LOCUS       sequence-386959-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with LR dvp promoter: pLBD16-PhiC31-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P32
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..23
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(47..299)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(339..1133)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(450..469)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1060..1079
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1167..1346)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1578..1600
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1592..1609
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1593..1609
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1619..1638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1619..1637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(1647..2288)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(2297..2989)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2572..2591)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2841..2859
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(3005..3025)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5188..5208
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5224..5226,5227..5229,5230..5943)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5269..5290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5530..5549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5877..5898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6622..6646
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6797..7232
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(7512..7531)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7523..8338
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7523..8338
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7596..7615)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8439..9027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8928..8947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggcaggata tattgtggtg taacgttatc agcttgcatg ccggtcgatc tagtaacata
       61 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
      121 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
      181 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
      241 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
      301 gcttgactct agctagagtc cgaaccccag agtcccgctc agaagaactc gtcaagaagg
      361 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
      421 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
      481 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
      541 accatgatat tcggcaagca ggcatcgccg tgggtcacga cgagatcctc gccgtcgggc
      601 atccgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
      661 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
      721 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
      781 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
      841 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
      901 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca
      961 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     1021 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     1081 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcctcga
     1141 tcgagttgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     1201 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     1261 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     1321 cagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     1381 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatcg ataacattaa
     1441 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1501 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1561 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     1621 atacgactca ctatagggcg gagcgattga tgcatgttgt caatcaattg gcaagtcata
     1681 aaatgcatta aaaaatattt tcatactcaa ctacaaatcc atgagtataa ctataattat
     1741 aaagcaatga ttagaatctg acaaggattc tggaaaatta cataaaggaa agttcataaa
     1801 tgtctaaaac acaagaggac atacttgtat tcagtaacat ttgcagcttt tctaggtctg
     1861 aaaatatatt tgttgcctag tgaataagca taatggtaca actacaagtg ttttactcct
     1921 catattaact tcggtcatta gaggccacga tttgacacat ttttactcaa aacaaaatgt
     1981 ttgcatatct cttatatttt caaattcaac acacaacaaa taagagaaaa aacaaataat
     2041 attaatttga gaatgaacaa aaggaccata tcattcatta actcttctcc atccatttcc
     2101 atttcacagt tcgatagcga aaaccgaata aaaaacacag taaattacaa gcacaacaaa
     2161 tggtacaaga aaaacatgtt tcccaatgcc ataatactcg aactcagtag gattctggtg
     2221 tgtgcgcaat gaaactgatg cattgaactt gacgaacgtt gtcgaaaccg atgatacgaa
     2281 cgaaagctca agcttacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
     2341 agcgttcgta ctgttccacc acggtgtagt cctcgttgtg ggaggtgatg tccaacttgc
     2401 ggtcgacgtt gtaggcgccg ggcatctgca cgggcttctt ggccttgtag gtggtcttga
     2461 agtccgccag gtagcggccg ccgtccttca ggcgcagggc catcttaatg tcgcccttca
     2521 gcacgccgtc ctcggggtac aaccgctcgg tggacgcttc ccagcccatt gtcttcttct
     2581 gcattacggg gccgtcagga gggaagttgg tgccgcggag cttcaccttg tagatcaggg
     2641 tgccgtcctc cagggaggtg tcctgggtca cggtcacggc gccgccgtcc tcgaagttca
     2701 tcacgcgctc ccacttgaag ccctcgggga aggactgctt atagtagtcg gggatgtcgg
     2761 cggggtgctt ggtgaaggcc ctggagccgt acatgaactg aggggacagg atgtcccagg
     2821 agaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
     2881 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcatggag ccctccatgt
     2941 gcaccttgaa ccgcatgaac tccttgatca ctgcctcgcc cttgctcaca ccactaccac
     3001 ttccgacttt cctcttcttc tttggcattg tttactatgc gagtttttat ttcgtttatt
     3061 tcaattaagg taactaaaaa actcctttgg agctcaacaa caaactttcc attcacccat
     3121 ttatggatac acagtctagc tcaacagagc ttttaaccca aattggtaca atagaataca
     3181 actttagatc ataattctca aaagaaagag attccttagc tattctatct gccactccat
     3241 ttccttctcg gcttgtatgc acaagcataa aatcctcaaa cttgctaagt agatacttta
     3301 tgtcttggat aattggattg agacttgaca agcataactt tcatgtaacc aaagacacaa
     3361 gttgctgaga atccacctca aaaatgatct tcctataatt gaatcgggat aatgacagca
     3421 cagcccatct aagagcctcc acttctactt ccagcacgct tcttactttt accacagctc
     3481 ttgcacctaa ccataacacc ttccctgtat gatcgcgaag cacccaccct aagccacatt
     3541 ttaatccttc tgttggccat gccccatcaa agttgcactt aacccaagat tgtggtggag
     3601 cttcccatgt ttctcgtctg tcccgacggt gttgtggttg gtgctttcct tacattctga
     3661 gcctctttcc ttctaatcca ctcatctgca tcttcttgtg tccttactaa tacctcattg
     3721 gttccaaatt ccctcccttt aagcaccagc tcgtttctgt tcttccacag cctcccaagt
     3781 atccaaggga ctaaagcctc cacattcttc agatcaggat attcttgttt aagatgttga
     3841 actctatgga ggtttgtatg aactgatgat ctaggaccgg ataagttccc ttcttcatag
     3901 cgaacttatt caaagaatgt tttgtgtatc attcttgtta cattgttatt aatgaaaaaa
     3961 tattattggt cattggactg aacacgagtg ttaaatatgg accaggcccc aaataagatc
     4021 cattgatata tgaattaaat aacaagaata aatcgagtca ccaaaccact tgcctttttt
     4081 aacgagactt gttcaccaac ttgatacaaa agtcattatc ctatgcaaat caataatcat
     4141 acaaaaatat ccaataacac taaaaaatta aaagaaatgg ataatttcac aatatgttat
     4201 acgataaaga agttactttt ccaagaaatt cactgatttt ataagcccac ttgcattaga
     4261 taaatggcaa aaaaaaacaa aaaggaaaag aaataaagca cgaagaattc tagaaaatac
     4321 gaaatacgct tcaatgcagt gggacccacg gttcaattat tgccaatttt cagctccacc
     4381 gtatatttaa aaaataaaac gataatgcta aaaaaatata aatcgtaacg atcgttaaat
     4441 ctcaacggct ggatcttatg acgaccgtta gaaattgtgg ttgtcgacga gtcagtaata
     4501 aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca
     4561 aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca
     4621 acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt
     4681 gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagagctct
     4741 tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct
     4801 ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt
     4861 tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa
     4921 gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga
     4981 tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga
     5041 tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg
     5101 agtttttctg attaacagta ctgcaaaaaa agcaaaaagc atttaccttg attgagatgt
     5161 taattgtgtt ggcataccta aacaatgcca aagaagaaga ggaaagtcgg aagtggtagt
     5221 ggtatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5281 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5341 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5401 accctcgtga ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     5461 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5521 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5581 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5641 cacaagctgg agtacaacta catcagcgac aacgtctata tcaccgccga caagcagaag
     5701 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     5761 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5821 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     5881 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5941 taataaatct cgtctctgtt atgcttaaga agttcaatgt ttcgtttcat gtaaaacttt
     6001 ggtggtttgt gttttggggc cttgtataat ccctgatgaa taagtgttct actatgtttc
     6061 cgttcctgtt atctctttct ttctaatgac aagtcgaact tcttctttat catcgcttcg
     6121 tttttattat ctgtgcttct tttgtttaat acgcctgcaa agtgactcga ctctgtttag
     6181 tgcagttctg cgaaacttgt aaatagtcca attgttggcc tctagtaata gatgtagcga
     6241 aagtgttgag ctgttgggtt ctaaggatgg cttgaacatg ttaatctttt aggttctgag
     6301 tatgatgaac attcgttgtt gctaagaaat gcctgtaatg tcccacaaat gtagaaaatg
     6361 gttcgtacct ttgtccaagc attgatatgt ctgatgagag gaaactgcaa gatactgagc
     6421 ttggtttaac gaaggagagg cagtttcttc cttccaaagc atttcatttg acaatgcctt
     6481 gatcatctta agtagagttt ctgttgtgga aagtttgaaa ctttgaagaa acgactctca
     6541 agtaaattga tgatcacaag tgaaagtgta tgttcgctgc cacagaatca ggggataacg
     6601 caggaaagaa catgaaggcc ttgacaggat atattggcgg gtaaactaag tcgctgtatg
     6661 tgtttgtttg agatctaatt ccggggatcg gaaatccaga agcccgagag gttgccgcct
     6721 ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac gatctgttgc ggccggccgc
     6781 cgggttgtgg gcaaaggcgc tcgacggtgg gcaaccgctt gcggttgtcc acgggcggag
     6841 ccggtgcgcg tagcgcattg tccacaagcc aagggcgacc aataattgat atatatattc
     6901 ataattgaaa agctaattga acatactact tgctgtaact acttgccgga gcgaggggtg
     6961 tttgcaagct gttgatctga aagggctatt agcgttctca cgtgcctttt tgattagcga
     7021 tttcacgtga ccttattagc gatttcacgt actccgatta gcgatttcac gtaccctgat
     7081 tagcgatttc acgtggatag tttttggagc gggccggaaa gccccgtgaa tcaaggcttt
     7141 gcggggcatt agcggtttca cgtggataac taccctctat ccacaggctt ccggggataa
     7201 aaaagcccgc tcgacggcgg gctgttggat ggggatcgcc tgaatcgccc catcatccag
     7261 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat
     7321 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
     7381 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtggctc aaaatctctg
     7441 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     7501 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcaaggcc
     7561 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     7621 cgggcaatca ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt
     7681 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     7741 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     7801 tgcatggtta ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata
     7861 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     7921 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     7981 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     8041 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     8101 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     8161 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     8221 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     8281 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     8341 agaattggtt aattggttgt aaccatgacc aaaatccctt aacgtgagtt ttcgttccac
     8401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9061 gccttttgct ggccttttgc tcacatgaga tct
//
