LOCUS       sequence-386947-        3268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: AtNLP6 promoter 1kb upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW0CM0020
FEATURES             Location/Qualifiers
     source          1..3268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(57..151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(172..831)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     746..765
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     993..1011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1015..1058)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1061..1082
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2302..2359
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2383..2400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2554..3142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2634..2653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
       61 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      121 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      181 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      241 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      301 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      361 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa
      421 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      481 tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg
      541 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      601 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      661 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      721 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      781 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      841 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      901 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
      961 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     1021 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag
     1081 agcgatgagt ggtctcggga gctcatcaac ttgaaacaag tggggattta cgttgcaagc
     1141 atttcttctc cttggggtta aaatattgat taacagctaa ataacaaatt agggaagtta
     1201 ttataacatt aaaagaaaga ataatcacga ttagatgtcc ccactaggaa aacaagttac
     1261 tgttaataaa aattttatgt attgcaaaag ggcttaagta aggatccact ttgggtaagt
     1321 agttgaaaat ggctgagtaa gaataatcac gattagatgt cctcgctttt ttattcatct
     1381 cccaaagcga gattcgtttt tttcattgtc gtcaattatt gcattgttta tcataattat
     1441 ttgctaagaa catcattatt attgaataaa ttatagtagt cgttgttatt ctactcaccc
     1501 acaacacgca cttagtgcct aattacgtct taatttaata ttttttaaag cttttaggcg
     1561 cataaaatat gctatgacaa ctttgttcat ggtggtggac gtgtaagaaa cgtctaaaga
     1621 tctactctat ataacataat caattcgttt tttgaaatgt gataaaaaca aacctttacc
     1681 atatattcgc ctctggcaga aacaatgcat ttggaataaa atagaatcgt atcacagcat
     1741 ttaaaaacga ttattcaaat agtctaatag tatttattca cacctttatg ttttatgtat
     1801 atcgaatttt gtcaaagcca atattctaga gatggtcaat caatatcaat acagtttttt
     1861 tttttggcca atgttctaat attcaagttt gttagtatta atagaaaaac aaaatttcga
     1921 tgccttttgt ctcataaaaa tattaaagaa aataatacaa agaataaaga atttgcatga
     1981 aatgggagat attctagtgg ttggggtaaa atggtacagc gtggaatcaa atgtcttcaa
     2041 caaaatcaaa ttattttatc tcaaaaagat ttaaatccca atttaaataa tattacaact
     2101 tgtcttctcc tcttatttct tctctctctc tttctgttga agaagctgaa tcagaagagt
     2161 ttttttttgc ttcaaaagga gctccggcga aacttccgga gatattaaca cttactctga
     2221 cgagtttttg tctcgtctta cgtctttgct gctgctgttg aatgtgagac cactcatcgc
     2281 gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     2341 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     2401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2521 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     2581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3241 ctagatcctt ttaaattaaa aatgaagt
//
