LOCUS       sequence-386979-       10817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 gene expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 10817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ1KN0142
FEATURES             Location/Qualifiers
     source          1..10817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(376..398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    484..624
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     539..558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(639..656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(810..1398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(890..909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1486..2301)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1486..2301)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2209..2228
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2734..2758
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3189..3534
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3477..3497
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    7046..7220
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7241..7265
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8566..9195
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9624..10697
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      join(10763..10817,1..140)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
       61 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
      121 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagtgct gcaaaaagcg
      181 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
      241 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
      301 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
      361 tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag cttgtctgta
      421 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      481 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
      541 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      601 gtaaggagaa aataccgcat caggcgctca tccgcttcct cgctcactga ctcgctgcgc
      661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1441 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattaga aaaactcatc
     1501 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     1561 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     1621 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     1681 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     1741 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     1801 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg
     1861 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     1921 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     1981 gaatgctgtt ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     2041 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     2101 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     2161 gggcttccca tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca
     2221 tttataccca tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt
     2281 ttcccgttga atatggctca taacagaact tattatttcc ttcctctttt ctacagtatt
     2341 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     2401 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     2461 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
     2521 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     2581 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     2641 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     2701 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacat
     2761 aacgaattgc tcttcagcaa ggaggtcaac atggtggagc acgacactct ggtctactcc
     2821 aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt tcaacaaagg
     2881 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     2941 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     3001 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     3061 gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg tgataacatg
     3121 gtggagcacg acactctggt ctactccaaa aatgtcaaag atacagtctc agaagatcaa
     3181 agggctattg agacttttca acaaaggata atttcgggaa acctcctcgg attccattgc
     3241 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc
     3301 catcattgcg ataaaggaaa ggctatcatt caagatctct ctgccgacag tggtcccaaa
     3361 gatggacccc cacccacgag gagcatcgtg gaaaaagaag aggttccaac cacgtctaca
     3421 aagcaagtgg attgatgtga catctccact gacgtaaggg atgacgcaca atcccactat
     3481 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacacgctcg
     3541 agtataagag ctcattttta caacaattac caacaacaac aaacaacaaa caacattaca
     3601 attacattta caattatcga tacaatggaa cttgacgact tggatctcag cggttcttgg
     3661 ccgttagatc agatcacttt tgcttcaaat ttcaagtctc ctgtcatttt ctcttcctcc
     3721 gaacaacctt tctctcctct ctggtccttc tccgaaactt ctggagatgt cggcggcgag
     3781 ctttactccg ccgcagtagc tcccactcgc ttcaccgatt attccgtctt actcgctagt
     3841 tctgaatctg aaaccacgac caaggagaat aatcaggttc cttctccgtc atggggtata
     3901 atgcctttgg agaatccaga tagttactgt gccatcaaag ccaagatgac tcaagcgctt
     3961 agatacttca aggaatctac tggacaacaa catgttcttg ctcaggtttg ggcgcctgtc
     4021 aaaaaccgtg gtcgatatgt tcttacaact tcaggacagc catttgttct tggtcccaat
     4081 agtaatggcc ttaatcaata caggatggtt tcattgacgt atatgttttc ccttgatggt
     4141 gaacgggacg gagagctcgg gcttcctggt cgcgttttca ggaaaaagtt acctgagtgg
     4201 actcctaatg tgcagtatta ctccagcaaa gagttttcaa ggcttggtca cgccttgcac
     4261 tataatgttc agggaacttt ggctttgcct gtttttgaac catccaggca actctgtgta
     4321 ggagttgtcg agcttattat gacttctccg aagattaact atgctcctga ggttgaaaaa
     4381 gtttgtaaag cgcttgaggc cgtgaatctg aaaacttcgg aaatattgaa ccacgagact
     4441 actcagatct gcaacgaggg tcgccaaaat gcgttagctg agattttgga gatactgaca
     4501 gtggtgtgcg aaacctacaa actgccttta gcccagacat gggtcccttg caggcatcgc
     4561 agtgttctag cctttggagg tggttttaag aaaagctgtt cgagcttcga tggaagctgt
     4621 atggggaaag tctgtatgtc tactagtgat ttagcggttt atgttgttga tgctcatgtt
     4681 tggggtttcc gagacgcctg tgctgaacac catctgcaga agggacaagg tgttgctggg
     4741 agggcgtttc agtctggaaa tctatgcttc tgtagagatg ttactcggtt ctgcaaaacc
     4801 gactacccat tagtacacta tgcacggatg ttcaagttga cgagctgttt cgcggtctgc
     4861 ttaaagagca catatacagg ggatgatgag tacgttcttg aattctttct tcctccagcc
     4921 atcacagaca agagtgagca agattgtcta ttgggttctc tgcttcagac aatgaagcag
     4981 cactattcga gtctcaaggt tgtctctgaa actgaactct gtgaaaacaa catgtctctt
     5041 gaagtcgtgg aagcttcgga ggacggaatg gtttactcga aacttgaacc aatcaggata
     5101 catcatccag cacaaatctc taaggattac cttgagctca acgcacccga acagaaggtg
     5161 agcttaaact ctgactttat ggagaataat gaagttgatg atggggttga gaggttccaa
     5221 acgcttgatc ctataccaga ggccaaaaca gtgaaaaaat cagaaaggaa gcgtgggaaa
     5281 acagagaaaa cgatcagtct agaagtgctt caacaatatt ttgctggtag tctaaaagat
     5341 gcagcaaaga gcctcggagt ttgcccaaca acaatgaaac gcatttgccg acaacacggg
     5401 atctcacggt ggccatctcg aaaaatcaac aaggtgaacc gatctctcac acgacttaaa
     5461 catgttatag actcggttca aggtgctgat ggatcactca acttgacgtc cctctctcct
     5521 cgcccttggc ctcaccaaat ccctccaatc gatatccaat tagcaaagaa ctgccctcca
     5581 acttctacat ctcccctaag taatctccaa gatgtcaaaa tcgaaaacag agatgcagaa
     5641 gacagtgcag gttcatcaac atcgcgtgct tcttgcaaag tgaatcctat ttgcgagacg
     5701 cgttttcggc ttcctacaca caaccaagaa ccatccagac aagtggcctt ggacgattcg
     5761 gatagtagct ctaaaaacat gacaaatttt tgggcacact taacctgcca agatacagct
     5821 tctccaacaa ttctccaaca taagctcgtg agtataaagg caacgtacag agaagacatc
     5881 atcaggttca agatctcacc agagtcggta agcattacgg aacttaagca gcaagtggcc
     5941 aaaaggctga agctcgaaac ggcagccttt gagctcaagt atctagacga cgatagagaa
     6001 tgggtgtccg tctcatgcga tgctgacctc agcgaatgcc tcgacacgtc tgcagcaaaa
     6061 gcgaacacac tgaggctctc agtacatgac gtgactttta actttgggag ctcatgcgaa
     6121 agctcagagg aaactatgat gtgcttgggt tcgggcggga gcggtggaag cggcgaaatt
     6181 cagcaagcca ctgcaggagt ctcacaagac acttcggaaa atcctaacaa aacaatagtt
     6241 cctgcagcat taccacagct cacccctacc ttggtgtcac tgctggaggt gattgaaccc
     6301 gaggtgttgt atgcaggata tgatagctct gttccagatt cagcatggag aattatgacc
     6361 acactcaaca tgttaggtgg gcgtcaagtg attgcagcag tgaaatgggc aaaggcgata
     6421 ctaggcttga gaaacttaca cctcgatgac caaatgaccc tgctacagta ctcatggatg
     6481 tttctcatgg catttgcctt gggttggaga tcatacagac aatcaagcgg aaacctgctc
     6541 tgctttgctc ctgatctgat tattaatgag cagagaatgt ctctaccctg catgtatgac
     6601 caatgtaaac acatgctgtt tgtctcctct gaattacaaa gattgcaggt atcctatgaa
     6661 gagtatctct gtatgaaaac cttactgctt ctctcctcag ttcctaagga aggtctgaag
     6721 agtcaagagt tatttgatga gattcgaatg acttatatca aagagctagg aaaagccatc
     6781 gtcaaaaggg aagggaactc cagtcagaac tggcaacggt tttaccaact gacaaagctt
     6841 ctggactcca tgcatgaggt ggttgagaat ctccttacct actgcttcca gacatttttg
     6901 gataagacca tgagtattga attcccagag atgttagctg aaatcatcac taatcagata
     6961 ccaaaatatt caaatggaaa tatcaaaaag cttctgtttc atcaaaaatg agcttctcta
     7021 gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     7081 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     7141 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     7201 ccagtactaa aatccagatc gctatactga agagcaattc tgacaggata tattggcggg
     7261 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     7321 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     7381 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     7441 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     7501 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     7561 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     7621 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     7681 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     7741 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     7801 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     7861 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     7921 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     7981 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     8041 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     8101 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     8161 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     8221 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     8281 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     8341 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg
     8401 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     8461 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     8521 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     8581 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     8641 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     8701 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     8761 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     8821 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     8881 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     8941 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     9001 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     9061 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     9121 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     9181 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     9241 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     9301 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     9361 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     9421 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     9481 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     9541 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga
     9601 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     9661 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     9721 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     9781 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     9841 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     9901 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     9961 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
    10021 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
    10081 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
    10141 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
    10201 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
    10261 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
    10321 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
    10381 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
    10441 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
    10501 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
    10561 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
    10621 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
    10681 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
    10741 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
    10801 ttgggaaccg gaacccg
//
