LOCUS       sequence-387001-        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible BAF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: BAF-S4D-tet-puro
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(315..339)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             357..374
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        384..864
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 gtggatggct agcatcacaa gtttgtacaa aaaagcaggc ttcaccatga caaccgacca
       61 gaaacatcgt gacttcgtgg cagagcccat gggggagaag ccagtgggga gcctggctgg
      121 gattggtgaa gtcctgggca agaagctgga ggaaaggggt tttgacaagg cctatgttgt
      181 ccttggccag tttctggtgc taaagaaaga tgaagacctc ttccgggaat ggctgaaaga
      241 cacttgtggc gccaacgcca agcagtcccg ggactgcttc ggatgccttc gagagtggtg
      301 cgacgccttc ttgcacccag ctttcttgta caaagtggtt tagtaatgaa ccggtccacc
      361 accaccacca ccactaagga tccggggttg gggttgcgcc ttttccaagg cagccctggg
      421 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
      481 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
      541 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
      601 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
      661 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
      721 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
      781 ggcggtagtg tgagccctgt tcctgcgccc gcgtgttccc ccttctgcga gctccagacg
      841 cacgtcgcag tcggctcagt cgttgacgga atcacccacc tctctccgca cagttcacca
      901 tgaccaatac gagcacgcgc tgcgcttcac ccccgcgaca ccgccccagg accctagaac
      961 ccaaacaccg gattgccaac gccccacagg tcccatagag attgggagcc ggaagagctg
     1021 aaagaacgtg aaaaatgtac aacactctcc gtgtctaccc acaaagttta atgtgagaaa
     1081 agcctccggg gagatataac acatcgaaga gaagagtgct gtgagatgga caagaacaac
     1141 acagagttag aggatagcat agtagagcga cacgaaactg aagcagtagt gacccataca
     1201 caaggagctg atagtacggt cg
//
