcagtgtg ctggatgagg ctgacattga
     3541 caatggcagc gtggcagact gtgtggagga agaggaggag gccacactcc ctgagggcct
     3601 agcagacagc actgaactgg tagaaggaga tttgaaaggg ctcccagaac agctgcagga
     3661 gcatgctata gatgacaagg acaccgttaa caatgtagat acgtcatcat ctgacttcac
     3721 catgctgcag gagatggaag aggcatcttt ggagccagaa aatgagaaaa tactcgacat
     3781 tttgggggaa acttgtaaat ctgagccagt aaaagaagaa ggttcggagc tggagcagcc
     3841 ctttgcccag gccacgagta gcgtggggcc agacaggaag ctggcggagg aagaggacct
     3901 atttgagagc tgcggccacc cggaagagga agaggaagag gaggaagaag atcaggaaga
     3961 ggagcaggag gaggagggag atttagcttt ggccagcagc agcaagtctg agtctccaag
     4021 cactcggtgt cagtggagcg aggcagatgc cccgttagca gtagtgaaaa gggagccggc
     4081 agatgcgcct ggcggaggca ctgggatgga ccgcgagcct gtagggctag aggagccagt
     4141 tgaacagagt agcacggctg cccagctccc ggaggccacc agccaggagc tggtgcgagc
     4201 gcccacggca gccctgagcc ctgagcccca agatagcaaa gaagacgtga agaagtttgc
     4261 ttttgacgct tgtaatgacg tccctgcgcc tcctaaagag tcctcagcca gtgagggcgc
     4321 tgatcagaaa atgagttctg tggaggagga ctcggataca aaaaggcttt cccgagaaga
     4381 gaagggtcgc agcagctgtg gtgagcccac tggcaaaaga gtgcctgaca ggcgagatgg
     4441 ggacagcaag aaggagaagg ccagcaccag tgacagatct gcaaacctta agagggagga
     4501 gaaaggtgag agaaaagacg atgccaagaa gacagatgat gggagcacag agaagagcaa
     4561 agacgcagat gaccagaaac ctgggccttc agagcgctcg cgaacgacca agtcaggaag
     4621 ccgtggcact gagcgcactg tggtgatgga caagtccaaa ggtgtgcctg tcatcagtgt
     4681 gaaaacatct gggtctaaag agagagcctc caaaagtcag gaccggaagt cagccagccg
     4741 ggagaagcgg tctgttgtgt cctttgacaa agttaaagag tctcgaaagt ccagagactc
     4801 agagtctcgg agggaacggg agcgcagtga gcgggagcag aggctgcagg cccagtggga
     4861 gcgggaggag cgagagcggc tggagattgc tcgtgagcgc ttggcattcc accgccatcg
     4921 gctagagcgt gaacgcatgg agcgggagcg gctggagcgg gagcggatgc atgtagaaca
     4981 ggagcggagg cgtgagcaag agcgcattca ccgagagcga gaggagcttc ggcgtcagca
     5041 ggagctgcgc tatgaacagg agcgccggcc tgctgtgcgc aggccctatg aagtggatgg
     5101 ccggcgagat gatgcctatt ggccagaagc taaacgggct gctctggatg accgttacca
     5161 ctcagacttc agccgccagg accgctttca cgacttcgac cacagagaca gaggccgtta
     5221 tcccaaccat tctgtggata ggagagaagg ttcacggtcg atgatgggag acagagaagg
     5281 acagcattac cctgaacgcc atggaggacc tgaacgccat ggccgggact ctcgagatgg
     5341 ctggggctat ggttcgaaca agagattgag tgaaggccgg gggctgcccc ctcctcccag
     5401 gcgtgactgg ggggagcatg gccggcgact tgaagatgac cgggcatggc agggcacggc
     5461 tgatggaggc atgatggagc gtgatcataa gaggtggcaa ggtggtgaga ggagcatgtc
     5521 tggccactcg gggcctggtc acatgatgaa ccgtggtggc atgtcagggc gaggcagttt
     5581 tgccccagga ggagcttctc gtggccatgt gatcccacgt ggagggatgc aggcaggctt
     5641 tggaggacag agccggggaa gcagacccag tgatgcccgc ttcacccgcc gctatggccc
     5701 tggtaccgcg gccgctccca agaagaagag gaaagtctcg agcgattaca aagaccatga
     5761 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagg gctcgggctc
     5821 gacctcgggt aagcctatcc ctaaccctct cctcggtctc gattctacat gaaatctaga
     5881 gtcgacaatg cgtttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5941 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6001 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6061 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6121 tggaaacacg cggcgacgcg ttcgaccgaa taaaacctgt gacggaagat cacttcgcag
     6181 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
     6241 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     6301 taccgggcgt attttttgag ttgtcgagat tttcaggagc taaagatctg gaagctaagg
     6361 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6421 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     6481 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6541 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6601 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6661 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6721 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6781 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6841 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6901 acaagctgct agccaccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     6961 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     7021 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     7081 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     7141 cggaagtgct tgacattggg gagttcagcg agagcctgac ctattgcatc tcccgccgtg
     7201 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     7261 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     7321 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     7381 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     7441 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     7501 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     7561 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     7621 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     7681 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     7741 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     7801 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     7861 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     7921 ctcgtccgag ggcaaaggaa tagttttacg tattgagtat ttaactaact aaacttgttt
     7981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8101 tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc tcccttccgg
     8161 gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg gttcttatat
     8221 cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg aagtccctaa
     8281 agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt tagggcccat
     8341 tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     8401 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     8461 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     8521 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     8581 tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
     8641 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
     8701 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     8761 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     8821 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     8881 ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag
     8941 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     9001 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     9061 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     9121 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     9181 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     9241 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     9301 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     9361 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11401 at
//
