LOCUS       sequence-386970-        9304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUBC-NLS-HA-2xMCP-1xGFP-eRF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Li W, Maekiniemi A, Sato H, Osman C, Singer RH
  TITLE     An improved imaging system that corrects MS2-induced RNA 
            destabilization.
  JOURNAL   Nat Methods. 2022 Dec;19(12):1558-1562. doi: 
            10.1038/s41592-022-01658-1. Epub 2022 Nov 10.
  PUBMED    36357695
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01658-1"; journalName: "Nat Methods"; date: 
            "2022-12"; volume: "19"; issue: "12"; pages: "1558-1562"
COMMENT     Sequence Label: yWL229 MCP-GFP-eRF3
FEATURES             Location/Qualifiers
     source          1..9304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2636..2656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2660..2686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2660..2686
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2687..2710
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      3443..3452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3449..3451,3452..3454,3455..4165)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3449..3451,3452..3454,3455..4165)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3494..3515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3755..3774)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4102..4123
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5694..6282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5747..5767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6165..6176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6357..6590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6709..6727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6795..6899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6900..6968,6969..7760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7118..7137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7931..8519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8420..8439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8673..8690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8930..8951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8966..8971,8972..8989,8990..8996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9004..9020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9009..9031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9028..9044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9028..9044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9094..9113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgcgccacca tgggcccaaa
     2641 aaagaaaaga aaagttggct acccctacga cgtgcccgac tacgccctgg aagttctgtt
     2701 ccaggggccc ctgttattaa ttaacgcttc taactttact cagttcgttc tcgtcgacaa
     2761 tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga tcgctgaatg
     2821 gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc gtcagagctc
     2881 tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct ggcgttcgta
     2941 cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg agcttattgt
     3001 taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcagcaa tcgcagcaaa
     3061 ctccggcatc tacgccatgg ccagcaactt cacccagttc gtgctggtgg acaacggcgg
     3121 caccggcgac gtgaccgtgg cccccagcaa cttcgccaac ggcatcgccg agtggatcag
     3181 cagcaacagc agaagccagg cctacaaggt gacctgcagc gtgagacaga gcagcgccca
     3241 gaacagaaag tacaccatca aggtggaggt gcccaagggc gcctggagaa gctacctgaa
     3301 catggagctg accatcccca tcttcgccac caacagcgac tgcgagctga tcgtgaaggc
     3361 catgcagggc ctgctgaagg acggcaaccc catccccagc gccatcgccg ccaacagcgg
     3421 catctacgcc gacccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3661 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3721 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3781 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3841 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3901 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3961 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4021 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4081 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4141 tctcggcatg gacgagctgt acaagggcac aggtgcatgc atggaacttt cagaacctgt
     4201 tgtagaaaat ggagagacag aaatgtcccc agaagaatca tgggagcaca aagaagaaat
     4261 aagtgaagcg gagccagggg gtggttcctc aggagatgga aggccaccag aggaaagcac
     4321 ccaagaaatg atggaggagg aggaagaaat accgaaacct aagtcagcgg tggcaccacc
     4381 aggtgctccc aagaaggaac atgtaaatgt agtgttcatc ggacatgttg atgcaggcaa
     4441 gtcaaccatt ggaggacaaa taatgtattt gactggaatg gtcgacaaaa ggacacttga
     4501 gaagtatgaa cgagaagcta aagaaaaaaa cagagaaact tggtacttgt cttgggcctt
     4561 agacacgaat caggaagaac gagacaaggg caaaaccgtg gaggtgggtc gtgcctattt
     4621 tgaaacggaa aagaagcatt ttacaattct agatgctcct ggccataaga gttttgtccc
     4681 aaatatgatt ggtggtgcct ctcaagctga cttggctgta ctggtaatct cagccaggaa
     4741 aggagagttt gaaactggat ttgaaaaagg aggacagaca agagaacatg caatgttggc
     4801 aaagacagca ggtgtcaaac acttaattgt gcttattaat aagatggatg atccaacggt
     4861 aaactggagc aacgagagat atgaagaatg taaagagaaa ctagtaccat ttttgaaaaa
     4921 agttggcttc aatcccaaaa aggacattca ttttatgccg tgctcaggac tgacaggagc
     4981 gaatcttaaa gaacaatcag atttctgtcc ttggtacatt ggcttaccat ttattccata
     5041 tctggataat ttgccaaact tcaatagatc agttgatgga ccaatcaggc tgccaattgt
     5101 cgataagtac aaggatatgg ggactgtggt cctgggaaag ctggaatctg gatctatttg
     5161 taaaggccag cagcttgtaa tgatgccaaa taagcacaac gtggaagttc ttggaatact
     5221 ttctgatgat gtagaaacgg actctgtagc tccaggtgaa aacctcaaaa tcagactgaa
     5281 aggaattgaa gaggaagaga ttcttccagg attcatcctt tgtgatctta ataatctttg
     5341 tcattctgga cgcacatttg atgctcagat agtgattata gagcacaagt ccatcatctg
     5401 cccagggtat aatgcggtgc tgcatattca tacctgtatc gaagaagtcg aaataacagc
     5461 cttaatctgc ttggtagaca agaaatcagg agagaaaagt aagactcgac cccgttttgt
     5521 aaaacaagat caagtatgca ttgcgcgttt aaggacagca ggaaccatct gcctggagac
     5581 ctttaaggac ttccctcaga tgggacgttt taccttaaga gatgagggta agaccattgc
     5641 aattggaaaa gttctgaaac tggttccaga gaaagactaa atcgatagat cctaatcaac
     5701 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5761 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5821 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5881 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5941 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6001 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6061 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6121 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6181 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6241 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     6301 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6361 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6601 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6661 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     6721 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6961 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7021 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7081 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7141 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7201 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7261 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7321 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7381 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7441 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7501 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7561 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7621 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7681 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7741 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7801 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7861 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8041 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8581 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8641 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8701 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
     8761 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8821 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
     8881 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8941 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9001 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
     9061 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9121 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     9181 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     9241 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     9301 tagt
//
