LOCUS       sequence-386974-        9791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter: 
            p35S-PhiC31-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P23
FEATURES             Location/Qualifiers
     source          1..9791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    255..277
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    285..461
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(693..1718)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1738..2083)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2286..2308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2300..2317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2301..2317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2324..2343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2324..2342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3340..3685
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3776..5590
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             5591..5611
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6166..6190
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6281..6869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6361..6380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8378..9193)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8378..9193)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9101..9120
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9185..9204
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9484..9791,1..128)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc ccttggcttg
       61 tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg
      121 tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta taaatttttt
      181 tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga tttccgatcc
      241 ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg gattttagta
      301 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
      361 acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt
      421 tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga gatagatttg
      481 tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg cgaccggccc
      541 tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc caccaagcgt
      601 aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct gaggcgttct
      661 cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg
      721 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
      781 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
      841 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
      901 tggtcaagac caatgcggag catatacgcc cggagccttg gcgatcctgc aagctccgga
      961 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     1021 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     1081 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     1141 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     1201 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     1261 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     1321 aacccgctcg tctggctaag atcggccgca gcgatagcat ccatagcctc cgcgaccggc
     1381 tgaagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     1441 cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac ttccggaatc
     1501 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     1561 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     1621 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     1681 ttctcgacag acgtcgcggt gagttcaggc tttttcatag gggggatcag cttgggctgt
     1741 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     1801 ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct ttgaagacgt
     1861 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     1921 actgtcggca gaggcatctt gaatgatagc ctttccttta tcgcaatgat ggcatttgta
     1981 ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg ggcaatggaa
     2041 tccgaggagg tttcccgaaa ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     2101 tgagactgta tctttgacat ttttggagta ggggtacgat aacattaacg cttacaattt
     2161 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2221 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2281 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     2341 gggcttggag gaattccaat cccacaaaaa tctgagctta acagcacagt tgctcctctc
     2401 agagcagaat cgggtattca acaccctcat atcaactact acgttgtgta taacggtcca
     2461 catgccggta tatacgatga ctggggttgt acaaaggcgg caacaaacgg cgttcccgga
     2521 gttgcacaca agaaatttgc cactattaca gaggcaagag cagcagctga cgcgtacaca
     2581 acaagtcagc aaacagacag gttgaacttc atccccaaag gagaagctca actcaagccc
     2641 aagagctttg ctaaggccct aacaagccca ccaaagcaaa aagcccactg gctcacgcta
     2701 ggaaccaaaa ggcccagcag tgatccagcc ccaaaagaga tctcctttgc cccggagatt
     2761 acaatggacg atttcctcta tctttacgat ctaggaagga agttcgaagg tgaaggtgac
     2821 gacactatgt tcaccactga taatgagaag gttagcctct tcaatttcag aaagaatgct
     2881 gacccacaga tggttagaga ggcctacgca gcaagtctca tcaagacgat ctacccgagt
     2941 aacaatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     3001 aggactaatt gcatcaagaa cacagagaaa gacatatttc tcaagatcag aagtactatt
     3061 ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc
     3121 tctaaaaagg tagttcctac tgaatctaag gccatgcatg gagtctaaga ttcaaatcga
     3181 ggatctaaca gaactcgccg tcaagactgg cgaacagttc atacagagtc ttttacgact
     3241 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctgg tctactccaa
     3301 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat
     3361 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     3421 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
     3481 tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3541 ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccac
     3601 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     3661 aagttcattt catttggaga ggacacgctc gagtataaga gctcattttt acaacaatta
     3721 ccaacaacaa caaacaacaa acaacattac aattacattt acaattatcg atacaatgga
     3781 cacgtatgcc ggcgcttatg atagacaatc aagagaaaga gagaactctt ctgccgcctc
     3841 tccagctaca cagagatctg ccaacgagga taaagctgct gatttacaaa gagaggtaga
     3901 gagagatggg ggtaggttta gatttgtggg tcatttctct gaggctcctg gtacatcagc
     3961 ctttggtact gctgaacggc ctgaatttga aagaatatta aatgagtgtc gggccggtcg
     4021 actaaatatg ataatagttt atgatgtatc taggttcagt agactcaaag ttatggatgc
     4081 aattccgatc gtgagcgagc tacttgcact cggtgttact atcgtaagta cacaagaagg
     4141 tgtattcagg cagggtaatg tgatggacct tatacacctt attatgcgac tcgacgctag
     4201 ccataaagag tcatcactga agagtgctaa aatcttggac accaagaacc tgcaaaggga
     4261 gcttggaggc tatgtaggag gaaaagctcc gtatggtttc gaactcgtga gtgagacaaa
     4321 agagattacc agaaatggta ggatggtgaa cgtggttatt aataagctag ctcatagcac
     4381 aacccctctc acagggcctt ttgagttcga acctgacgtt attagatggt ggtggagaga
     4441 gatcaaaact cacaaacatc ttccatttaa gccaggtagc caggctgcta tccaccctgg
     4501 atctatcact gggctttgta aaaggatgga tgctgacgca gtgccaactc gaggggaaac
     4561 aatcggtaag aagactgcat cttccgcttg ggatcccgct acagttatga gaatccttcg
     4621 ggatccgcgt attgctggat tcgccgcaga agtcatttat aagaaaaaac ctgatggtac
     4681 tcctactaca aaaatcgaag gttataggat ccaaagggat cctattactt tgcgtccagt
     4741 tgaattagac tgtggaccga tcatcgaacc tgccgagtgg tacgagttgc aggcctggct
     4801 tgatggtaga gggcgtggaa agggtttatc tcggggacag gcaattttgt cagcaatgga
     4861 taagctttat tgtgagtgtg gggctgttat gacatcaaaa cgtggagaag agtcaatcaa
     4921 ggattcttac aggtgcagac gtcgtaaagt ggtggaccct tctgctccag gacagcatga
     4981 gggtacatgc aacgtttcta tggctgctct tgataaattt gtggccgaac gtattttcaa
     5041 caagattaga cacgctgaag gggatgagga aactttagct cttctttggg aggctgcccg
     5101 gcgttttggt aagctaacag aggcaccaga aaaaagtggc gaaagagcta atttggtagc
     5161 cgaaagagca gacgccttga atgcattgga agaactttac gaagataggg ctgccggagc
     5221 atatgatgga ccagttggtc ggaagcattt caggaagcaa caagctgcac taactttgag
     5281 acagcaaggc gcagaggagc gactcgcaga gcttgaggcc gccgaggccc caaagcttcc
     5341 actcgatcaa tggtttcctg aggatgccga cgctgatcct acaggtccaa agagctggtg
     5401 gggaagagct agcgtagacg ataagagagt gtttgtgggt ctatttgttg acaagattgt
     5461 agttactaag tccactacgg gtcgaggaca gggcactccc atcgaaaaga gagccagtat
     5521 tacctgggca aagcctccaa ccgatgatga tgaagacgat gcacaagatg gtaccgagga
     5581 tgtggcagca ccaaaaaaaa agaggaaggt gtaagcttag agactcttat caagaatccc
     5641 atctcttgct tgcttttttt tgttgtcttc cctttgatag ggtttgtttt tcttgtttca
     5701 gtgactttct atgttaaaag ataatgtcag taaaaggatt tggttttcta ttattctgaa
     5761 tcgattacgg aagattcttg cttaattcca atctatacaa gtatcgtgaa ataatgaccg
     5821 tttatgtgat taggagacgt gtttcattaa taaaatataa gatcaataca ttgttagtag
     5881 tgataaacta tgtacaaatt gtattgattg taaaagaaac acaataggtt ccttttttct
     5941 acaatatatt gtgacagact ctctgtttta acgaatgaat taaatttgtc aacgaaaaaa
     6001 ttaaagaatg atgtgatcac tttgcacctt ggttcatctt cagaggcttc tgcatgttcc
     6061 atgcattgag gctctctacg gtcacaggtt gtgagccgtt gttaaagaca aacaaatgcg
     6121 ctaccacaga atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg
     6181 gcgggtaaac taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc
     6241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaga agagttggta
     6781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtactgaga
     7021 gatcccctca taatttcccc aaagcgtaac catgtgtgaa taaattttga gctagtaggg
     7081 ttgcagccac gagtaagtct tcccttgtta ttgtgtagcc agaatgccgc aaaacttcca
     7141 tgcctaagcg aactgttgag agtacgtttc gatttctgac tgtgttagcc tggaagtgct
     7201 tgtcccaacc ttgtttctga gcatgaacgc ccgcaagcca acatgttagt tgaagcatca
     7261 gggcgattag cagcatgata tcaaaacgct ctgagctgct cgttcggcta tggcgtaggc
     7321 ctagtccgta ggcaggactt ttcaagtctc ggaaggtttc ttcaatctgc attcgcttcg
     7381 aatagatatt aacaagttgt ttgggtgttc gaatttcaac aggtaagtta gttgctagaa
     7441 cccatggctc ctttgccgac gctgagtaga ttttaggtga cgggtggtga caatgagtcc
     7501 gtgtcgagcg ctgatttttt cggcctttag agcgagattt atacaataga atttggcatg
     7561 agattggatt gcttttagtc agcctcttat agcctaaagt ctttgagtga ctagatgaca
     7621 tatcatgtaa gttgctgata ggtttccagt tttccgctcc taggtctgca tattgtactt
     7681 ttcctcttac tcgacttaac cagtaccaac ccagcttctc aacggattta taccatggca
     7741 ctttaaagcc agcatcactg acaatgagcg gtgtggtgtt actcggtaga atgctcgcaa
     7801 ggtcggctag aaattggtca tgagctttct ttgaacattg ctctgaaagc gggaacgctt
     7861 tctcataaag agtaacagaa cgaccgtgta gtgcgactga agctcgcaat accataagtc
     7921 gtttttgctc acgaatatca gaccagtcaa caagtacaat gggcatcgta ttgcccgaac
     7981 agataaagct agcatgccaa cggtatacag cgagtcgctc tttgtggagg tgacgattac
     8041 ctaacaatcg gtcgattcgt ttgatgttat gttttgttct cgctttggtt ggcaggttac
     8101 ggccaagttc ggtaagagtg agagttttac agtcaagtaa tgcgtggcaa gccaacgtta
     8161 agctgttgag tcgttttaag tgtaattcgg ggcagaattg gtaaagagag tcgtgtaaaa
     8221 tatcgagttc gcacatcttg ttgtctgatt attgattttt cgcgaaacca tttgatcata
     8281 tgacaagatg tgtatccacc ttaacttaat gatttttacc aaaatcatta ggggattcat
     8341 cagtctaaag tatatatgtg taacattggt ctagtgatta gaaaaactca tcgagcatca
     8401 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     8461 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     8521 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     8581 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     8641 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     8701 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     8761 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     8821 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     8881 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     8941 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     9001 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     9061 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     9121 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt
     9181 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     9241 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     9301 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     9361 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     9421 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga
     9481 tccccatcca acagcccgcc gtcgagcggg cttttttatc cccggaagcc tgtggataga
     9541 gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc cttgattcac ggggctttcc
     9601 ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc
     9661 ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga
     9721 acgctaatag ccctttcaga tcaacagctt gcaaacaccc ctcgctccgg caagtagtta
     9781 cagcaagtag t
//
