LOCUS       sequence-386950-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 gene expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ1KN0148
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(370..392)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    478..618
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     533..552
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(633..650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(804..1392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(884..903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1480..2295)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1480..2295)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2203..2222
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2728..2752
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3183..3528
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3471..3491
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     regulatory      3999..4008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6431..6605
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6626..6650
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7951..8580
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9009..10082
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      join(10148..10208,1..134)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
       61 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
      121 ctggcctgtg cataactgtc tggccagcgc acagccgaag tgctgcaaaa agcgcctacc
      181 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
      241 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
      301 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
      361 cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga
      421 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
      481 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
      541 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      601 agaaaatacc gcatcaggcg ctcatccgct tcctcgctca ctgactcgct gcgctcggtc
      661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1201 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1441 aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact catcgagcat
     1501 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     1561 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     1621 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1681 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1741 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1801 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac
     1861 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1921 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1981 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     2041 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     2101 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     2161 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     2221 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     2281 ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag tatttaaaga
     2341 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     2401 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     2461 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     2521 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     2581 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     2641 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     2701 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acataacgaa
     2761 ttgctcttca gcaaggaggt caacatggtg gagcacgaca ctctggtcta ctccaaaaat
     2821 gtcaaagata cagtctcaga agatcaaagg gctattgaga cttttcaaca aaggataatt
     2881 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
     2941 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
     3001 gatctctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     3061 aaagaagagg ttccaaccac gtctacaaag caagtggatt gatgtgataa catggtggag
     3121 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     3181 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     3241 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     3301 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     3361 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     3421 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     3481 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     3541 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     3601 tttacaatta tcgatacaat ggcaaatcgc ggaggtgaat atctgtacga tgagttatgg
     3661 aaattatgcg cgggacctct tgttgatgtt cctcaagctc aagaaagagt ttattatttt
     3721 cctcaaggtc acatggaaca actcgaagcg tcaacgcaac aagtcgactt aaatacgatg
     3781 aagcctcttt ttgttcttcc tcctaagatt ctctgcaatg ttatgaacgt tagtcttcag
     3841 gcggagaaag atacggatga ggtctatgct cagattactt tgatccctgt tggaactgaa
     3901 gttgatgaac ctatgagtcc tgatccctct cctcctgagt tgcaaaggcc gaaagttcac
     3961 tctttcagca aggttttgac agcgtctgat acaagcaccc atggtggctt ttctgttcta
     4021 aggaaacatg ccacggaatg tcttcctccg ctggatatga ctcagcaaac cccgacccag
     4081 gagttagtag ccgaagatgt gcacggttat cagtggaaat tcaagcatat ttttagaggc
     4141 caaccacgga ggcatctatt gacgacaggg tggagcacct ttgttacatc aaagagattg
     4201 gttgctgggg acacctttgt attcctgaga ggggagaacg gagagttgcg agttggagtc
     4261 agacgtgcta atcttcaaca gagcagtatg ccttcatccg ttatatcaag tcatagtatg
     4321 catctgggag tgcttgctac tgcacgccat gctactcaaa cgaaaactat gttcattgta
     4381 tactataaac caaggacaag ccaattcatc attagcttga acaaatatct agaagccatg
     4441 agcaataagt tctctgtagg gatgagattt aagatgcgtt ttgagggaga ggattcccct
     4501 gaaagaagat attctggcac ggttattggt gtgaaagact gctcccctca ctggaaagac
     4561 tcaaaatggc gatgcttaga agttcattgg gatgagcctg catcgatttc aagacccaat
     4621 aaggtttcac catgggaaat tgaaccgttt gtaaattcag aaaacgttcc caaatcagtt
     4681 atgctgaaga acaaaaggcc ccgtcaagtt agtgaagtat ctgcacttga tgtaggcata
     4741 acagcttcaa acctttggag ctctgttttg acgcaacccc atgagtttgc acaatcgtgc
     4801 atcacctcac agtggagttc tcctcagcaa tgtcatcgtg atgcaaatga ggatgctaag
     4861 aaatctgact ggctaaataa ctcttactct gtgtcaaatg tagcaaaaga ctcaacactg
     4921 aacgaccaaa tggtttcccc agtcgagcag aagaagcctg agacaaccgc taattataga
     4981 ttatttggaa ttgatctgat gagttcctcc ctagcggttc ctgaggagaa aactgcaccc
     5041 atgcgaccaa tcaacatatc caaaccgact atggacagcc actcagaccc aaaatcagag
     5101 atttcaaaag tatcagaaga gaaaaagcag gaacctgcgg agggatcacc aaaagaggtc
     5161 caaagcaagc aaagcagttc tacaagaagc cgtaccaagg tgcagatgca aggcgtacct
     5221 gtgggcaggg ctgtggattt aaatgcgcta aaggggtaca acgagctcat agatgacatt
     5281 gagaagctgt ttgacataaa aggggaactg cggagtcgca atcaatggga aatagtgttc
     5341 acagacgatg agggagatat gatgcttgtc ggtgatgacc catggcctga gttctgcaac
     5401 atggtgaaga gaatattcat atggtcgaaa gaggaagtga agaaaatgac gcctgggaac
     5461 caactccgga tgctgttaag ggaagttgaa acaacactaa caacaacttc caaaacagat
     5521 aatcattcca acggttcggg cgggagcggt ggaagcggcg aaattcagca agccactgca
     5581 ggagtctcac aagacacttc ggaaaatcct aacaaaacaa tagttcctgc agcattacca
     5641 cagctcaccc ctaccttggt gtcactgctg gaggtgattg aacccgaggt gttgtatgca
     5701 ggatatgata gctctgttcc agattcagca tggagaatta tgaccacact caacatgtta
     5761 ggtgggcgtc aagtgattgc agcagtgaaa tgggcaaagg cgatactagg cttgagaaac
     5821 ttacacctcg atgaccaaat gaccctgcta cagtactcat ggatgtttct catggcattt
     5881 gccttgggtt ggagatcata cagacaatca agcggaaacc tgctctgctt tgctcctgat
     5941 ctgattatta atgagcagag aatgtctcta ccctgcatgt atgaccaatg taaacacatg
     6001 ctgtttgtct cctctgaatt acaaagattg caggtatcct atgaagagta tctctgtatg
     6061 aaaaccttac tgcttctctc ctcagttcct aaggaaggtc tgaagagtca agagttattt
     6121 gatgagattc gaatgactta tatcaaagag ctaggaaaag ccatcgtcaa aagggaaggg
     6181 aactccagtc agaactggca acggttttac caactgacaa agcttctgga ctccatgcat
     6241 gaggtggttg agaatctcct tacctactgc ttccagacat ttttggataa gaccatgagt
     6301 attgaattcc cagagatgtt agctgaaatc atcactaatc agataccaaa atattcaaat
     6361 ggaaatatca aaaagcttct gtttcatcaa aaatgagctt ctctagctag agtcgatcga
     6421 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
     6481 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     6541 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     6601 agatcgctat actgaagagc aattctgaca ggatatattg gcgggtaaac ctaagagaaa
     6661 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     6721 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     6781 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg
     6841 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     6901 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     6961 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     7021 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     7081 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     7141 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     7201 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     7261 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     7321 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     7381 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     7441 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     7501 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     7561 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     7621 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     7681 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     7741 gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     7801 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     7861 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     7921 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     7981 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     8041 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     8101 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     8161 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     8221 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     8281 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     8341 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     8401 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     8461 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     8521 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     8581 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     8641 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     8701 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     8761 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     8821 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     8881 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     8941 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     9001 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     9061 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     9121 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     9181 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     9241 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     9301 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     9361 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     9421 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     9481 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     9541 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     9601 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     9661 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     9721 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     9781 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     9841 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     9901 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     9961 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    10021 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    10081 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
    10141 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    10201 cgtacatt
//
