LOCUS       sequence-387022-       10530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with constitutive promoter: 
            pUBQ10-PhiC31-NLS-DST-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P19
FEATURES             Location/Qualifiers
     source          1..10530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    257..279
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    287..463
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(695..1720)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1740..2085)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1777..1797)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2288..2310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2302..2319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2303..2319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2326..2345
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2326..2344
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4380..6194
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             6195..6215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6907..6931
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7022..7610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7102..7121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9119..9934)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9119..9934)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9842..9861
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9926..9945
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(10225..10530,1..130)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc gcccttggct
       61 tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc ggttgcccac
      121 cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt tataaatttt
      181 tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg gatttccgat
      241 ccccggaatt agatcttggc aggatatatt gtggtgtaac gttatcgatc tggattttag
      301 tactggattt tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa
      361 atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata agaaccctaa
      421 tttcccttat cgggaaacta ctcacacatt atttatggag aaaatagaga gagatagatt
      481 tgtagagaga gactggtgat ttcagcgtac cgaattaatt cctgaatcac tgcgaccggc
      541 cctcccgcga cccagccgag cgagcttagc gaactgtgga cgagaactgt gccaccaagc
      601 gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt
      661 ctcgaaatca gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
      721 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
      781 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
      841 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
      901 gttggtcaag accaatgcgg agcatatacg cccggagcct tggcgatcct gcaagctccg
      961 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     1021 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     1081 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     1141 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     1201 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     1261 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     1321 cgaacccgct cgtctggcta agatcggccg cagcgatagc atccatagcc tccgcgaccg
     1381 gctgaagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     1441 ggcgggagat gcaataggtc aggctctcgc tgaactcccc aatgtcaagc acttccggaa
     1501 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     1561 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     1621 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     1681 acttctcgac agacgtcgcg gtgagttcag gctttttcat aggggggatc agcttgggct
     1741 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     1801 gattgtgcgt catcccttac gtcagtggag atgtcacatc aatccacttg ctttgaagac
     1861 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     1921 ccactgtcgg cagaggcatc ttgaatgata gcctttcctt tatcgcaatg atggcatttg
     1981 taggagccac cttccttttc tactgtcctt tcgatgaagt gacagatagc tgggcaatgg
     2041 aatccgagga ggtttcccga aattaccctt tgttgaaaag tctcaatagc cctttggtct
     2101 tctgagactg tatctttgac atttttggag taggggtacg ataacattaa cgcttacaat
     2161 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     2221 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2281 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     2341 tagggcttgg agctcaacaa caaactttcc attcacccat ttatggatac acagtctagc
     2401 tcaacagagc ttttaaccca aattggtaca atagaataca actttagatc ataattctca
     2461 aaagaaagag attccttagc tattctatct gccactccat ttccttctcg gcttgtatgc
     2521 acaagcataa aatcctcaaa cttgctaagt agatacttta tgtcttggat aattggattg
     2581 agacttgaca agcataactt tcatgtaacc aaagacacaa gttgctgaga atccacctca
     2641 aaaatgatct tcctataatt gaatcgggat aatgacagca cagcccatct aagagcctcc
     2701 acttctactt ccagcacgct tcttactttt accacagctc ttgcacctaa ccataacacc
     2761 ttccctgtat gatcgcgaag cacccaccct aagccacatt ttaatccttc tgttggccat
     2821 gccccatcaa agttgcactt aacccaagat tgtggtggag cttcccatgt ttctcgtctg
     2881 tcccgacggt gttgtggttg gtgctttcct tacattctga gcctctttcc ttctaatcca
     2941 ctcatctgca tcttcttgtg tccttactaa tacctcattg gttccaaatt ccctcccttt
     3001 aagcaccagc tcgtttctgt tcttccacag cctcccaagt atccaaggga ctaaagcctc
     3061 cacattcttc agatcaggat attcttgttt aagatgttga actctatgga ggtttgtatg
     3121 aactgatgat ctaggaccgg ataagttccc ttcttcatag cgaacttatt caaagaatgt
     3181 tttgtgtatc attcttgtta cattgttatt aatgaaaaaa tattattggt cattggactg
     3241 aacacgagtg ttaaatatgg accaggcccc aaataagatc cattgatata tgaattaaat
     3301 aacaagaata aatcgagtca ccaaaccact tgcctttttt aacgagactt gttcaccaac
     3361 ttgatacaaa agtcattatc ctatgcaaat caataatcat acaaaaatat ccaataacac
     3421 taaaaaatta aaagaaatgg ataatttcac aatatgttat acgataaaga agttactttt
     3481 ccaagaaatt cactgatttt ataagcccac ttgcattaga taaatggcaa aaaaaaacaa
     3541 aaaggaaaag aaataaagca cgaagaattc tagaaaatac gaaatacgct tcaatgcagt
     3601 gggacccacg gttcaattat tgccaatttt cagctccacc gtatatttaa aaaataaaac
     3661 gataatgcta aaaaaatata aatcgtaacg atcgttaaat ctcaacggct ggatcttatg
     3721 acgaccgtta gaaattgtgg ttgtcgacga gtcagtaata aacggcgtca aagtggttgc
     3781 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     3841 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     3901 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     3961 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     4021 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     4081 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     4141 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     4201 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     4261 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt
     4321 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagaa
     4381 tggacacgta tgccggcgct tatgatagac aatcaagaga aagagagaac tcttctgccg
     4441 cctctccagc tacacagaga tctgccaacg aggataaagc tgctgattta caaagagagg
     4501 tagagagaga tgggggtagg tttagatttg tgggtcattt ctctgaggct cctggtacat
     4561 cagcctttgg tactgctgaa cggcctgaat ttgaaagaat attaaatgag tgtcgggccg
     4621 gtcgactaaa tatgataata gtttatgatg tatctaggtt cagtagactc aaagttatgg
     4681 atgcaattcc gatcgtgagc gagctacttg cactcggtgt tactatcgta agtacacaag
     4741 aaggtgtatt caggcagggt aatgtgatgg accttataca ccttattatg cgactcgacg
     4801 ctagccataa agagtcatca ctgaagagtg ctaaaatctt ggacaccaag aacctgcaaa
     4861 gggagcttgg aggctatgta ggaggaaaag ctccgtatgg tttcgaactc gtgagtgaga
     4921 caaaagagat taccagaaat ggtaggatgg tgaacgtggt tattaataag ctagctcata
     4981 gcacaacccc tctcacaggg ccttttgagt tcgaacctga cgttattaga tggtggtgga
     5041 gagagatcaa aactcacaaa catcttccat ttaagccagg tagccaggct gctatccacc
     5101 ctggatctat cactgggctt tgtaaaagga tggatgctga cgcagtgcca actcgagggg
     5161 aaacaatcgg taagaagact gcatcttccg cttgggatcc cgctacagtt atgagaatcc
     5221 ttcgggatcc gcgtattgct ggattcgccg cagaagtcat ttataagaaa aaacctgatg
     5281 gtactcctac tacaaaaatc gaaggttata ggatccaaag ggatcctatt actttgcgtc
     5341 cagttgaatt agactgtgga ccgatcatcg aacctgccga gtggtacgag ttgcaggcct
     5401 ggcttgatgg tagagggcgt ggaaagggtt tatctcgggg acaggcaatt ttgtcagcaa
     5461 tggataagct ttattgtgag tgtggggctg ttatgacatc aaaacgtgga gaagagtcaa
     5521 tcaaggattc ttacaggtgc agacgtcgta aagtggtgga cccttctgct ccaggacagc
     5581 atgagggtac atgcaacgtt tctatggctg ctcttgataa atttgtggcc gaacgtattt
     5641 tcaacaagat tagacacgct gaaggggatg aggaaacttt agctcttctt tgggaggctg
     5701 cccggcgttt tggtaagcta acagaggcac cagaaaaaag tggcgaaaga gctaatttgg
     5761 tagccgaaag agcagacgcc ttgaatgcat tggaagaact ttacgaagat agggctgccg
     5821 gagcatatga tggaccagtt ggtcggaagc atttcaggaa gcaacaagct gcactaactt
     5881 tgagacagca aggcgcagag gagcgactcg cagagcttga ggccgccgag gccccaaagc
     5941 ttccactcga tcaatggttt cctgaggatg ccgacgctga tcctacaggt ccaaagagct
     6001 ggtggggaag agctagcgta gacgataaga gagtgtttgt gggtctattt gttgacaaga
     6061 ttgtagttac taagtccact acgggtcgag gacagggcac tcccatcgaa aagagagcca
     6121 gtattacctg ggcaaagcct ccaaccgatg atgatgaaga cgatgcacaa gatggtaccg
     6181 aggatgtggc agcaccaaaa aaaaagagga aggtgtaagc ttagtactat actacaacat
     6241 ttccatattt tttttagatt gttagctaat ttcccctgga gataattgta aattgtttca
     6301 atgagaggaa tatacaatac atagatcgta attgatcaat gcgtatttgc atgttggtaa
     6361 gagactctta tcaagaatcc catctcttgc ttgctttttt ttgttgtctt ccctttgata
     6421 gggtttgttt ttcttgtttc agtgactttc tatgttaaaa gataatgtca gtaaaaggat
     6481 ttggttttct attattctga atcgattacg gaagattctt gcttaattcc aatctataca
     6541 agtatcgtga aataatgacc gtttatgtga ttaggagacg tgtttcatta ataaaatata
     6601 agatcaatac attgttagta gtgataaact atgtacaaat tgtattgatt gtaaaagaaa
     6661 cacaataggt tccttttttc tacaatatat tgtgacagac tctctgtttt aacgaatgaa
     6721 ttaaatttgt caacgaaaaa attaaagaat gatgtgatca ctttgcacct tggttcatct
     6781 tcagaggctt ctgcatgttc catgcattga ggctctctac ggtcacaggt tgtgagccgt
     6841 tgttaaagac aaacaaatgc gctaccacag aatcagggga taacgcagga aagaacatga
     6901 aggccttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc
     6961 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7441 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7501 ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7741 aatcaatcta aagtactgag agatcccctc ataatttccc caaagcgtaa ccatgtgtga
     7801 ataaattttg agctagtagg gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc
     7861 cagaatgccg caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga
     7921 ctgtgttagc ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc
     7981 aacatgttag ttgaagcatc agggcgatta gcagcatgat atcaaaacgc tctgagctgc
     8041 tcgttcggct atggcgtagg cctagtccgt aggcaggact tttcaagtct cggaaggttt
     8101 cttcaatctg cattcgcttc gaatagatat taacaagttg tttgggtgtt cgaatttcaa
     8161 caggtaagtt agttgctaga acccatggct cctttgccga cgctgagtag attttaggtg
     8221 acgggtggtg acaatgagtc cgtgtcgagc gctgattttt tcggccttta gagcgagatt
     8281 tatacaatag aatttggcat gagattggat tgcttttagt cagcctctta tagcctaaag
     8341 tctttgagtg actagatgac atatcatgta agttgctgat aggtttccag ttttccgctc
     8401 ctaggtctgc atattgtact tttcctctta ctcgacttaa ccagtaccaa cccagcttct
     8461 caacggattt ataccatggc actttaaagc cagcatcact gacaatgagc ggtgtggtgt
     8521 tactcggtag aatgctcgca aggtcggcta gaaattggtc atgagctttc tttgaacatt
     8581 gctctgaaag cgggaacgct ttctcataaa gagtaacaga acgaccgtgt agtgcgactg
     8641 aagctcgcaa taccataagt cgtttttgct cacgaatatc agaccagtca acaagtacaa
     8701 tgggcatcgt attgcccgaa cagataaagc tagcatgcca acggtataca gcgagtcgct
     8761 ctttgtggag gtgacgatta cctaacaatc ggtcgattcg tttgatgtta tgttttgttc
     8821 tcgctttggt tggcaggtta cggccaagtt cggtaagagt gagagtttta cagtcaagta
     8881 atgcgtggca agccaacgtt aagctgttga gtcgttttaa gtgtaattcg gggcagaatt
     8941 ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatctt gttgtctgat tattgatttt
     9001 tcgcgaaacc atttgatcat atgacaagat gtgtatccac cttaacttaa tgatttttac
     9061 caaaatcatt aggggattca tcagtctaaa gtatatatgt gtaacattgg tctagtgatt
     9121 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     9181 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     9241 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     9301 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     9361 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     9421 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     9481 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     9541 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     9601 cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat
     9661 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     9721 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     9781 actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat
     9841 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     9901 ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     9961 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
    10021 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
    10081 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
    10141 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
    10201 atgatggggc gattcaggcg atccccatcc aacagcccgc cgtcgagcgg gcttttttat
    10261 ccccggaagc ctgtggatag agggtagtta tccacgtgaa accgctaatg ccccgcaaag
    10321 ccttgattca cggggctttc cggcccgctc caaaaactat ccacgtgaaa tcgctaatca
    10381 gggtacgtga aatcgctaat cggagtacgt gaaatcgcta ataaggtcac gtgaaatcgc
    10441 taatcaaaaa ggcacgtgag aacgctaata gccctttcag atcaacagct tgcaaacacc
    10501 cctcgctccg gcaagtagtt acagcaagta
//
