LOCUS       sequence-387000-        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible BAF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: BAF-S4A-tet-puro
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(318..342)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             360..377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        387..871
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     817..835
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(907..926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gggcggggat ggctagcatc acagtttgta caaaaaagca ggcttcacca tgacaaccgc
       61 ccagaaacat cgtgacttcg tggcagagcc catgggggag aagccagtgg ggagcctggc
      121 tgggattggt gaagtcctgg gcaagaagct ggaggaaagg ggttttgaca aggcctatgt
      181 tgtccttggc cagtttctgg tgctaaagaa agatgaagac ctcttccggg aatggctgaa
      241 agacacttgt ggcgccaacg ccaagcagtc ccgggactgc ttcggatgcc ttcgagagtg
      301 gtgcgacgcc ttcttgcacc cagctttctt gtacaaagtg gtttagtaat gaaccggtcc
      361 accaccacca ccaccactaa ggatccgggg ttggggttgc gccttttcca aggcagccct
      421 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
      481 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
      541 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
      601 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
      661 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
      721 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
      781 ggggcgggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
      841 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctcccagcaa
      901 ttcaccatga ccgagtacaa gcccacggtg cgcctcgcac ccgcgacgac atccccaggg
      961 gcgtacgcac cctcgccgcc gcggtcgccg actaccccgc cacgcgccac accgtcgatc
     1021 cggacagccc atcgagcggg tcacgagctg caggaatctt ctctactcgc gtctggctcg
     1081 acatcgcaag gtggggtgcc gggaaaagtc cccgtggcgt ctgaacacta ccgaaacagc
     1141 caaccgaggc ggggttctcg aatcagaccc gcatggcnan nagcggtcng ctgggccgca
     1201 ncaccaatga agacatcatg agtcgtctcg tgcacg
//
