LOCUS       sequence-387017-        9773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter: 
            p35S-PhiC31-NLS-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P22
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    255..277
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    285..461
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(693..1718)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1738..2083)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2286..2308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2300..2317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2301..2317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2324..2343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2324..2342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3340..3685
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3776..5593
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     misc_feature    6148..6172
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6263..6851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6343..6362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8360..9175)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8360..9175)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9083..9102
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9167..9186
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9466..9773,1..128)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc ccttggcttg
       61 tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg
      121 tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta taaatttttt
      181 tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga tttccgatcc
      241 ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg gattttagta
      301 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
      361 acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt
      421 tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga gatagatttg
      481 tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg cgaccggccc
      541 tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc caccaagcgt
      601 aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct gaggcgttct
      661 cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg
      721 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
      781 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
      841 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
      901 tggtcaagac caatgcggag catatacgcc cggagccttg gcgatcctgc aagctccgga
      961 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     1021 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     1081 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     1141 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     1201 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     1261 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     1321 aacccgctcg tctggctaag atcggccgca gcgatagcat ccatagcctc cgcgaccggc
     1381 tgaagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     1441 cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac ttccggaatc
     1501 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     1561 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     1621 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     1681 ttctcgacag acgtcgcggt gagttcaggc tttttcatag gggggatcag cttgggctgt
     1741 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     1801 ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct ttgwagacgt
     1861 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     1921 actgtcggca gaggcatctt gaatgatagc ctttccttta tcgcaatgat ggcatttgta
     1981 ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg ggcaatggaa
     2041 tccgaggagg tttcccgaaa ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     2101 tgagactgta tctttgacat ttttggagta ggggtacgat aacattaacg cttacaattt
     2161 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2221 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2281 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     2341 gggcttggag gaattccaat cccacaaaaa tctgagctta acagcacagt tgctcctctc
     2401 agagcagaat cgggtattca acaccctcat atcaactact acgttgtgta taacggtcca
     2461 catgccggta tatacgatga ctggggttgt acaaaggcgg caacaaacgg cgttcccgga
     2521 gttgcacaca agaaatttgc cactattaca gaggcaagag cagcagctga cgcgtacaca
     2581 acaagtcagc aaacagacag gttgaacttc atccccaaag gagaagctca actcaagccc
     2641 aagagctttg ctaaggccct aacaagccca ccaaagcaaa aagcccactg gctcacgcta
     2701 ggaaccaaaa ggcccagcag tgatccagcc ccaaaagaga tctcctttgc cccggagatt
     2761 acaatggacg atttcctcta tctttacgat ctaggaagga agttcgaagg tgaaggtgac
     2821 gacactatgt tcaccactga taatgagaag gttagcctct tcaatttcag aaagaatgct
     2881 gacccacaga tggttagaga ggcctacgca gcaagtctca tcaagacgat ctacccgagt
     2941 aacaatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     3001 aggactaatt gcatcaagaa cacagagaaa gacatatttc tcaagatcag aagtactatt
     3061 ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc
     3121 tctaaaaagg tagttcctac tgaatctaag gccatgcatg gagtctaaga ttcaaatcga
     3181 ggatctaaca gaactcgccg tcaagactgg cgaacagttc atacagagtc ttttacgact
     3241 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctgg tctactccaa
     3301 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat
     3361 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     3421 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
     3481 tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3541 ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccac
     3601 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     3661 aagttcattt catttggaga ggacacgctc gagtataaga gctcattttt acaacaatta
     3721 ccaacaacaa caaacaacaa acaacattac aattacattt acaattatcg atacaatgga
     3781 cacgtatgcc ggcgcttatg atagacaatc aagagaaaga gagaactctt ctgccgcctc
     3841 tccagctaca cagagatctg ccaacgagga taaagctgct gatttacaaa gagaggtaga
     3901 gagagatggg ggtaggttta gatttgtggg tcatttctct gaggctcctg gtacatcagc
     3961 ctttggtact gctgaacggc ctgaatttga aagaatatta aatgagtgtc gggccggtcg
     4021 actaaatatg ataatagttt atgatgtatc taggttcagt agactcaaag ttatggatgc
     4081 aattccgatc gtgagcgagc tacttgcact cggtgttact atcgtaagta cacaagaagg
     4141 tgtattcagg cagggtaatg tgatggacct tatacacctt attatgcgac tcgacgctag
     4201 ccataaagag tcatcactga agagtgctaa aatcttggac accaagaacc tgcaaaggga
     4261 gcttggaggc tatgtaggag gaaaagctcc gtatggtttc gaactcgtga gtgagacaaa
     4321 agagattacc agaaatggta ggatggtgaa cgtggttatt aataagctag ctcatagcac
     4381 aacccctctc acagggcctt ttgagttcga acctgacgtt attagatggt ggtggagaga
     4441 gatcaaaact cacaaacatc ttccatttaa gccaggtagc caggctgcta tccaccctgg
     4501 atctatcact gggctttgta aaaggatgga tgctgacgca gtgccaactc gaggggaaac
     4561 aatcggtaag aagactgcat cttccgcttg ggatcccgct acagttatga gaatccttcg
     4621 ggatccgcgt attgctggat tcgccgcaga agtcatttat aagaaaaaac ctgatggtac
     4681 tcctactaca aaaatcgaag gttataggat ccaaagggat cctattactt tgcgtccagt
     4741 tgaattagac tgtggaccga tcatcgaacc tgccgagtgg tacgagttgc aggcctggct
     4801 tgatggtaga gggcgtggaa agggtttatc tcggggacag gcaattttgt cagcaatgga
     4861 taagctttat tgtgagtgtg gggctgttat gacatcaaaa cgtggagaag agtcaatcaa
     4921 ggattcttac aggtgcagac gtcgtaaagt ggtggaccct tctgctccag gacagcatga
     4981 gggtacatgc aacgtttcta tggctgctct tgataaattt gtggccgaac gtattttcaa
     5041 caagattaga cacgctgaag gggatgagga aactttagct cttctttggg aggctgcccg
     5101 gcgttttggt aagctaacag aggcaccaga aaaaagtggc gaaagagcta atttggtagc
     5161 cgaaagagca gacgccttga atgcattgga agaactttac gaagataggg ctgccggagc
     5221 atatgatgga ccagttggtc ggaagcattt caggaagcaa caagctgcac taactttgag
     5281 acagcaaggc gcagaggagc gactcgcaga gcttgaggcc gccgaggccc caaagcttcc
     5341 actcgatcaa tggtttcctg aggatgccga cgctgatcct acaggtccaa agagctggtg
     5401 gggaagagct agcgtagacg ataagagagt gtttgtgggt ctatttgttg acaagattgt
     5461 agttactaag tccactacgg gtcgaggaca gggcactccc atcgaaaaga gagccagtat
     5521 tacctgggca aagcctccaa ccgatgatga tgaagacgat gcacaagatg gtaccgagga
     5581 tgtggcagca taataagctt agagactctt atcaagaatc ccatctcttg cttgcttttt
     5641 tttgttgtct tccctttgat agggtttgtt tttcttgttt cagtgacttt ctatgttaaa
     5701 agataatgtc agtaaaagga tttggttttc tattattctg aatcgattac ggaagattct
     5761 tgcttaattc caatctatac aagtatcgtg aaataatgac cgtttatgtg attaggagac
     5821 gtgtttcatt aataaaatat aagatcaata cattgttagt agtgataaac tatgtacaaa
     5881 ttgtattgat tgtaaaagaa acacaatagg ttcctttttt ctacaatata ttgtgacaga
     5941 ctctctgttt taacgaatga attaaatttg tcaacgaaaa aattaaagaa tgatgtgatc
     6001 actttgcacc ttggttcatc ttcagaggct tctgcatgtt ccatgcattg aggctctcta
     6061 cggtcacagg ttgtgagccg ttgttaaaga caaacaaatg cgctaccaca gaatcagggg
     6121 ataacgcagg aaagaacatg aaggccttga caggatatat tggcgggtaa actaagtcgc
     6181 tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6721 gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac
     6781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6961 taaattaaaa atgaagtttt aaatcaatct aaagtactga gagatcccct cataatttcc
     7021 ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt
     7081 cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg
     7141 agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct
     7201 gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga
     7261 tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac
     7321 ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt
     7381 gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc tcctttgccg
     7441 acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt
     7501 ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag
     7561 tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga
     7621 taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta
     7681 accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac
     7741 tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt
     7801 catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag
     7861 aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc tcacgaatat
     7921 cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc
     7981 aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc
     8041 gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag
     8101 tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg agtcgtttta
     8161 agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt tcgcacatct
     8221 tgttgtctga ttattgattt ttcgcgaaac catttgatca tatgacaaga tgtgtatcca
     8281 ccttaactta atgattttta ccaaaatcat taggggattc atcagtctaa agtatatatg
     8341 tgtaacattg gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     8401 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     8461 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     8521 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     8581 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     8641 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     8701 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     8761 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     8821 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
     8881 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     8941 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     9001 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg
     9061 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     9121 tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac
     9181 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     9241 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     9301 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     9361 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     9421 gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg
     9481 ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga
     9541 aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta
     9601 tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct
     9661 aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca
     9721 gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agt
//
