LOCUS       sequence-386955-       11171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 gene expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ1KN0143
FEATURES             Location/Qualifiers
     source          1..11171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(371..393)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    479..619
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     534..553
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(634..651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(805..1393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(885..904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1481..2296)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1481..2296)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2204..2223
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2729..2753
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3184..3529
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3472..3492
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    7395..7569
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7590..7614
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8915..9544
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9973..11046
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      join(11112..11171,1..135)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
       61 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      121 cctggcctgt gcataactgt ctggccagcg cacagccgaa gtgctgcaaa aagcgcctac
      181 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      241 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
      301 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
      361 acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg
      421 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      481 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
      541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
      601 gagaaaatac cgcatcaggc gctcatccgc ttcctcgctc actgactcgc tgcgctcggt
      661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     1261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1441 aaactcacgt taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca
     1501 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1561 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1621 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1681 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1741 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1801 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata
     1861 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1921 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1981 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     2041 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     2101 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     2161 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     2221 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
     2281 gttgaatatg gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag
     2341 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
     2401 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
     2461 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
     2521 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
     2581 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     2641 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     2701 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacataacga
     2761 attgctcttc agcaaggagg tcaacatggt ggagcacgac actctggtct actccaaaaa
     2821 tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac aaaggataat
     2881 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
     2941 agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
     3001 agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     3061 aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgata acatggtgga
     3121 gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc
     3181 tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc
     3241 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     3301 ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg
     3361 acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca
     3421 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
     3481 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctcgagtat
     3541 aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac
     3601 atttacaatt atcgatacaa tgtgcgagcc cgatgataat tccgctagaa acggcgtcac
     3661 tactcaacct tcgaggtcaa gggagcttct aatggatgtt gacgacttag atcttgacgg
     3721 ttcatggcca ctagatcaaa tcccttactt atcctcatcg aatcgcatga tttctccgat
     3781 ttttgtctcc tcttcctctg agcagccttg ctcgcctctc tgggctttct ccgacggtgg
     3841 aggaaatggt tttcaccacg caacctccgg tggcgatgat gagaagatca gctctgtctc
     3901 cggtgttcct tctttccgtc tcgccgagta tcctctcttc ctcccttact cttctccatc
     3961 agcagctgag aacacaacag agaagcataa cagtttccag tttccgtctc cattgatgag
     4021 cctagtccca ccagagaaca cagacaacta ctgtgtgatc aaagagagga tgactcaggc
     4081 gcttcgatac ttcaaagaat caaccgaaca acacgttttg gctcaggtct gggctcctgt
     4141 gagaaagaat ggtcgtgatt tgctgacgac tttgggtcaa ccttttgttc ttaatcctaa
     4201 tggtaatggg cttaatcaat acaggatgat ctctctcaca tatatgtttt ctgtggatag
     4261 tgaaagtgac gtagagctcg gactcccggg tcgagttttc cgtcagaaat tgcctgaatg
     4321 gactccaaat gttcagtact attccagcaa agaattctcg cggcttgatc acgccttgca
     4381 ctacaacgtg cgtggtacac tggccttgcc tgtctttaat ccctctggtc agtcctgcat
     4441 aggtgttgtg gaacttataa tgacctcaga gaagattcac tatgcacccg aagtggacaa
     4501 agtttgcaaa gcccttgagg cggtaaatct gaaaagctcg gaaatacttg atcaccaaac
     4561 aacacagata tgcaatgaga gtcgccaaaa cgcgcttgct gagattctcg aagtgctgac
     4621 agttgtatgt gaaacccata acttgcctct cgctcagact tgggttccat gtcagcatgg
     4681 gagcgttctt gccaatggtg gcggtctaaa gaaaaactgc accagctttg acggtagctg
     4741 catgggtcaa atctgcatgt ctacaaccga catggcctgc tatgtcgtgg atgctcatgt
     4801 ctggggcttt agagatgcct gtcttgaaca ccatctccag aaaggccagg gagtcgctgg
     4861 acgagctttt ctcaatggtg gctcatgttt ctgcagagac atcaccaagt tctgcaaaac
     4921 gcagtaccca ctagtccatt atgcgctcat gttcaagttg accacttgtt ttgcaatatc
     4981 tctccagagc tcttacacgg gcgacgacag ttacattctt gaattttttc ttccttcgag
     5041 tataacagac gaccaagagc aagatttgct gttgggttct attttggtga caatgaaaga
     5101 acattttcag agtctgaggg ttgcatctgg ggttgacttt ggtgaagatg acgacaaatt
     5161 gtctttcgag atcatccaag cattaccgga caagaaggtt cattcaaaaa tagaatccat
     5221 tcgagttccc ttttctggtt ttaagtcaaa tgcaacagag acgatgttga ttcctcagcc
     5281 tgtggttcag tcttctgatc cagtaaatga gaaaatcaac gtggccactg ttaacggtgt
     5341 ggttaaggag aagaagaaaa cagagaaaaa gcgtgggaag actgagaaaa caatcagtct
     5401 agatgtactt cagcagtatt tcactggaag tctcaaagac gctgcaaaga gcctaggagt
     5461 ttgcccgacg acaatgaagc gaatttgcag gcaacacgga atctcgcggt ggccatcgag
     5521 gaagatcaag aaagtgaatc gttcaatcac aaagctgaaa cgagtcatcg aatctgttca
     5581 aggtactgat ggaggcctcg acctgacttc catggccgtt agttccatcc cttggacaca
     5641 cggtcaaaca tcagcacagc cactaaactc acccaatggt tccaaaccac ctgagctacc
     5701 aaacaccaat aattcaccta accattggtc aagtgatcac agtccgaacg agccaaatgg
     5761 ttcgcctgag ttaccaccaa gcaatggtca caagcgatca cgaacggtgg atgagagcgc
     5821 tgggactcca acctctcatg gctcatgtga cggtaaccaa ttagatgaac cgaaagtccc
     5881 aaatcaagat ccgcttttca cggttggtgg atcacccggg ctcctttttc caccttattc
     5941 tagagatcat gatgtatctg cagcttcctt cgcaatgccg aacaggcttc ttggttctat
     6001 agaccatttc cgaggaatgc tcattgaaga cgctggaagt tcaaaagatc tgagaaatct
     6061 ctgccccact gcagcatttg acgataagtt tcaagacaca aactggatga acaatgataa
     6121 taatagcaac aacaacttat acgctccccc aaaggaagag gccattgcaa atgttgcatg
     6181 cgaaccatca ggctcagaaa tgagaacggt aacaatcaaa gcaagttaca aagacgacat
     6241 aatacggttc agaatatcct cgggttcagg tataatggaa ttgaaggatg aagtggctaa
     6301 gaggctgaaa gttgatgcag gaacgttcga tatcaagtat cttgacgatg ataacgaatg
     6361 ggttttaata gcttgtgatg ctgatcttca agaatgtctc gagatcccta gatcctcccg
     6421 cacgaaaatc gtaaggctct tagttcatga tgtaacgaca aatctaggga gctcctgcga
     6481 gagcactgga gaattgggtt cgggcgggag cggtggaagc ggcgaaattc agcaagccac
     6541 tgcaggagtc tcacaagaca cttcggaaaa tcctaacaaa acaatagttc ctgcagcatt
     6601 accacagctc acccctacct tggtgtcact gctggaggtg attgaacccg aggtgttgta
     6661 tgcaggatat gatagctctg ttccagattc agcatggaga attatgacca cactcaacat
     6721 gttaggtggg cgtcaagtga ttgcagcagt gaaatgggca aaggcgatac taggcttgag
     6781 aaacttacac ctcgatgacc aaatgaccct gctacagtac tcatggatgt ttctcatggc
     6841 atttgccttg ggttggagat catacagaca atcaagcgga aacctgctct gctttgctcc
     6901 tgatctgatt attaatgagc agagaatgtc tctaccctgc atgtatgacc aatgtaaaca
     6961 catgctgttt gtctcctctg aattacaaag attgcaggta tcctatgaag agtatctctg
     7021 tatgaaaacc ttactgcttc tctcctcagt tcctaaggaa ggtctgaaga gtcaagagtt
     7081 atttgatgag attcgaatga cttatatcaa agagctagga aaagccatcg tcaaaaggga
     7141 agggaactcc agtcagaact ggcaacggtt ttaccaactg acaaagcttc tggactccat
     7201 gcatgaggtg gttgagaatc tccttaccta ctgcttccag acatttttgg ataagaccat
     7261 gagtattgaa ttcccagaga tgttagctga aatcatcact aatcagatac caaaatattc
     7321 aaatggaaat atcaaaaagc ttctgtttca tcaaaaatga gcttctctag ctagagtcga
     7381 tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg
     7441 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     7501 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
     7561 atccagatcg ctatactgaa gagcaattct gacaggatat attggcgggt aaacctaaga
     7621 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     7681 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     7741 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa
     7801 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     7861 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     7921 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     7981 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     8041 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     8101 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     8161 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     8221 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     8281 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     8341 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     8401 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     8461 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     8521 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     8581 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     8641 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     8701 agccgcccgc gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     8761 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     8821 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     8881 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     8941 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     9001 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     9061 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     9121 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     9181 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     9241 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     9301 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     9361 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     9421 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     9481 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     9541 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     9601 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     9661 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     9721 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     9781 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     9841 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     9901 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
     9961 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
    10021 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
    10081 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
    10141 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
    10201 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
    10261 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
    10321 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
    10381 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
    10441 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
    10501 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
    10561 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
    10621 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
    10681 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
    10741 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
    10801 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
    10861 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
    10921 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
    10981 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
    11041 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
    11101 ggactctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
    11161 aacccgtaca t
//
