LOCUS       sequence-387016-       11451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-3 delta
            fusion protein in yeast (yeast two-hybrid or yeast three-hybrid 
            assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Mattera R, Boehm M, Chaudhuri R, Prabhu Y, Bonifacino JS
  TITLE     Conservation and diversification of dileucine signal recognition by 
            adaptor protein (AP) complex variants.
  JOURNAL   J Biol Chem. 2011 Jan 21;286(3):2022-30. doi: 
            10.1074/jbc.M110.197178. Epub 2010 Nov 19.
  PUBMED    21097499
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.197178"; journalName: "J Biol Chem"; date: 
            "2011-01-21- 21"; volume: "286"; issue: "3"; pages: "2022-30"
COMMENT     Sequence Label: pGADT7-delta
FEATURES             Location/Qualifiers
     source          1..11451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      5879..6066
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5934..5958)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(6183..7277)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7278..7683)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7725..7741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7725..7741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7749..7765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7773..7779,7780..7797,7798..7803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7818..7839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7894..7927
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8005..8038
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8107..8124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8278..8866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8358..8377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9037..9828,9829..9897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9660..9679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9898..10002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10070..10088
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10126..10148)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10248..10267
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      10284..11448
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attccacccg ggtgggcatc gatacgggat ccgaattccg atggccctca
     2041 agatggtgaa gggcagcatc gaccgcatgt tcgacaagaa tctgcaggac ttggtccgcg
     2101 gcatccgtaa ccacaaggag gacgaggcaa aatacatatc tcagtgcatt gatgagatca
     2161 agcaggagct gaagcaggac aacatagcgg tgaaggcgaa cgcggtctgc aagctgacgt
     2221 atttacagat gttgggatac gacatcagct gggccgcctt caacatcata gaagtgatga
     2281 gtgcctccaa gttcaccttc aagcgaattg gctacctcgc tgcttcccag agctttcacg
     2341 aaggcaccga cgtcatcatg ctgaccacca atcagatccg taaggacttg agcagcccca
     2401 gccagtacga cacaggtgtt gcactgacgg gtctgtcctg cttcgtcacc ccagaccttg
     2461 ccagagacct ggcaaatgac atcatgacac tgatgtcaca caccaagccc tacatcagga
     2521 agaaggctgt gctgatcatg tacaaggtgt tcctgaagta ccccgagtcg ctgcgccctg
     2581 cctttccccg gctgaaggag aagctggagg accccgaccc cggggttcag tcggctgccg
     2641 tcaatgtcat ctgcgagctg gccagacgca accctaagaa ctacctgtcc ctggccccgc
     2701 tctttttcaa gctgatgacg tcctccacca acaactgggt cctcatcaag atcatcaagc
     2761 tgttcggtgc tcttactcct ttggaaccgc ggctgggcaa gaagctgatc gagcccctca
     2821 ccaatctcat ccacagcacg tctgccatgt ctctcctcta tgaatgtgtg aacaccgtga
     2881 ttgcagtgct catctcgctg tcctccggca tgcccaacca cagcgccagc atccagcttt
     2941 gtgttcagaa attaaggata ttgatcgagg actccgatca gaacttgaag tacctggggc
     3001 tgctggcaat gtccaagatc ctgaagaccc accccaagtc cgtgcagtcc cacaaggacc
     3061 tcatcctgca gtgcctggac gacaaggacg agtccatccg gctgcgggcc ctggacctgc
     3121 tctatgggat ggtgtccaag aagaacctga tggagatcgt gaagaagctg atgacccacg
     3181 tagacaaggc agagggtacc acctaccgtg acgagctgct caccaagatc attgacatct
     3241 gcagccagtc caactaccag tacatcacca acttcgagtg gtacatcagc atcctggtgg
     3301 agctgacccg gctggagggc acacggcacg gccacctcat cgccgcccaa atgctggacg
     3361 tggccatccg cgtgaaggcc atccgcaagt tcgccgtgtc ccagatgtct gcgctgcttg
     3421 acagtgcaca cctgctggcc agcagcaccc agcggaacgg gatctgtgag gtgctgtacg
     3481 ctgccgcctg gatctgcggg gagttctcag agcatctgca ggaaccacac cacactttgg
     3541 aggccatgct gcggcccaga gtcaccacgc tgccaggcca catccaggcc gtgtatgtgc
     3601 agaacgtggt caagctctac gcctccatcc tgcagcagaa ggagcaggcc ggggaggcag
     3661 agggcgctca ggccatcacc cagctcatgg tggaccggct gccccagttt gtgcagagcg
     3721 cagacctgga ggtgcaggag cgggcgtcct gcatcctgca gctggtcaag cacatccaga
     3781 agcttcaggc caaggacgtg cctgtggcag aggaggtcag cgctctcttt gctggggagc
     3841 tgaacccagt ggcccccaag gcccagaaga aggttccagt ccccgaaggc ctggacctgg
     3901 acgcctggat caatgagcca ctctcggaca gcgagtcaga ggacgagagg cccagggccg
     3961 tcttccacga ggaggagcag cggcgtccca agcaccggcc gtcggaggcg gacgaggaag
     4021 agctggctcg gcgccgagag gcccggaagc aggagcaggc caacaacccc ttctacatca
     4081 agagctcgcc atcgccacag aagcggtacc aggacacccc gggcgtggag cacattcccg
     4141 tggtgcagat tgacctctcc gtccccttga aggttccagg gctgcctatg tcagatcagt
     4201 atgtgaagct ggaggaggag cggcggcacc ggcagaagct ggagaaggac aagaggagga
     4261 aaaagaggaa ggagaaggag aagaagggca agcgccgcca cagctcgctg cccacggaga
     4321 gcgacgagga catcgcccct gcccagcagg tggacatcgt cacagaggag atgcctgaga
     4381 atgctctgcc cagcgacgag gatgacaaag accccaacga cccctacagg gctctggata
     4441 ttgacctgga taagccctta gccgacagcg agaaactgcc tattcagaaa cacagaaaca
     4501 ccgagacctc aaaatcccct gagaaggacg ttcccatggt agaaaagaag agcaagaaac
     4561 ccaagaagaa agagaaaaaa cacaaagaga aagagagaga caaggagaag aagaaggaga
     4621 aggagaagaa aaaatctccc aagcctaaga agaagaagca caggaaggag aaggaggagc
     4681 ggaccaaagg caagaagaag tccaagaagc agcctccagg cagcgaggag gcagcggggg
     4741 agccggtgca gaatggcgcg ccagaggagg agcagctccc gcctgagtcc agctactccc
     4801 tcctcgctga aaattcctat gttaaaatga cctgtgacat ccggggcagt ctgcaggagg
     4861 acagccaggt cactgtggcc atcgtgctgg agaacaggag cagcagcatc ctcaagggca
     4921 tggagctcag cgtgctggac tcactcaatg ccaggatggc ccggccgcag ggctcctccg
     4981 tccacgatgg cgtccccgtg cctttccagc tgcccccagg cgtctccaac gaagcccagt
     5041 atgtgttcac catccagagc atcgtcatgg cgcagaagct caaggggacc ctgtccttca
     5101 ttgccaagaa tgacgagggt gcgacccacg agaagctgga cttcaggctg cacttcagct
     5161 gcagctccta cttgatcacc actccctgct acagtgacgc ctttgctaag ttgctggagt
     5221 ctggggactt gagcatgagc tcaatcaaag tcgatggcat tcggatgtcc ttccagaatc
     5281 ttctggcgaa gatctgtttt caccaccatt tttccgttgt ggagcgagtg gactcctgtg
     5341 cctccatgta cagccgctcc atccagggcc accatgtctg cctcctggtg aaaaagggtg
     5401 agaactctgt ctcagtcgac gggaagtgca gtgactccac gctactgagc aacttgttag
     5461 aagagatgaa ggcgacgctg gccaagtgtt gactcgagct gcagatgaat cgtagatact
     5521 gaaaaacccc gcaagttcac ttcaactgtg catcgtgcac catctcaatt tctttcattt
     5581 atacatcgtt ttgccttctt ttatgtaact atactcctct aagtttcaat cttggccatg
     5641 taacctctga tctatagaat tttttaaatg actagaatta atgcccatct tttttttgga
     5701 cctaaattct tcatgaaaat atattacgag ggcttattca gaagctttgg acttcttcgc
     5761 cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta
     5821 cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc
     5881 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
     5941 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
     6001 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
     6061 taccggccgg tcgaaattcc cctaccctat gaacatattc cattttgtaa tttcgtgtcg
     6121 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
     6181 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
     6241 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
     6301 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
     6361 aagatagtgg cgatagggtt gaccttattc tttggcaaat ctggagcaga accgtggcat
     6421 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
     6481 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     6541 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     6601 gaatcaatca attgatgttg aaccttcaat gtaggaaatt cgttcttgat ggtttcctcc
     6661 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     6721 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     6781 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     6841 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     6901 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     6961 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     7021 ctacctgtac cccatttagg accacccaca gcacctaaca aaacggcatc agccttcttg
     7081 gaggcttcca gcgcctcatc tggaagtggg acacctgtag cttcgatagc agcaccacca
     7141 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     7201 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     7261 ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata ttgctgaaat
     7321 gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa
     7381 taggtcctta aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac
     7441 gcttctctat tctatatgaa aagccggttc cggcgctctc acctttcctt tttctcccaa
     7501 tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa aaaatttcca
     7561 gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa
     7621 aaataatggt tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca
     7681 gttgggggat ctcgactcta gctagaggat caattcgtaa tcatgtcata gctgtttcct
     7741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7861 gctttccagt cgggaaacct gtcgtgccag ctgataactt cgtataatgt atgctatacg
     7921 aagttattag gtctgaagag gagtttacgt ccagccaagc tagcttggct gcaggtcgag
     7981 cggccgcgat ccggaaccct taatataact tcgtataatg tatgctatac gaagttatca
     8041 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8101 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8161 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8701 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10021 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10081 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10141 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10201 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10261 gattgtactg agagtgcacc ataacgcatt taagcataaa cacgcactat gccgttcttc
    10321 tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt
    10381 atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc
    10441 ctattccgaa gttcctattc tctagctaga aagtatagga acttcagagc gcttttgaaa
    10501 accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag
    10561 ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat
    10621 atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc
    10681 atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa
    10741 aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc
    10801 ctatacgtag tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa
    10861 gaatcatcaa cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa
    10921 tataatcggg gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta
    10981 aacgcgggaa gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct
    11041 tcttctaacc ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg
    11101 cacaaaggag aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg
    11161 catttttgta gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt
    11221 tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg
    11281 cgttgcattt ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt
    11341 cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc
    11401 tctcgcgttg catttttgtt ctacaaaatg aagcacagat gcttcgttgc t
//
