LOCUS       sequence-386953-       10100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 gene expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ1KN0147
FEATURES             Location/Qualifiers
     source          1..10100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(375..397)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    483..623
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     538..557
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(638..655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(809..1397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(889..908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1485..2300)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1485..2300)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2208..2227
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2733..2757
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3188..3533
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3476..3496
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    6328..6502
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6523..6547
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7848..8477
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8906..9979
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      join(10045..10100,1..139)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
       61 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
      121 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagtgctg caaaaagcgc
      181 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
      241 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
      301 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
      361 gatgacggtg aaaacctctg acacatgcag ctcccggtga cggtcacagc ttgtctgtaa
      421 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      481 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
      541 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
      601 taaggagaaa ataccgcatc aggcgctcat ccgcttcctc gctcactgac tcgctgcgct
      661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1441 acgaaaactc acgttaaggg attttggtca tgcattctag gtgattagaa aaactcatcg
     1501 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     1561 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     1621 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1681 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1741 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1801 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga
     1861 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1921 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1981 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2041 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2101 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     2161 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
     2221 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
     2281 tcccgttgaa tatggctcat aacagaactt attatttcct tcctcttttc tacagtattt
     2341 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
     2401 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
     2461 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
     2521 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     2581 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     2641 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     2701 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacata
     2761 acgaattgct cttcagcaag gaggtcaaca tggtggagca cgacactctg gtctactcca
     2821 aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt caacaaagga
     2881 taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     2941 cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
     3001 ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     3061 tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt gataacatgg
     3121 tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa
     3181 gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc
     3241 cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc
     3301 atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag
     3361 atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa
     3421 agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa tcccactatc
     3481 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctcga
     3541 gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
     3601 ttacatttac aattatcgat acaatggcga gtgttgaagg tgatgatgat ttcggaagtt
     3661 cttcgtcaag gtcttatcaa gatcaactat acacagagct atggaaagtt tgtgcaggtc
     3721 cattagtgga agttcctcgt gctcaagaga gagttttcta cttccctcag ggtcacatgg
     3781 aacaacttgt ggcgtcaact aatcaaggaa tcaattcaga agaaatacct gtttttgatc
     3841 ttcctccaaa gatactttgt cgagttcttg atgtcacttt aaaggcggag catgaaacag
     3901 atgaggttta cgctcagatc acattacaac cagaggaaga tcaaagtgaa ccaacaagtc
     3961 ttgatccacc tattgttgga ccaactaagc aagagtttca ttcgtttgtt aagattttaa
     4021 cggcttcaga tacaagcact catggtggat tctctgttct tcgtaaacac gccactgaat
     4081 gcttgccttc tttggatatg acacaagcta ctcctactca agaacttgtg actagagatc
     4141 ttcatggctt tgaatggagg tttaagcata tattcagagg acaaccacgg aggcatttgc
     4201 ttactacggg ttggagtaca tttgtatcct cgaaaagact tgtagctgga gatgcttttg
     4261 tgttcttgag gggtgagaat ggggatttac gggttggagt gagacgatta gctcggcatc
     4321 aaagcacaat gcctacttcg gttatttcaa gtcagagcat gcatttggga gttcttgcta
     4381 cagcttctca tgctgtgcgt acaacaacaa tctttgttgt cttttacaag cctaggataa
     4441 gccaattcat agttggggtg aacaagtata tggaagctat aaagcatgga ttttctctcg
     4501 gtacccgatt cagaatgagg tttgaaggag aagagtctcc tgagagaata tttactggta
     4561 cgattgtggg aagtggagat ctatcttcac aatggccagc ttctaaatgg aggtcattgc
     4621 aggtacaatg ggatgagcca acaacagttc aaagaccaga taaagtctca ccatgggaga
     4681 tagagccttt cttggcaact tccccaattt caactcctgc tcaacaacca caatcgaaat
     4741 gcaagcggtc aagacccatc gagccatcag ttaaaacacc agccccacct agtttcttgt
     4801 acagcctccc tcagagccaa gattccatta atgcatccct taaactgttt caagatccat
     4861 cacttgagag aatttcaggt ggatactcct caaacaacag cttcaaaccc gagactcctc
     4921 ctcctccaac gaattgtagc tataggttgt ttggatttga tctcacaagc aattctcctg
     4981 ctccaatccc tcaagacaag caaccgatgg atacttgtgg agctgccaag tgtcaagaac
     5041 ccatcactcc aacctcaatg agtgagcaga agaagcaaca aacatcaaga agtcgaacta
     5101 aagtgcaaat gcaaggcatt gcggttggtc gtgcggttga tttaacactg ttgaaatctt
     5161 acgatgaact gattgatgag cttgaggaga tgtttgagat tcaaggacag cttcttgccc
     5221 gagacaaatg gatcgttgtc ttcactgatg atgaaggaga tatgatgctt gctggtgatg
     5281 atccgtggaa tgagttttgc aagatggcaa agaagatatt tatatattcg agcgatgagg
     5341 ttaagaaaat gacaacgaaa ctgaagattt cttcgtcgtt agagaatgag gaatatggta
     5401 atgaatcatt cgaaaatcgt agtagggggg gttcgggcgg gagcggtgga agcggcgaaa
     5461 ttcagcaagc cactgcagga gtctcacaag acacttcgga aaatcctaac aaaacaatag
     5521 ttcctgcagc attaccacag ctcaccccta ccttggtgtc actgctggag gtgattgaac
     5581 ccgaggtgtt gtatgcagga tatgatagct ctgttccaga ttcagcatgg agaattatga
     5641 ccacactcaa catgttaggt gggcgtcaag tgattgcagc agtgaaatgg gcaaaggcga
     5701 tactaggctt gagaaactta cacctcgatg accaaatgac cctgctacag tactcatgga
     5761 tgtttctcat ggcatttgcc ttgggttgga gatcatacag acaatcaagc ggaaacctgc
     5821 tctgctttgc tcctgatctg attattaatg agcagagaat gtctctaccc tgcatgtatg
     5881 accaatgtaa acacatgctg tttgtctcct ctgaattaca aagattgcag gtatcctatg
     5941 aagagtatct ctgtatgaaa accttactgc ttctctcctc agttcctaag gaaggtctga
     6001 agagtcaaga gttatttgat gagattcgaa tgacttatat caaagagcta ggaaaagcca
     6061 tcgtcaaaag ggaagggaac tccagtcaga actggcaacg gttttaccaa ctgacaaagc
     6121 ttctggactc catgcatgag gtggttgaga atctccttac ctactgcttc cagacatttt
     6181 tggataagac catgagtatt gaattcccag agatgttagc tgaaatcatc actaatcaga
     6241 taccaaaata ttcaaatgga aatatcaaaa agcttctgtt tcatcaaaaa tgagcttctc
     6301 tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     6361 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     6421 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     6481 atccagtact aaaatccaga tcgctatact gaagagcaat tctgacagga tatattggcg
     6541 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     6601 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     6661 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     6721 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     6781 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     6841 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     6901 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     6961 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     7021 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     7081 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     7141 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     7201 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     7261 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     7321 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     7381 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     7441 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     7501 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     7561 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     7621 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc
     7681 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     7741 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     7801 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     7861 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     7921 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     7981 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     8041 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     8101 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     8161 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     8221 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     8281 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     8341 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     8401 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     8461 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     8521 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     8581 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     8641 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     8701 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     8761 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     8821 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg
     8881 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     8941 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     9001 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     9061 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     9121 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     9181 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     9241 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     9301 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     9361 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     9421 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     9481 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     9541 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     9601 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     9661 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     9721 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     9781 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     9841 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     9901 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     9961 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
    10021 aaaaggtcga aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta
    10081 cattgggaac cggaacccgt
//
