LOCUS       sequence-386954-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS 
            tropism in mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, 
            Solomon IH, Tabebordbar M, Sabeti PC
  TITLE     Systemic administration of novel engineered AAV capsids facilitates 
            enhanced transgene expression in the macaque CNS.
  JOURNAL   Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 
            10.1016/j.medj.2022.11.002.
  PUBMED    36417917
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Med (N Y). 
            2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002."
COMMENT     Sequence Label: kiCAP-AAV-MDV1A
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2776..2886
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2925..2943)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2926..2943)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2963..2980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2969..3193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3255..3273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3292..3310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3329..3347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3366..3384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3403..3421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3440..3458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3477..3495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3514..3532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6249..6260
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6601..6621)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6601..6619)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6640..6656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6640..6656)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6653..6675)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6664..6680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6688..6694,6695..6712,6713..6718))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6733..6754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6871..6888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7042..7630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7122..7141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7801..8610)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8524..8543
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8611..8715)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8741..9196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8878..8899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9090..9109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9323..9345
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9337..9354
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9338..9354
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9364..9383
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9364..9382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccccccctc gatcgaggtc gacacgcgtg gaggggtgga gtcgtgacgt gaattacgtc
       61 atagggttag ggaggtcctg tattagaggt cacgtgagtg ttttgcgaca ttttgcgaca
      121 ccatgtggtc acgctgggta tttaagcccg agtgagcacg cagggtctcc attttgaagc
      181 gggaggtttg aacgcgcagc cgccatgccg gggttttacg agattgtgat taaggtcccc
      241 agcgaccttg acgagcatct gcccggcatt tctgacagct ttgtgaactg ggtggccgag
      301 aaggaatggg agttgccgcc agattctgac atggatctga atctgattga gcaggcaccc
      361 ctgaccgtgg ccgagaagct gcagcgcgac tttctgacgg aatggcgccg tgtgagtaag
      421 gccccggagg ctcttttctt tgtgcaattt gagaagggag agagctactt ccacatgcac
      481 gtgctcgtgg aaaccaccgg ggtgaaatcc atggttttgg gacgtttcct gagtcagatt
      541 cgcgaaaaac tgattcagag aatttaccgc gggatcgagc cgactttgcc aaactggttc
      601 gcggtcacaa agaccagaaa tggcgccgga ggcgggaaca aggtggtgga tgagtgctac
      661 atccccaatt acttgctccc caaaacccag cctgagctcc agtgggcgtg gactaatatg
      721 gaacagtatt taagcgcctg tttgaatctc acggagcgta aacggttggt ggcgcagcat
      781 ctgacgcacg tgtcgcagac gcaggagcag aacaaagaga atcagaatcc caattctgat
      841 gcgccggtga tcagatcaaa aacttcagcc aggtacatgg agctggtcgg gtggctcgtg
      901 gacaagggga ttacctcgga gaagcagtgg atccaggagg accaggcctc atacatctcc
      961 ttcaatgcgg cctccaactc gcggtcccaa atcaaggctg ccttggacaa tgcgggaaag
     1021 attatgagcc tgactaaaac cgcccccgac tacctggtgg gccagcagcc cgtggaggac
     1081 atttccagca atcggattta taaaattttg gaactaaacg ggtacgatcc ccaatatgcg
     1141 gcttccgtct ttctgggatg ggccacgaaa aagttcggca agaggaacac catctggctg
     1201 tttgggcctg caactaccgg gaagaccaac atcgcggagg ccatagccca cactgtgccc
     1261 ttctacgggt gcgtaaactg gaccaatgag aactttccct tcaacgactg tgtcgacaag
     1321 atggtgatct ggtgggagga ggggaagatg accgccaagg tcgtggagtc ggccaaagcc
     1381 attctcggag gaagcaaggt gcgcgtggac cagaaatgca agtcctcggc ccagatagac
     1441 ccgactcccg tgatcgtcac ctccaacacc aacatgtgcg ccgtgattga cgggaactca
     1501 acgaccttcg aacaccagca gccgttgcaa gaccggatgt tcaaatttga actcacccgc
     1561 cgtctggatc atgactttgg gaaggtcacc aagcaggaag tcaaagactt tttccggtgg
     1621 gcaaaggatc acgtggttga ggtggagcat gaattctacg tcaaaaaggg tggagccaag
     1681 aaaagacccg cccccagtga cgcagatata agtgagccca aacgggtgcg cgagtcagtt
     1741 gcgcagccat cgacgtcaga cgcggaagct tcgatcaact acgcggacag gtaccaaaac
     1801 aaatgttctc gtcacgtggg catgaatctg atgctgtttc cctgcagaca atgcgagaga
     1861 ctgaatcaga attcaaatat ctgcttcact cacggtgtca aagactgttt agagtgcttt
     1921 cccgtgtcag aatctcaacc cgtttctgtc gtcaaaaagg cgtatcagaa actgtgctac
     1981 attcatcaca tcatgggaaa ggtgccagac gcttgcactg cttgcgacct ggtcaatgtg
     2041 gacttggatg actgtgtttc tgaacaataa atgacttaaa ccaggtattg ctgccgatgg
     2101 ttatcttcca gattggctcg aggacaacct tagtgaacta gtgccaccat gagtcggctg
     2161 gataaatcta aagtcataaa cggcgctctg gaattactca atgaagtcgg tatcgaaggc
     2221 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     2281 gtgaagaaca agcgggccct gctcgatgcc ctggccatcg agatgctgga caggcatcat
     2341 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     2401 tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     2461 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     2521 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     2581 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     2641 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     2701 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     2761 agcggcgggc cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     2821 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     2881 atgctccccg ggtaaatgca tgaattcgat ctagagggcc ctattctata gtgtcaccta
     2941 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3001 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3061 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3121 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3181 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgaatca agctatcaag
     3241 tgccacctga cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca
     3301 gtgatagaga aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct
     3361 cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag
     3421 aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc
     3481 tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc
     3541 cctatataag cagagagatc tgttcaaatt tgaactgact aagcggctcc cgccagattt
     3601 tggcaagatt actaagcagg aagtcaagga cttttttgct tgggcaaagg tcaatcaggt
     3661 gccggtgact cacgagttta aagttcccag ggaattggcg ggaactaaag gggcggagaa
     3721 atctctaaaa cgcccactgg gtgacgtcac caatactagc tataaaagtc tggagaagcg
     3781 ggccaggctc tcatttgttc ccgagacgcc tcgcagttca gacgtgactg ttgatcccgc
     3841 tcctctgcga ccgctagctt cgatcaacta cgcggacagg taccaaaaca aatgttctcg
     3901 tcacgtgggc atgaatctga tgctgtttcc ctgcagacaa tgcgagagac tgaatcagaa
     3961 ttcaaatatc tgcttcactc acggtgtcaa agactgttta gagtgctttc ccgtgtcaga
     4021 atctcaaccc gtttctgtcg tcaaaaaggc gtatcagaaa ctgtgctaca ttcatcacat
     4081 catgggaaag gtgccagacg cttgcactgc ttgcgacctg gtcaatgtgg acttggatga
     4141 ctgtgtttct gaacaataaa tgacttaaac caggtatggc tgccgatggt tatcttccag
     4201 attggctcga ggacaacctt agtgaaggaa ttcgcgagtg gtgggctttg aaacctggag
     4261 cccctcaacc caaggcaaat caacaacatc aagacaacgc tcgaggtctt gtgcttccgg
     4321 gttacaaata ccttggaccc ggcaacggac tcgacaaggg ggagccggtc aacgcagcag
     4381 acgcggcggc cctcgagcac gacaaggcct acgaccagca gctcaaggcc ggagacaacc
     4441 cgtacctcaa gtacaaccac gccgacgccg agttccagga gcggctcaaa gaagatacgt
     4501 cttttggggg caacctcggg cgagcagtct tccaggccaa aaagaggctt cttgaacctc
     4561 ttggtctggt tgaggaagcg gctaagacgg ctcctggaaa gaagaggcct gtagagcagt
     4621 ctcctcagga accggactcc tccgcgggta ttggcaaatc gggtgcacag cccgctaaaa
     4681 agagactcaa tttcggtcag actggcgaca cagagtcagt cccagaccct caaccaatcg
     4741 gagaacctcc cgcagccccc tcaggtgtgg gatctcttac aatggcttca ggtggtggcg
     4801 caccagtggc agacaataac gaaggtgccg atggagtggg tagttcctcg ggaaattggc
     4861 attgcgattc ccaatggctg ggggacagag tcatcaccac cagcacccga acctgggccc
     4921 tgcccaccta caacaatcac ctctacaagc aaatctccaa cagcacatct ggaggatctt
     4981 caaatgacaa cgcctacttc ggctacagca ccccctgggg gtattttgac ttcaacagat
     5041 tccactgcca cttctcacca cgtgactggc agcgactcat caacaacaac tggggattcc
     5101 ggcctaagcg actcaacttc aagctcttca acattcaggt caaagaggtt acggacaaca
     5161 atggagtcaa gaccatcgcc aataacctta ccagcacggt ccaggtcttc acggactcag
     5221 actatcagct cccgtacgtg ctcgggtcgg ctcacgaggg ctgcctcccg ccgttcccag
     5281 cggacgtttt catgattcct cagtacgggt atctgacgct taatgatgga agccaggccg
     5341 tgggtcgttc gtccttttac tgcctggaat atttcccgtc gcaaatgcta agaacgggta
     5401 acaacttcca gttcagctac gagtttgaga acgtaccttt ccatagcagc tacgctcaca
     5461 gccaaagcct ggaccgacta atgaatccac tcatcgacca atacttgtac tatctctcaa
     5521 agactattaa cggttctgga cagaatcaac aaacgctaaa attcagtgtg gccggaccca
     5581 gcaacatggc tgtccaggga agaaactaca tacctggacc cagctaccga caacaacgtg
     5641 tctcaaccac tgtgactcaa aacaacaaca gcgaatttgc ttggcctgga gcttcttctt
     5701 gggctctcaa tggacgtaat agcttgatga atcctggacc tgctatggcc agccacaaag
     5761 aaggagagga ccgtttcttt cctttgtctg gatctttaat ttttggcaaa caaggaactg
     5821 gaagagacaa cgtggatgcg gacaaagtca tgataaccaa cgaagaagaa attaaaacta
     5881 ctaacccggt agcaacggag tcctatggac aagtggccac aaaccaccag agtgcccaac
     5941 ggagtgttgg gagtgtgtat gcacaggcgc agaccggctg ggttcaaaac caaggaatac
     6001 ttccgggtat ggtttggcag gacagagatg tgtacctgca aggacccatt tgggccaaaa
     6061 ttcctcacac ggacggcaac tttcaccctt ctccgctgat gggagggttt ggaatgaagc
     6121 acccgcctcc tcagatcctc atcaaaaaca cacctgtacc tgcggatcct ccaacggcct
     6181 tcaacaagga caagctgaac tctttcatca cccagtattc tactggccaa gtcagcgtgg
     6241 agatcgagtg ggagctgcag aaggaaaaca gcaagcgctg gaacccggag atccagtaca
     6301 cttccaacta ttacaagtct aataatgttg aatttgctgt taatactgaa ggtgtatata
     6361 gtgaaccccg ccccattggc accagatacc tgactcgtaa tctgtaattg cttgttaatc
     6421 aataaaccgt ttaattcgtt tcagttgaac tttggtctct gcgtatttct ttcttatcta
     6481 gtttccatgg ctacgtagat aagtagcatg gcgggttaat cattaactac agatatccat
     6541 cacactggcg gccgctcgac tagagcggcc gccaccgcgg tggagctcca gcttttgttc
     6601 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7801 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     7861 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     7921 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     7981 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     8041 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     8101 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     8161 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     8221 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     8281 ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc
     8341 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     8401 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     8461 caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac
     8521 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     8581 cctcgacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat
     8641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     8761 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     8821 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     8881 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8941 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     9001 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     9061 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     9121 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     9181 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     9241 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     9301 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     9361 gcgtaatacg actcactata gggcgaattg ggtaccgg
//
