LOCUS       sequence-386943-        9928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter: 
            p35S-PhiC31-DST-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P25
FEATURES             Location/Qualifiers
     source          1..9928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    257..279
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    287..463
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(695..1720)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1740..2085)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2288..2310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2302..2319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2303..2319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2326..2345
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2326..2344
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3342..3687
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3778..5592
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             5593..5613
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6305..6329
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6420..7008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6500..6519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8517..9332)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8517..9332)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9240..9259
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9324..9343
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9623..9928,1..130)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc gcccttggct
       61 tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc ggttgcccac
      121 cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt tataaatttt
      181 tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg gatttccgat
      241 ccccggaatt agatcttggc aggatatatt gtggtgtaac gttatcgatc tggattttag
      301 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
      361 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata agaaccctaa
      421 tttcccttat cgggaaacta ctcacacatt atttatggag aaaatagaga gagatagatt
      481 tgtagagaga gactggtgat ttcagcgtac cgaattaatt cctgaatcac tgcgaccggc
      541 cctcccgcga cccagccgag cgagcttagc gaactgtgga cgagaactgt gccaccaagc
      601 gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt
      661 ctcgaaatca gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
      721 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
      781 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
      841 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
      901 gttggtcaag accaatgcgg agcatatacg cccggagcct tggcgatcct gcaagctccg
      961 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     1021 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     1081 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     1141 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     1201 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     1261 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     1321 cgaacccgct cgtctggcta agatcggccg cagcgatagc atccatagcc tccgcgaccg
     1381 gctgaagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     1441 ggcgggagat gcaataggtc aggctctcgc tgaactcccc aatgtcaagc acttccggaa
     1501 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     1561 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     1621 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     1681 acttctcgac agacgtcgcg gtgagttcag gctttttcat aggggggatc agcttgggct
     1741 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     1801 gattgtgcgt catcccttac gtcagtggag atgtcacatc aatccacttg ctttgaagac
     1861 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     1921 ccactgtcgg cagaggcatc ttgaatgata gcctttcctt tatcgcaatg atggcatttg
     1981 taggagccac cttccttttc tactgtcctt tcgatgaagt gacagatagc tgggcaatgg
     2041 aatccgagga ggtttcccga aattaccctt tgttgaaaag tctcaatagc cctttggtct
     2101 tctgagactg tatctttgac atttttggag taggggtacg ataacattaa cgcttacaat
     2161 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     2221 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2281 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     2341 tagggcttgg aggaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
     2401 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
     2461 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
     2521 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
     2581 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
     2641 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
     2701 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
     2761 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
     2821 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     2881 ctgacccaca gatggttaga gaggcctacg cagcaagtct catcaagacg atctacccga
     2941 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     3001 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     3061 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     3121 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     3181 gaggatctaa cagaactcgc cgtcaagact ggcgaacagt tcatacagag tcttttacga
     3241 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     3301 aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt tcaacaaagg
     3361 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     3421 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     3481 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     3541 gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg tgacatctcc
     3601 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     3661 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctcattt ttacaacaat
     3721 taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat cgatacaatg
     3781 gacacgtatg ccggcgctta tgatagacaa tcaagagaaa gagagaactc ttctgccgcc
     3841 tctccagcta cacagagatc tgccaacgag gataaagctg ctgatttaca aagagaggta
     3901 gagagagatg ggggtaggtt tagatttgtg ggtcatttct ctgaggctcc tggtacatca
     3961 gcctttggta ctgctgaacg gcctgaattt gaaagaatat taaatgagtg tcgggccggt
     4021 cgactaaata tgataatagt ttatgatgta tctaggttca gtagactcaa agttatggat
     4081 gcaattccga tcgtgagcga gctacttgca ctcggtgtta ctatcgtaag tacacaagaa
     4141 ggtgtattca ggcagggtaa tgtgatggac cttatacacc ttattatgcg actcgacgct
     4201 agccataaag agtcatcact gaagagtgct aaaatcttgg acaccaagaa cctgcaaagg
     4261 gagcttggag gctatgtagg aggaaaagct ccgtatggtt tcgaactcgt gagtgagaca
     4321 aaagagatta ccagaaatgg taggatggtg aacgtggtta ttaataagct agctcatagc
     4381 acaacccctc tcacagggcc ttttgagttc gaacctgacg ttattagatg gtggtggaga
     4441 gagatcaaaa ctcacaaaca tcttccattt aagccaggta gccaggctgc tatccaccct
     4501 ggatctatca ctgggctttg taaaaggatg gatgctgacg cagtgccaac tcgaggggaa
     4561 acaatcggta agaagactgc atcttccgct tgggatcccg ctacagttat gagaatcctt
     4621 cgggatccgc gtattgctgg attcgccgca gaagtcattt ataagaaaaa acctgatggt
     4681 actcctacta caaaaatcga aggttatagg atccaaaggg atcctattac tttgcgtcca
     4741 gttgaattag actgtggacc gatcatcgaa cctgccgagt ggtacgagtt gcaggcctgg
     4801 cttgatggta gagggcgtgg aaagggttta tctcggggac aggcaatttt gtcagcaatg
     4861 gataagcttt attgtgagtg tggggctgtt atgacatcaa aacgtggaga agagtcaatc
     4921 aaggattctt acaggtgcag acgtcgtaaa gtggtggacc cttctgctcc aggacagcat
     4981 gagggtacat gcaacgtttc tatggctgct cttgataaat ttgtggccga acgtattttc
     5041 aacaagatta gacacgctga aggggatgag gaaactttag ctcttctttg ggaggctgcc
     5101 cggcgttttg gtaagctaac agaggcacca gaaaaaagtg gcgaaagagc taatttggta
     5161 gccgaaagag cagacgcctt gaatgcattg gaagaacttt acgaagatag ggctgccgga
     5221 gcatatgatg gaccagttgg tcggaagcat ttcaggaagc aacaagctgc actaactttg
     5281 agacagcaag gcgcagagga gcgactcgca gagcttgagg ccgccgaggc cccaaagctt
     5341 ccactcgatc aatggtttcc tgaggatgcc gacgctgatc ctacaggtcc aaagagctgg
     5401 tggggaagag ctagcgtaga cgataagaga gtgtttgtgg gtctatttgt tgacaagatt
     5461 gtagttacta agtccactac gggtcgagga cagggcactc ccatcgaaaa gagagccagt
     5521 attacctggg caaagcctcc aaccgatgat gatgaagacg atgcacaaga tggtaccgag
     5581 gatgtggcag caccaaaaaa aaagaggaag gtgtaagctt agtactatac tacaacattt
     5641 ccatattttt tttagattgt tagctaattt cccctggaga taattgtaaa ttgtttcaat
     5701 gagaggaata tacaatacat agatcgtaat tgatcaatgc gtatttgcat gttggtaaga
     5761 gactcttatc aagaatccca tctcttgctt gctttttttt gttgtcttcc ctttgatagg
     5821 gtttgttttt cttgtttcag tgactttcta tgttaaaaga taatgtcagt aaaaggattt
     5881 ggttttctat tattctgaat cgattacgga agattcttgc ttaattccaa tctatacaag
     5941 tatcgtgaaa taatgaccgt ttatgtgatt aggagacgtg tttcattaat aaaatataag
     6001 atcaatacat tgttagtagt gataaactat gtacaaattg tattgattgt aaaagaaaca
     6061 caataggttc cttttttcta caatatattg tgacagactc tctgttttaa cgaatgaatt
     6121 aaatttgtca acgaaaaaat taaagaatga tgtgatcact ttgcaccttg gttcatcttc
     6181 agaggcttct gcatgttcca tgcattgagg ctctctacgg tcacaggttg tgagccgttg
     6241 ttaaagacaa acaaatgcgc taccacagaa tcaggggata acgcaggaaa gaacatgaag
     6301 gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc
     6361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6841 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6901 ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7141 tcaatctaaa gtactgagag atcccctcat aatttcccca aagcgtaacc atgtgtgaat
     7201 aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat tgtgtagcca
     7261 gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg atttctgact
     7321 gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc cgcaagccaa
     7381 catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc tgagctgctc
     7441 gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg gaaggtttct
     7501 tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg aatttcaaca
     7561 ggtaagttag ttgctagaac ccatggctcc tttgccgacg ctgagtagat tttaggtgac
     7621 gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga gcgagattta
     7681 tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata gcctaaagtc
     7741 tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt ttccgctcct
     7801 aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc cagcttctca
     7861 acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg tgtggtgtta
     7921 ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt tgaacattgc
     7981 tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag tgcgactgaa
     8041 gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac aagtacaatg
     8101 ggcatcgtat tgcccgaaca gataaagcta gcatgccaac ggtatacagc gagtcgctct
     8161 ttgtggaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg ttttgttctc
     8221 gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca gtcaagtaat
     8281 gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaattcggg gcagaattgg
     8341 taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta ttgatttttc
     8401 gcgaaaccat ttgatcatat gacaagatgt gtatccacct taacttaatg atttttacca
     8461 aaatcattag gggattcatc agtctaaagt atatatgtgt aacattggtc tagtgattag
     8521 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     8581 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     8641 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     8701 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     8761 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     8821 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     8881 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     8941 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     9001 tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca tgcatcatca
     9061 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     9121 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     9181 tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg cccgacatta
     9241 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctt
     9301 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     9361 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     9421 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     9481 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
     9541 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat
     9601 gatggggcga ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc ttttttatcc
     9661 ccggaagcct gtggatagag ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc
     9721 ttgattcacg gggctttccg gcccgctcca aaaactatcc acgtgaaatc gctaatcagg
     9781 gtacgtgaaa tcgctaatcg gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta
     9841 atcaaaaagg cacgtgagaa cgctaatagc cctttcagat caacagcttg caaacacccc
     9901 tcgctccggc aagtagttac agcaagta
//
