LOCUS       sequence-386949-        2205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part: MinSyn_305.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPYC0CM0522
FEATURES             Location/Qualifiers
     source          1..2205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     258..277
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     505..523
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(527..570)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      751..808
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(832..849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1003..1591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1083..1102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1774..1868)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1889..2205,1..343))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
       61 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
      121 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
      181 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
      241 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
      301 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
      361 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
      421 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt
      481 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
      541 aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc
      601 gttttagtga ggcttacgtc tgggattacg tctacttacg tctgggatta cgtctactga
      661 cgtaagggat gacgtaaact atataaggtt ttgctattca ttgaaagcag tagtgactga
      721 tttgtatata atgtactcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc
      781 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
      841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
     1021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1741 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg
     1801 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     1861 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
     1921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     1981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     2041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     2101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     2161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaact
//
