LOCUS       sequence-386968-        6761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human wild-type PTPN22 with sfCherry(11) tag
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6761)
  TITLE     Concannon Lab Plasmids
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfCherry11-WT-PTPN22
FEATURES             Location/Qualifiers
     source          1..6761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3262..3281)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3263..3281)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3555..3676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3592..3611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3646..3665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3683..4138)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3820..3841)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4032..4051
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4165..4269
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4271..4628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4271..4462
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4294..4314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4479..4614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4663..5457
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4663..5457
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4717..4736)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5327..5346
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5645..5664)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5689..5736
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6065..6653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6554..6573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat gtacaccatc gtggagcagt acgagagagc cgaggccaga
      721 cacagcaccg gtggcggcat ggaccaaaga gaaattctgc agaagttcct ggatgaggcc
      781 caaagcaaga aaattactaa agaggagttt gccaatgaat ttctgaagct gaaaaggcaa
      841 tctaccaagt acaaggcaga caaaacctat cctacaactg tggctgagaa gcccaagaat
      901 atcaagaaaa acagatataa ggatattttg ccctatgatt atagccgggt agaactatcc
      961 ctgataacct ctgatgagga ttccagctac atcaatgcca acttcattaa gggagtttat
     1021 ggacccaagg cttatattgc cacccagggt cctttatcta caaccctcct ggacttctgg
     1081 aggatgattt gggaatatag tgtccttatc attgttatgg catgcatgga gtatgaaatg
     1141 ggaaagaaaa agtgtgagcg ctactgggct gagccaggag agatgcagct ggaatttggc
     1201 cctttctctg tatcctgtga agctgaaaaa aggaaatctg attatataat caggactcta
     1261 aaagttaagt tcaatagtga aactcgaact atctaccagt ttcattacaa gaattggcca
     1321 gaccatgatg taccttcatc tatagaccct attcttgagc tcatctggga tgtacgttgt
     1381 taccaagagg atgacagtgt tcccatatgc attcactgca gtgctggctg tggaaggact
     1441 ggtgttattt gtgctattga ttatacatgg atgttgctaa aagatgggat aattcctgag
     1501 aacttcagtg ttttcagttt gatccgggaa atgcggacac agaggccttc attagttcaa
     1561 acgcaggaac aatatgaact ggtctacaat gctgtattag aactatttaa gagacagatg
     1621 gatgttatca gagataaaca ttctggaaca gagagtcaag caaagcattg tattcctgag
     1681 aaaaatcaca ctctccaagc agactcttat tctcctaatt taccaaaaag taccacaaaa
     1741 gcagcaaaaa tgatgaacca acaaaggaca aaaatggaaa tcaaagaatc ttcttccttt
     1801 gactttagga cttctgaaat aagtgcaaaa gaagagctag ttttgcaccc tgctaaatca
     1861 agcacttctt ttgactttct ggagctaaat tacagttttg acaaaaatgc tgacacaacc
     1921 atgaaatggc agacaaaggc atttccaata gttggggagc ctcttcagaa gcatcaaagt
     1981 ttggatttgg gctctctttt gtttgaggga tgttctaatt ctaaacctgt aaatgcagca
     2041 ggaagatatt ttaattcaaa ggtgccaata acacggacca aatcaactcc ttttgaattg
     2101 atacagcaga gagaaaccaa ggaggtggac agcaaggaaa acttttctta tttggaatct
     2161 caaccacatg attcttgttt tgtagagatg caggctcaaa aagtaatgca tgtttcttca
     2221 gcagaactga attattcact gccatatgac tctaaacacc aaatacgtaa tgcctctaat
     2281 gtaaagcacc atgactctag tgctcttggt gtatattctt acataccttt agtggaaaat
     2341 ccttattttt catcatggcc tccaagtggt accagttcta agatgtctct tgatttacct
     2401 gagaagcaag atggaactgt ttttccttct tctctgttgc caacatcctc tacatccctc
     2461 ttctcttatt acaattcaca tgattcttta tcactgaatt ctccaaccaa tatttcctca
     2521 ctattgaacc aggagtcagc tgtactagca actgctccaa ggatagatga tgaaatcccc
     2581 cctccacttc ctgtacggac acctgaatca tttattgtgg ttgaggaagc tggagaattc
     2641 tcaccaaatg ttcccaaatc cttatcctca gctgtgaagg taaaaattgg aacatcactg
     2701 gaatggggtg gaacatctga accaaagaaa tttgatgact ctgtgatact tagaccaagc
     2761 aagagtgtaa aactccgaag tcctaaatca gaactacatc aagatcgttc ttctccccca
     2821 cctcctctcc cagaaagaac tctagagtcc ttctttcttg ccgatgaaga ttgtatgcag
     2881 gcccaatcta tagaaacata ttctactagc tatcctgaca ccatggaaaa ttcaacatct
     2941 tcaaaacaga cactgaagac tcctggaaaa agtttcacaa ggagtaagag tttgaaaatt
     3001 ttgcgaaaca tgaaaaagag tatctgtaat tcttgcccac caaacaagcc tgcagaatct
     3061 gttcagtcaa ataactccag ctcatttctg aattttggtt ttgcaaaccg tttttcaaaa
     3121 cccaaaggac caaggaatcc accaccaact tggaatattt aataaaactc cagatttata
     3181 ataatatggg ctgcaagtac acctcgaggg ggggcccggt accttaatta attaaggtac
     3241 caggtaagtg tacccaattc gccctatagt gagtcgtatt acaattcact cgatcgccct
     3301 tcccaacagt tgcgcagcct gaatggcgaa tggagatcca atttttaagt gtataatgtg
     3361 ttaaactact gattctaatt gtttgtgtat tttagattca cagtcccaag gctcatttca
     3421 ggcccctcag tcctcacagt ctgttcatga tcataatcag ccataccaca tttgtagagg
     3481 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3541 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3661 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     3721 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3781 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3841 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3901 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3961 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4021 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4081 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     4141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4261 aggaagaatc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     4321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4381 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4441 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4561 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4621 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     4681 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4741 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4801 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     4861 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4921 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4981 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5041 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5101 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagaacatca ggggctcgcg
     5161 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     5221 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5281 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5341 gatattgctg aagaacttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     5401 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     5461 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     5521 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5581 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     5641 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     5701 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     5761 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     5821 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     5881 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     5941 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6001 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6181 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6721 ctgcgttatc ccctgattct gtggataacc gtattaccgc c
//
