LOCUS       sequence-38695-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Cowley SC, Av-Gay Y
  TITLE     Monitoring promoter activity and protein localization in 
            Mycobacterium spp. using green fluorescent protein.
  JOURNAL   Gene. 2001 Feb 21;264(2):225-31.
  PUBMED    11250077
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-02-21"; volume: "264(2)"; pages: "225-31"
COMMENT     Sequence Label: pSC301
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(313..329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(313..329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(326..348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    337..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(361..367,368..385,386..391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    406..427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(544..561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(795..814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcctttact catatgtttt tcctccttat aaagttaatc agaattagct tgatatcgaa
       61 ttcctgcagc ccgggggatc ccacggcatt ccttccttcg atgaaatgat cgtccgatgt
      121 ttcttttcgc gcgggtgcct cccacgcgat gggctggctc tagcttgaca agtcaaccct
      181 gctccatgac cgctcgcgtc gcaacaacga ccttggggtc gatgccccag ggcggacggc
      241 ataggctacc aggacgcccc caggctgtct agagcggccg ccaccgcggt gcgagccaga
      301 tctggtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      361 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      421 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      841 tgttccgacc ctgccgctta ccg
//
