LOCUS       sequence-38694-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane localized EGFP under the Dusp6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Wang G, Cadwallader AB, Jang DS, Tsang M, Yost HJ, Amack JD
  TITLE     The Rho kinase Rock2b establishes anteroposterior asymmetry of the 
            ciliated Kupffer's vesicle in zebrafish.
  JOURNAL   Development. 2011 Jan;138(1):45-54. Epub 2010 Nov 23.
  PUBMED    21098560
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-01"; volume: "138(1)"; pages: "45-54. 
            Epub 2010 Nov 23"
COMMENT     Sequence Label: pISceI Dusp6:memGFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    176..310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(213..232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     267..286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(460..481)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     809..828
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtaacggc cgccagtgtg ctggaattcg cccttaaaac tagtgccgcg aattaaaaaa
      121 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      181 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      241 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tattttatca
      301 tgtctggatc tacgtaatac gactcactat agttctagat caggagagca cacacttgca
      361 gctcatgcag ccggggccac tctcatcagg agggttcagc ttagatcttc ctcctccctt
      421 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      481 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
      541 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      601 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
      661 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
      721 caggatgttg ccgtcctcct tgaagttgat gcccttcagc tcgatgcggt tcaccagggt
      781 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
      841 ggtgcgctcc tggacgtagc
//
