LOCUS       sequence-387013-        9629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 gene expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPSW1KN0027
FEATURES             Location/Qualifiers
     source          1..9629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      313..507
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(743..765)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    851..991
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     906..925
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1006..1023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1177..1765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1257..1276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1853..2668)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1853..2668)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2576..2595
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    3101..3125
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3229..3574
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3517..3537
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    6225..6399
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6420..6444
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7745..8374
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(8803..9629,1..247)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
       61 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
      121 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
      181 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
      241 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
      301 aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
      361 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
      421 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
      481 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagtgctgca
      541 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
      601 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
      661 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
      721 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg gtcacagctt
      781 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
      841 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
      901 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
      961 cagatgcgta aggagaaaat accgcatcag gcgctcatcc gcttcctcgc tcactgactc
     1021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1801 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt gattagaaaa
     1861 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     1921 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     1981 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     2041 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     2101 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     2161 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     2221 cgagtcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     2281 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     2341 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag
     2401 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     2461 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     2521 gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc
     2581 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc
     2641 aagacgtttc ccgttgaata tggctcataa cagaacttat tatttccttc ctcttttcta
     2701 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
     2761 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
     2821 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg
     2881 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
     2941 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
     3001 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
     3061 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
     3121 taaacataac gaattgctct tcagcaagga ggtcaacatg gtggagcacg acactctggt
     3181 ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca
     3241 acaaaggata atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     3301 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
     3361 ggctatcatt caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     3421 gagcatcgtg gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga
     3481 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     3541 tatataagga agttcatttc atttggagag gacacgctcg agtataagag ctcattttta
     3601 caacaattac caacaacaac aaacaacaaa caacattaca attacattta caattatcga
     3661 tacaatggaa cttgacgact tggatctcag cggttcttgg ccgttagatc agatcacttt
     3721 tgcttcaaat ttcaagtctc ctgtcatttt ctcttcctcc gaacaacctt tctctcctct
     3781 ctggtccttc tccgaaactt ctggagatgt cggcggcgag ctttactccg ccgcagtagc
     3841 tcccactcgc ttcaccgatt attccgtctt actcgctagt tctgaatctg aaaccacgac
     3901 caaggagaat aatcaggttc cttctccgtc atggggtata atgcctttgg agaatccaga
     3961 tagttactgt gccatcaaag ccaagatgac tcaagcgctt agatacttca aggaatctac
     4021 tggacaacaa catgttcttg ctcaggtttg ggcgcctgtc aaaaaccgtg gtcgatatgt
     4081 tcttacaact tcaggacagc catttgttct tggtcccaat agtaatggcc ttaatcaata
     4141 caggatggtt tcattgacgt atatgttttc ccttgatggt gaacgggacg gagagctcgg
     4201 gcttcctggt cgcgttttca ggaaaaagtt acctgagtgg actcctaatg tgcagtatta
     4261 ctccagcaaa gagttttcaa ggcttggtca cgccttgcac tataatgttc agggaacttt
     4321 ggctttgcct gtttttgaac catccaggca actctgtgta ggagttgtcg agcttattat
     4381 gacttctccg aagattaact atgctcctga ggttgaaaaa gtttgtaaag cgcttgaggc
     4441 cgtgaatctg aaaacttcgg aaatattgaa ccacgagact actcagatct gcaacgaggg
     4501 tcgccaaaat gcgttagctg agattttgga gatactgaca gtggtgtgcg aaacctacaa
     4561 actgccttta gcccagacat gggtcccttg caggcatcgc agtgttctag cctttggagg
     4621 tggttttaag aaaagctgtt cgagcttcga tggaagctgt atggggaaag tctgtatgtc
     4681 tactagtgat ttagcggttt atgttgttga tgctcatgtt tggggtttcc gagacgcctg
     4741 tgctgaacac catctgcaga agggacaagg tgttgctggg agggcgtttc agtctggaaa
     4801 tctatgcttc tgtagagatg ttactcggtt ctgcaaaacc gactacccat tagtacacta
     4861 tgcacggatg ttcaagttga cgagctgttt cgcggtctgc ttaaagagca catatacagg
     4921 ggatgatgag tacgttcttg aattctttct tcctccagcc atcacagaca agagtgagca
     4981 agattgtcta ttgggttctc tgcttcagac aatgaagcag cactattcga gtctcaaggt
     5041 tgtctctgaa actgaactct gtgaaaacaa catgtctctt gaagtcgtgg aagcttcgga
     5101 ggacggaatg gtttactcga aacttgaacc aatcaggata catcatccag cacaaatctc
     5161 taaggattac cttgagctca acgcacccga acagaaggtg agcttaaact ctgactttat
     5221 ggagaataat gaagttgatg atggggttga gaggttccaa acgcttgatc ctataccaga
     5281 ggccaaaaca gtgaaaaaat cagaaaggaa gcgtgggaaa acagagaaaa cgatcagtct
     5341 agaagtgctt caacaatatt ttgctggtag tctaaaagat gcagcaaaga gcctcggagt
     5401 ttgcccaaca acaatgaaac gcatttgccg acaacacggg atctcacggt ggccatctcg
     5461 aaaaatcaac aaggtgaacc gatctctcac acgacttaaa catgttatag actcggttca
     5521 aggtgctgat ggatcactca acttgacgtc cctctctcct cgcccttggc ctcaccaaat
     5581 ccctccaatc gatatccaat tagcaaagaa ctgccctcca acttctacat ctcccctaag
     5641 taatctccaa gatgtcaaaa tcgaaaacag agatgcagaa gacagtgcag gttcatcaac
     5701 atcgcgtgct tcttgcaaag tgaatcctat ttgcgagacg cgttttcggc ttcctacaca
     5761 caaccaagaa ccatccagac aagtggcctt ggacgattcg gatagtagct ctaaaaacat
     5821 gacaaatttt tgggcacact taacctgcca agatacagct tctccaacaa ttctccaaca
     5881 taagctcgtg agtataaagg caacgtacag agaagacatc atcaggttca agatctcacc
     5941 agagtcggta agcattacgg aacttaagca gcaagtggcc aaaaggctga agctcgaaac
     6001 ggcagccttt gagctcaagt atctagacga cgatagagaa tgggtgtccg tctcatgcga
     6061 tgctgacctc agcgaatgcc tcgacacgtc tgcagcaaaa gcgaacacac tgaggctctc
     6121 agtacatgac gtgactttta actttgggag ctcatgcgaa agctcagagg aaactatgat
     6181 gtgcttgtga gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
     6241 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     6301 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     6361 taattcctaa aaccaaaatc cagtactaaa atccagatcg ctatactgaa gagcaattct
     6421 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     6481 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     6541 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     6601 ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg
     6661 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     6721 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     6781 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     6841 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     6901 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     6961 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     7021 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     7081 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     7141 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     7201 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     7261 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     7321 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     7381 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     7441 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     7501 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca accgtgcggc
     7561 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     7621 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     7681 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     7741 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     7801 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     7861 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     7921 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     7981 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     8041 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     8101 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     8161 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     8221 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     8281 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     8341 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     8401 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     8461 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     8521 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     8581 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     8641 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     8701 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     8761 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     8821 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     8881 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     8941 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     9001 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     9061 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     9121 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     9181 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     9241 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     9301 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     9361 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     9421 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     9481 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     9541 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     9601 gattactttt tgatcgatcc cggcatcgg
//
