LOCUS       sequence-386975-        9910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter: 
            p35S-PhiC31-NLS-DST-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P24
FEATURES             Location/Qualifiers
     source          1..9910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    258..280
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    288..464
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(696..1721)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1741..2086)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2289..2311
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2303..2320
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2304..2320
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2327..2346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2327..2345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3343..3688
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3779..5596
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     misc_feature    6288..6312
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6403..6991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6483..6502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8500..9315)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8500..9315)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9223..9242
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9307..9326
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9606..9910,1..131)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 agtatgttca attagctttt caattatgaa tatatatatc aattattggt cgcccttggc
       61 ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca
      121 ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt ttataaattt
      181 ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct ggatttccga
      241 tccccggaat tagatcttgg caggatatat tgtggtgtaa cgttatcgat ctggatttta
      301 gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca
      361 aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat aagaacccta
      421 atttccctta tcgggaaact actcacacat tatttatgga gaaaatagag agagatagat
      481 ttgtagagag agactggtga tttcagcgta ccgaattaat tcctgaatca ctgcgaccgg
      541 ccctcccgcg acccagccga gcgagcttag cgaactgtgg acgagaactg tgccaccaag
      601 cgtaaggccg ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt
      661 tctcgaaatc agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg
      721 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
      781 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
      841 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
      901 agttggtcaa gaccaatgcg gagcatatac gcccggagcc ttggcgatcc tgcaagctcc
      961 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     1021 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     1081 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     1141 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     1201 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     1261 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     1321 ccgaacccgc tcgtctggct aagatcggcc gcagcgatag catccatagc ctccgcgacc
     1381 ggctgaagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
     1441 cggcgggaga tgcaataggt caggctctcg ctgaactccc caatgtcaag cacttccgga
     1501 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     1561 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     1621 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     1681 aacttctcga cagacgtcgc ggtgagttca ggctttttca taggggggat cagcttgggc
     1741 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     1801 ggattgtgcg tcatccctta cgtcagtgga gatgtcacat caatccactt gctttgaaga
     1861 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     1921 accactgtcg gcagaggcat cttgaatgat agcctttcct ttatcgcaat gatggcattt
     1981 gtaggagcca ccttcctttt ctactgtcct ttcgatgaag tgacagatag ctgggcaatg
     2041 gaatccgagg aggtttcccg aaattaccct ttgttgaaaa gtctcaatag ccctttggtc
     2101 ttctgagact gtatctttga catttttgga gtaggggtac gataacatta acgcttacaa
     2161 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2221 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2281 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     2341 atagggcttg gaggaattcc aatcccacaa aaatctgagc ttaacagcac agttgctcct
     2401 ctcagagcag aatcgggtat tcaacaccct catatcaact actacgttgt gtataacggt
     2461 ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa cggcgttccc
     2521 ggagttgcac acaagaaatt tgccactatt acagaggcaa gagcagcagc tgacgcgtac
     2581 acaacaagtc agcaaacaga caggttgaac ttcatcccca aaggagaagc tcaactcaag
     2641 cccaagagct ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca ctggctcacg
     2701 ctaggaacca aaaggcccag cagtgatcca gccccaaaag agatctcctt tgccccggag
     2761 attacaatgg acgatttcct ctatctttac gatctaggaa ggaagttcga aggtgaaggt
     2821 gacgacacta tgttcaccac tgataatgag aaggttagcc tcttcaattt cagaaagaat
     2881 gctgacccac agatggttag agaggcctac gcagcaagtc tcatcaagac gatctacccg
     2941 agtaacaatc tccaggagat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
     3001 ttcaggacta attgcatcaa gaacacagag aaagacatat ttctcaagat cagaagtact
     3061 attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga
     3121 gtctctaaaa aggtagttcc tactgaatct aaggccatgc atggagtcta agattcaaat
     3181 cgaggatcta acagaactcg ccgtcaagac tggcgaacag ttcatacaga gtcttttacg
     3241 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tggtctactc
     3301 caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag
     3361 gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
     3421 gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat
     3481 cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     3541 cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat gtgacatctc
     3601 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     3661 aggaagttca tttcatttgg agaggacacg ctcgagtata agagctcatt tttacaacaa
     3721 ttaccaacaa caacaaacaa caaacaacat tacaattaca tttacaatta tcgatacaat
     3781 ggacacgtat gccggcgctt atgatagaca atcaagagaa agagagaact cttctgccgc
     3841 ctctccagct acacagagat ctgccaacga ggataaagct gctgatttac aaagagaggt
     3901 agagagagat gggggtaggt ttagatttgt gggtcatttc tctgaggctc ctggtacatc
     3961 agcctttggt actgctgaac ggcctgaatt tgaaagaata ttaaatgagt gtcgggccgg
     4021 tcgactaaat atgataatag tttatgatgt atctaggttc agtagactca aagttatgga
     4081 tgcaattccg atcgtgagcg agctacttgc actcggtgtt actatcgtaa gtacacaaga
     4141 aggtgtattc aggcagggta atgtgatgga ccttatacac cttattatgc gactcgacgc
     4201 tagccataaa gagtcatcac tgaagagtgc taaaatcttg gacaccaaga acctgcaaag
     4261 ggagcttgga ggctatgtag gaggaaaagc tccgtatggt ttcgaactcg tgagtgagac
     4321 aaaagagatt accagaaatg gtaggatggt gaacgtggtt attaataagc tagctcatag
     4381 cacaacccct ctcacagggc cttttgagtt cgaacctgac gttattagat ggtggtggag
     4441 agagatcaaa actcacaaac atcttccatt taagccaggt agccaggctg ctatccaccc
     4501 tggatctatc actgggcttt gtaaaaggat ggatgctgac gcagtgccaa ctcgagggga
     4561 aacaatcggt aagaagactg catcttccgc ttgggatccc gctacagtta tgagaatcct
     4621 tcgggatccg cgtattgctg gattcgccgc agaagtcatt tataagaaaa aacctgatgg
     4681 tactcctact acaaaaatcg aaggttatag gatccaaagg gatcctatta ctttgcgtcc
     4741 agttgaatta gactgtggac cgatcatcga acctgccgag tggtacgagt tgcaggcctg
     4801 gcttgatggt agagggcgtg gaaagggttt atctcgggga caggcaattt tgtcagcaat
     4861 ggataagctt tattgtgagt gtggggctgt tatgacatca aaacgtggag aagagtcaat
     4921 caaggattct tacaggtgca gacgtcgtaa agtggtggac ccttctgctc caggacagca
     4981 tgagggtaca tgcaacgttt ctatggctgc tcttgataaa tttgtggccg aacgtatttt
     5041 caacaagatt agacacgctg aaggggatga ggaaacttta gctcttcttt gggaggctgc
     5101 ccggcgtttt ggtaagctaa cagaggcacc agaaaaaagt ggcgaaagag ctaatttggt
     5161 agccgaaaga gcagacgcct tgaatgcatt ggaagaactt tacgaagata gggctgccgg
     5221 agcatatgat ggaccagttg gtcggaagca tttcaggaag caacaagctg cactaacttt
     5281 gagacagcaa ggcgcagagg agcgactcgc agagcttgag gccgccgagg ccccaaagct
     5341 tccactcgat caatggtttc ctgaggatgc cgacgctgat cctacaggtc caaagagctg
     5401 gtggggaaga gctagcgtag acgataagag agtgtttgtg ggtctatttg ttgacaagat
     5461 tgtagttact aagtccacta cgggtcgagg acagggcact cccatcgaaa agagagccag
     5521 tattacctgg gcaaagcctc caaccgatga tgatgaagac gatgcacaag atggtaccga
     5581 ggatgtggca gcataataag cttagtacta tactacaaca tttccatatt ttttttagat
     5641 tgttagctaa tttcccctgg agataattgt aaattgtttc aatgagagga atatacaata
     5701 catagatcgt aattgatcaa tgcgtatttg catgttggta agagactctt atcaagaatc
     5761 ccatctcttg cttgcttttt tttgttgtct tccctttgat agggtttgtt tttcttgttt
     5821 cagtgacttt ctatgttaaa agataatgtc agtaaaagga tttggttttc tattattctg
     5881 aatcgattac ggaagattct tgcttaattc caatctatac aagtatcgtg aaataatgac
     5941 cgtttatgtg attaggagac gtgtttcatt aataaaatat aagatcaata cattgttagt
     6001 agtgataaac tatgtacaaa ttgtattgat tgtaaaagaa acacaatagg ttcctttttt
     6061 ctacaatata ttgtgacaga ctctctgttt taacgaatga attaaatttg tcaacgaaaa
     6121 aattaaagaa tgatgtgatc actttgcacc ttggttcatc ttcagaggct tctgcatgtt
     6181 ccatgcattg aggctctcta cggtcacagg ttgtgagccg ttgttaaaga caaacaaatg
     6241 cgctaccaca gaatcagggg ataacgcagg aaagaacatg aaggccttga caggatatat
     6301 tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca
     6361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6841 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa gaagagttgg
     6901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtactga
     7141 gagatcccct cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag
     7201 ggttgcagcc acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc
     7261 catgcctaag cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg
     7321 cttgtcccaa ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat
     7381 cagggcgatt agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag
     7441 gcctagtccg taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt
     7501 cgaatagata ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag
     7561 aacccatggc tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt
     7621 ccgtgtcgag cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca
     7681 tgagattgga ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga
     7741 catatcatgt aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac
     7801 ttttcctctt actcgactta accagtacca acccagcttc tcaacggatt tataccatgg
     7861 cactttaaag ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc
     7921 aaggtcggct agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc
     7981 tttctcataa agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag
     8041 tcgtttttgc tcacgaatat cagaccagtc aacaagtaca atgggcatcg tattgcccga
     8101 acagataaag ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt
     8161 acctaacaat cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt
     8221 acggccaagt tcggtaagag tgagagtttt acagtcaagt aatgcgtggc aagccaacgt
     8281 taagctgttg agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa
     8341 aatatcgagt tcgcacatct tgttgtctga ttattgattt ttcgcgaaac catttgatca
     8401 tatgacaaga tgtgtatcca ccttaactta atgattttta ccaaaatcat taggggattc
     8461 atcagtctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
     8521 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     8581 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     8641 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     8701 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     8761 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     8821 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     8881 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     8941 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     9001 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     9061 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     9121 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     9181 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     9241 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     9301 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     9361 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     9421 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     9481 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     9541 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     9601 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     9661 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     9721 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     9781 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     9841 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     9901 tacagcaagt
//
