LOCUS       sequence-386984-        8004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Epitope tagging of effector protein SseG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8004)
  TITLE     Hensel lab plasmids
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p2888
FEATURES             Location/Qualifiers
     source          1..8004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     348..367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(586..690)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(716..1171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(853..874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1065..1084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1298..1320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1312..1329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1313..1329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1339..1358
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1339..1357
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1382..1398
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1383..1399
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3049..3075
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3877..3998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3914..3933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3968..3987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4056..4076)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4056..4074)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4095..4111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4095..4111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4108..4130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4119..4135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4143..4149,4150..4167,4168..4173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4188..4209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4326..4343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5488..5710
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5758..6708
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(7729..8004,1..516,517..585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
       61 ctcgtcgttt ggtatggctt cattcagctc cggttcccag cgatcaaggc gagttacatg
      121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
      721 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
      781 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
      901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
      961 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     1021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1141 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     1201 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     1261 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     1321 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc
     1381 ctcgaggtcg acggtatcga taagcttaga agagaacaac ggcaagttac aggatccgca
     1441 gcaatatcag caaaacacct tattgcttga agcgatcgag caggccgaaa atatcatcaa
     1501 cattatttat tatcgttacc ataacagcgc acttgtagtg agtgagcaag agtaaagtaa
     1561 aaatatctta gagcctatcc caccaggcgt taattggcgc agccagtttg gacacggata
     1621 gcgcgcaaaa accgcagcgt acacgtagta cgtgaggttt gactcgctac gctcgccctt
     1681 cgggccgccg ctagcggcgt tcaaaacgct aacgcgtttt ggcgagcact gcccaggttc
     1741 aaaatggcaa gtaaaatagc cctaatggga taggctctta gttagcacgt taattatctt
     1801 atcgtgaatt caataggtat gatgatgaaa gaagatcaga aaaataaaat acccgaagac
     1861 attctgaaac agctattatc cgttgatccg gaaaccgttt atgccagtgg ttacgcctca
     1921 tggcaggagg gggattattc gcgcgccgta atcgatttta gttggctggt gatggcccag
     1981 ccatggagtt ggcgtgccca tattgcattg gctggcacct ggatgatgct taaagaatac
     2041 acgacggcca ttaatttcta tggacatgcc ttgatgctgg atgccagcca tccagaaccg
     2101 gtttaccaaa cgggcgtctg tctcaaaatg atgggggaac ccgggttggc gagagaggct
     2161 tttcaaaccg caatcaagat gagttatgcg gatgcctcat ggagtgagat tcgccagaat
     2221 gcgcaaataa tggttgatac tcttattgct taaataacag aacgaaatat gaaaattcat
     2281 attccgtcag cggcaagtaa tatagtcgat ggtaatagtc ctccttccga tatacaagcg
     2341 aaggaggtat cgtttcctcc ccctgaaatt ccagcgcctg gcacccccgc agcccctgtg
     2401 ctgcttacgc ctgaacaaat aaggcagcag agggattatg cgatacattt tatgcaatac
     2461 actattcgtg cgctgggtgc gacagtcgtg tttgggttat cggttgctgc agcggtaatt
     2521 tctggcgggg caggattacc cattgctatt cttgcggggg cggcgctcgt gattgctatt
     2581 ggggatgctt gctgtgcgta tcataattat caatcgatat gtcagcaaaa ggagccatta
     2641 caaaccgcca gtgatagcgt tgctcttgtg gtcagtgcgc tggccttaaa atgtggggca
     2701 agtcttaact gcgctaacac ccttgctaat tgtctttctt tattaatacg ttcaggaatc
     2761 gctatttcta tgttggtttt acccctacag tttccactgc ccgcggctga aaatattgcg
     2821 gcctctttgg acatggggag tgtaattacc tccgttagcc tgacggcgat aggtgcggta
     2881 ctggattatt gccttgcccg cccctctggc gacgatcagg aaaattctgt tgatgaactt
     2941 catgccgatc ccagtgtgtt attggcggaa caaatggcag cgctctgtca atctgctact
     3001 acacctgcat taatggacag ttctgatcat acatctcggg gagaaccata cccatacgac
     3061 gtcccagact acgcttaatc tagatctcgg ggagaaccat gaaacctgtt agcccaaatg
     3121 ctcaggtagg agggcaacgt cctgttaacg cgcctgagga atcacctcca tgtccttcat
     3181 tgccacatcc ggaaaccaat atggagagtg gtagaatagg acctcaacaa ggaaaagagc
     3241 gggtattggc cggacttgcg aaacgagtga tagagtgttt tccaaaagaa atttttagtt
     3301 ggcaaacggt tattttgggc ggacagattt tatgctgttc cgctggaata gcattaacag
     3361 tgctaagtgg tggaggcgcg ccgctcgtag ccctggcagg gattggcctt gctattgcca
     3421 tcgcggatgt cgcctgtctt atctaccatc ataaacatca tttgcctatg gctcacgaca
     3481 gtataggcaa tgccgttttt tatattgcta attgtttcgc caatcaacgc aaaagtatgg
     3541 cgattgctaa agccgtctcc ctgggcggta gattagcctt aaccgcgacg gtaatgactc
     3601 attcatactg gagtggtagt ttgggactac agcctcattt attagagcgt cttaatgata
     3661 ttacctatgg actaatgagt tttactcgct tcggtatgga tgggatggca atgaccggta
     3721 tgcaggtcag cagcccatta tatcgtttgc tggctcaggt aacgccagaa caacgtgcgc
     3781 cggatggggg tggtgccatg gatcggagta gggatcgcct acctcctttt gagacagaga
     3841 cgcggatcct ctagttctag gatcttatta aagcagaact tgtttattgc agcttataat
     3901 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3961 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagag
     4021 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     4081 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4141 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4201 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4261 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4441 tgagatctct acgggtcgga tttgaagtcg tcttggtagg aggcagcctg aatggcgaat
     4501 gccgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     4561 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac agggtgccgg
     4621 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     4681 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     4741 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     4801 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     4861 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     4921 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     4981 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     5041 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     5101 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     5161 tccgcagacc cgccataaaa cgccctgaga agcccgtgac gggcttttct tgtattatgg
     5221 gtagtttcct tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc ccattcactg
     5281 ccagagccgt gagcgcagcg aactgaatgt cacgaaaaag acagcgactc aggtgcctga
     5341 tggtcggaga caaaaggaat attcagcgat ttgcccgagc ttgcgagggt gctacttaag
     5401 cctttagggt tttaaggtct gttttgtaga ggagcaaaca gcgtttgcga catccttttg
     5461 taatactgcg gaactgacta aagtagtgag ttatacacag ggctgggatc tattcttttt
     5521 atcttttttt attctttctt tattctataa attataacca cttgaatata aacaaaaaaa
     5581 acacacaaag gtctagcgga atttacagag ggtctagcag aatttacaag ttttccagca
     5641 aaggtctagc agaatttaca gatacccaca actcaaagga aaaggactag taattatcat
     5701 tgactagccc atctcaattg gtatagtgat taaaatcacc tagaccaatt gagatgtatg
     5761 tctgaattag ttgttttcaa agcaaatgaa ctagcgatta gtcgctatga cttaacggag
     5821 catgaaacca agctaatttt atgctgtgtg gcactactca accccacgat tgaaaaccct
     5881 acaaggaaag aacggacggt atcgttcact tataaccaat acgctcagat gatgaacatc
     5941 agtagggaaa atgcttatgg tgtattagct aaagcaacca gagagctgat gacgagaact
     6001 gtggaaatca ggaatccttt ggttaaaggc tttgagattt tccagtggac aaactatgcc
     6061 aagttctcaa gcgaaaaatt agaattagtt tttagtgaag agatattgcc ttatcttttc
     6121 cagttaaaaa aattcataaa atataatctg gaacatgtta agtcttttga aaacaaatac
     6181 tctatgagga tttatgagtg gttattaaaa gaactaacac aaaagaaaac tcacaaggca
     6241 aatatagaga ttagccttga tgaatttaag ttcatgttaa tgcttgaaaa taactaccat
     6301 gagtttaaaa ggcttaacca atgggttttg aaaccaataa gtaaagattt aaacacttac
     6361 agcaatatga aattggtggt tgataagcga ggccgcccga ctgatacgtt gattttccaa
     6421 gttgaactag atagacaaat ggatctcgta accgaacttg agaacaacca gataaaaatg
     6481 aatggtgaca aaataccaac aaccattaca tcagattcct acctacataa cggactaaga
     6541 aaaacactac acgatgcttt aactgcaaaa attcagctca ccagttttga ggcaaaattt
     6601 ttgagtgaca tgcaaagtaa gtatgatctc aatggttcgt tctcatggct cacgcaaaaa
     6661 caacgaacca cactagagaa catactggct aaatacggaa ggatctgagg ttcttatggc
     6721 tcttgtatct atcagtgaag catcaagact aacaaacaaa agtagaacaa ctgttcaccg
     6781 ttacatatca aagggaaaac tgtccatatg cacagatgaa aacggtgtaa aaaagataga
     6841 tacatcagag cttttacgag tttttggtgc attcaaagct gttcaccatg aacagatcga
     6901 caatgtaaca gatgaacagc atgtaacacc taatagaaca ggtgaaacca gtaaaacaaa
     6961 gcaactagaa catgaaattg aacacctgag acaacttgtt acagctcaac agtcacacat
     7021 agacagcctg aaacaggcga tgctgcttat cgaatcaaag ctgccgacaa cacgggagcc
     7081 agtgacgcct cccgtgggga aaaaatcatg gcaattctgg aagaaatagc gctttcagcc
     7141 ggcaaaccgg ctgaagccgg atctgcgatt ctgataacaa actagcaaca ccagaacagc
     7201 ccgtttgcgg gcagcaaaac ccgtactttt ggacgttccg gcggtttttt gtggcgagtg
     7261 gtgttcgggc ggtgcgcgca agatccatta tgttaaacgg gcgagtttac atctcaaaac
     7321 cgcccgctta acaccatcag aaatcctcag cgcgatttta agcaccaacc cccccccgta
     7381 acacccaaat ccatactgaa agtggctttg ttgaataaat cagatttcgg gtaagtctcc
     7441 cccgtagcgg gttgtgtttt caggcaatac gcacgctttc aggcatacct gctttcgtca
     7501 ttttgttcag cgctcgtacc agggccatag cctccgcaac ctgaccatcg tagtcacgca
     7561 gcgtcagtga acccccgaac agagatctga cgctcagtgg aacgaaaact cacgttaagg
     7621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7981 ttgccgggaa gctagagtaa gtag
//
