LOCUS       sequence-386971-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter : 
            p35S:UbGln:PhiC31-NLS:tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P56
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    255..277
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    285..461
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(693..1718)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1738..2083)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2286..2308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2300..2317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2301..2317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2324..2343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2324..2342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3340..3685
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3776..4003
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             4073..5884
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="DTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGG
                     RFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPI
                     VSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELG
                     GYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREI
                     KTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILR
                     DPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAW
                     LDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQ
                     HEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERAN
                     LVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEA
                     PKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIE
                     KRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             5885..5905
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6460..6484
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6575..7163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6655..6674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8672..9487)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8672..9487)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9395..9414
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9479..9498
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9778..10085,1..128)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc ccttggcttg
       61 tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg
      121 tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta taaatttttt
      181 tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga tttccgatcc
      241 ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg gattttagta
      301 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
      361 acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt
      421 tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga gatagatttg
      481 tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg cgaccggccc
      541 tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc caccaagcgt
      601 aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct gaggcgttct
      661 cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg
      721 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
      781 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
      841 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
      901 tggtcaagac caatgcggag catatacgcc cggagccttg gcgatcctgc aagctccgga
      961 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     1021 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     1081 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     1141 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     1201 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     1261 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     1321 aacccgctcg tctggctaag atcggccgca gcgatagcat ccatagcctc cgcgaccggc
     1381 tgaagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     1441 cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac ttccggaatc
     1501 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     1561 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     1621 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     1681 ttctcgacag acgtcgcggt gagttcaggc tttttcatag gggggatcag cttgggctgt
     1741 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     1801 ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct ttgaagacgt
     1861 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     1921 actgtcggca gaggcatctt gaatgatagc ctttccttta tcgcaatgat ggcatttgta
     1981 ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg ggcaatggaa
     2041 tccgaggagg tttcccgaaa ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     2101 tgagactgta tctttgacat ttttggagta ggggtacgat aacattaacg cttacaattt
     2161 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2221 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2281 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     2341 gggcttggag gaattccaat cccacaaaaa tctgagctta acagcacagt tgctcctctc
     2401 agagcagaat cgggtattca acaccctcat atcaactact acgttgtgta taacggtcca
     2461 catgccggta tatacgatga ctggggttgt acaaaggcgg caacaaacgg cgttcccgga
     2521 gttgcacaca agaaatttgc cactattaca gaggcaagag cagcagctga cgcgtacaca
     2581 acaagtcagc aaacagacag gttgaacttc atccccaaag gagaagctca actcaagccc
     2641 aagagctttg ctaaggccct aacaagccca ccaaagcaaa aagcccactg gctcacgcta
     2701 ggaaccaaaa ggcccagcag tgatccagcc ccaaaagaga tctcctttgc cccggagatt
     2761 acaatggacg atttcctcta tctttacgat ctaggaagga agttcgaagg tgaaggtgac
     2821 gacactatgt tcaccactga taatgagaag gttagcctct tcaatttcag aaagaatgct
     2881 gacccacaga tggttagaga ggcctacgca gcaagtctca tcaagacgat ctacccgagt
     2941 aacaatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     3001 aggactaatt gcatcaagaa cacagagaaa gacatatttc tcaagatcag aagtactatt
     3061 ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc
     3121 tctaaaaagg tagttcctac tgaatctaag gccatgcatg gagtctaaga ttcaaatcga
     3181 ggatctaaca gaactcgccg tcaagactgg cgaacagttc atacagagtc ttttacgact
     3241 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctgg tctactccaa
     3301 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat
     3361 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     3421 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
     3481 tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3541 ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccac
     3601 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     3661 aagttcattt catttggaga ggacacgctc gagtataaga gctcattttt acaacaatta
     3721 ccaacaacaa caaacaacaa acaacattac aattacattt acaattatcg atacaatgca
     3781 aatcttcgtt aaaactctga cgggcaaaac gatcacgtta gaagtagagc catccgatac
     3841 gatagaaaac gtgaaggcta aaattcagga caaagaggga ataccgcctg accaacagag
     3901 gctcattttt gcaggcaagc agcttgaaga tggtcgaaca ctgagtgatt acaacatcca
     3961 aaaggagagc actctgcatc tagtcttacg tctgcgaggg ggccaacatg gtagcggtgc
     4021 gtggttgctg cccgtgagtc tcgtgaagag gaagacgact cttgctggag ccgacacgta
     4081 tgccggcgct tatgatagac aatcaagaga aagagagaac tcttctgccg cctctccagc
     4141 tacacagaga tctgccaacg aggataaagc tgctgattta caaagagagg tagagagaga
     4201 tgggggtagg tttagatttg tgggtcattt ctctgaggct cctggtacat cagcctttgg
     4261 tactgctgaa cggcctgaat ttgaaagaat attaaatgag tgtcgggccg gtcgactaaa
     4321 tatgataata gtttatgatg tatctaggtt cagtagactc aaagttatgg atgcaattcc
     4381 gatcgtgagc gagctacttg cactcggtgt tactatcgta agtacacaag aaggtgtatt
     4441 caggcagggt aatgtgatgg accttataca ccttattatg cgactcgacg ctagccataa
     4501 agagtcatca ctgaagagtg ctaaaatctt ggacaccaag aacctgcaaa gggagcttgg
     4561 aggctatgta ggaggaaaag ctccgtatgg tttcgaactc gtgagtgaga caaaagagat
     4621 taccagaaat ggtaggatgg tgaacgtggt tattaataag ctagctcata gcacaacccc
     4681 tctcacaggg ccttttgagt tcgaacctga cgttattaga tggtggtgga gagagatcaa
     4741 aactcacaaa catcttccat ttaagccagg tagccaggct gctatccacc ctggatctat
     4801 cactgggctt tgtaaaagga tggatgctga cgcagtgcca actcgagggg aaacaatcgg
     4861 taagaagact gcatcttccg cttgggatcc cgctacagtt atgagaatcc ttcgggatcc
     4921 gcgtattgct ggattcgccg cagaagtcat ttataagaaa aaacctgatg gtactcctac
     4981 tacaaaaatc gaaggttata ggatccaaag ggatcctatt actttgcgtc cagttgaatt
     5041 agactgtgga ccgatcatcg aacctgccga gtggtacgag ttgcaggcct ggcttgatgg
     5101 tagagggcgt ggaaagggtt tatctcgggg acaggcaatt ttgtcagcaa tggataagct
     5161 ttattgtgag tgtggggctg ttatgacatc aaaacgtgga gaagagtcaa tcaaggattc
     5221 ttacaggtgc agacgtcgta aagtggtgga cccttctgct ccaggacagc atgagggtac
     5281 atgcaacgtt tctatggctg ctcttgataa atttgtggcc gaacgtattt tcaacaagat
     5341 tagacacgct gaaggggatg aggaaacttt agctcttctt tgggaggctg cccggcgttt
     5401 tggtaagcta acagaggcac cagaaaaaag tggcgaaaga gctaatttgg tagccgaaag
     5461 agcagacgcc ttgaatgcat tggaagaact ttacgaagat agggctgccg gagcatatga
     5521 tggaccagtt ggtcggaagc atttcaggaa gcaacaagct gcactaactt tgagacagca
     5581 aggcgcagag gagcgactcg cagagcttga ggccgccgag gccccaaagc ttccactcga
     5641 tcaatggttt cctgaggatg ccgacgctga tcctacaggt ccaaagagct ggtggggaag
     5701 agctagcgta gacgataaga gagtgtttgt gggtctattt gttgacaaga ttgtagttac
     5761 taagtccact acgggtcgag gacagggcac tcccatcgaa aagagagcca gtattacctg
     5821 ggcaaagcct ccaaccgatg atgatgaaga cgatgcacaa gatggtaccg aggatgtggc
     5881 agcaccaaaa aaaaagagga aggtgtaagc ttagagactc ttatcaagaa tcccatctct
     5941 tgcttgcttt tttttgttgt cttccctttg atagggtttg tttttcttgt ttcagtgact
     6001 ttctatgtta aaagataatg tcagtaaaag gatttggttt tctattattc tgaatcgatt
     6061 acggaagatt cttgcttaat tccaatctat acaagtatcg tgaaataatg accgtttatg
     6121 tgattaggag acgtgtttca ttaataaaat ataagatcaa tacattgtta gtagtgataa
     6181 actatgtaca aattgtattg attgtaaaag aaacacaata ggttcctttt ttctacaata
     6241 tattgtgaca gactctctgt tttaacgaat gaattaaatt tgtcaacgaa aaaattaaag
     6301 aatgatgtga tcactttgca ccttggttca tcttcagagg cttctgcatg ttccatgcat
     6361 tgaggctctc tacggtcaca ggttgtgagc cgttgttaaa gacaaacaaa tgcgctacca
     6421 cagaatcagg ggataacgca ggaaagaaca tgaaggcctt gacaggatat attggcgggt
     6481 aaactaagtc gctgtatgtg tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg
     6541 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6601 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6661 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6721 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6781 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6841 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6901 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6961 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7021 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aagaagagtt ggtagctctt
     7081 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7141 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7201 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7261 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtact gagagatccc
     7321 ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag
     7381 ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta
     7441 agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc
     7501 aaccttgttt ctgagcatga acgcccgcaa gccaacatgt tagttgaagc atcagggcga
     7561 ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg gctatggcgt aggcctagtc
     7621 cgtaggcagg acttttcaag tctcggaagg tttcttcaat ctgcattcgc ttcgaataga
     7681 tattaacaag ttgtttgggt gttcgaattt caacaggtaa gttagttgct agaacccatg
     7741 gctcctttgc cgacgctgag tagattttag gtgacgggtg gtgacaatga gtccgtgtcg
     7801 agcgctgatt ttttcggcct ttagagcgag atttatacaa tagaatttgg catgagattg
     7861 gattgctttt agtcagcctc ttatagccta aagtctttga gtgactagat gacatatcat
     7921 gtaagttgct gataggtttc cagttttccg ctcctaggtc tgcatattgt acttttcctc
     7981 ttactcgact taaccagtac caacccagct tctcaacgga tttataccat ggcactttaa
     8041 agccagcatc actgacaatg agcggtgtgg tgttactcgg tagaatgctc gcaaggtcgg
     8101 ctagaaattg gtcatgagct ttctttgaac attgctctga aagcgggaac gctttctcat
     8161 aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg caataccata agtcgttttt
     8221 gctcacgaat atcagaccag tcaacaagta caatgggcat cgtattgccc gaacagataa
     8281 agctagcatg ccaacggtat acagcgagtc gctctttgtg gaggtgacga ttacctaaca
     8341 atcggtcgat tcgtttgatg ttatgttttg ttctcgcttt ggttggcagg ttacggccaa
     8401 gttcggtaag agtgagagtt ttacagtcaa gtaatgcgtg gcaagccaac gttaagctgt
     8461 tgagtcgttt taagtgtaat tcggggcaga attggtaaag agagtcgtgt aaaatatcga
     8521 gttcgcacat cttgttgtct gattattgat ttttcgcgaa accatttgat catatgacaa
     8581 gatgtgtatc caccttaact taatgatttt taccaaaatc attaggggat tcatcagtct
     8641 aaagtatata tgtgtaacat tggtctagtg attagaaaaa ctcatcgagc atcaaatgaa
     8701 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     8761 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     8821 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     8881 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     8941 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     9001 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     9061 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     9121 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     9181 ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     9241 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     9301 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     9361 atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     9421 aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat
     9481 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     9541 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
     9601 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
     9661 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc
     9721 tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag gcgatcccca
     9781 tccaacagcc cgccgtcgag cgggcttttt tatccccgga agcctgtgga tagagggtag
     9841 ttatccacgt gaaaccgcta atgccccgca aagccttgat tcacggggct ttccggcccg
     9901 ctccaaaaac tatccacgtg aaatcgctaa tcagggtacg tgaaatcgct aatcggagta
     9961 cgtgaaatcg ctaataaggt cacgtgaaat cgctaatcaa aaaggcacgt gagaacgcta
    10021 atagcccttt cagatcaaca gcttgcaaac acccctcgct ccggcaagta gttacagcaa
    10081 gtagt
//
