LOCUS       sequence-387018-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-Delta-N-CHMP4C(S191C,I225A,L228A,W231A)
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..186
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             187..198
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             238..255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             256..276
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcagtata aactggcgct gaacggcaaa accctgaaag gcgaaaccac caccgaagcg
       61 gtggatgcgg cgaccgcgga aaaagtgttt aaacagtatg cgaacgataa cggcgtggat
      121 ggcgaatgga cctatgatga tgcgaccaaa acctttaccg tgaccgaact ggtgccgcgc
      181 ggcagcattg aaggccgcat tcgcgaaaac ctgaaagata gcggcctgtt tctggaacat
      241 catcatcatc atcatgaaaa cctgtatttt caaggcagcg aaaacatgga tctgaacaaa
      301 attgatgatc tgatgcaaga aattaccgaa cagcaagata ttgcgcaaga aattagcgaa
      361 gcgtttagtc agcgcgtggg ctttggcgat gattttgatg aagatgaact gatggcggaa
      421 ctggaagaac tggaacaaga agaactgaac aaaaaaatga ccaatattcg cctgccgaac
      481 gttccgagct gcagcctgcc ggctcagccg aaccgcaaac cgggcatgag cagcactgcg
      541 cgtcgctcgc gggcggcgag cagtcagcgc gcggaagaag aagatgatga tgccaaacag
      601 gcggcggcgg cggcgaccta a
//
