LOCUS       sequence-387043-       11400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-4 
            epsilon fusion protein in yeast (yeast two-hybrid or yeast 
            three-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Mattera R, Boehm M, Chaudhuri R, Prabhu Y, Bonifacino JS
  TITLE     Conservation and diversification of dileucine signal recognition by 
            adaptor protein (AP) complex variants.
  JOURNAL   J Biol Chem. 2011 Jan 21;286(3):2022-30. doi: 
            10.1074/jbc.M110.197178. Epub 2010 Nov 19.
  PUBMED    21097499
REFERENCE   2  (bases 1 to 11400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.197178"; journalName: "J Biol Chem"; date: 
            "2011-01-21- 21"; volume: "286"; issue: "3"; pages: "2022-30"
COMMENT     Sequence Label: pGADT7-epsilon
FEATURES             Location/Qualifiers
     source          1..11400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1971..2003
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             2013..2027
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     RBS             4299..4310
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5828..6015
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5883..5907)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(6132..7226)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7227..7632)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7674..7690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7674..7690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7687..7709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7698..7714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7722..7728,7729..7746,7747..7752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7767..7788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7843..7876
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7954..7987
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8056..8073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8227..8815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8307..8326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8986..9777,9778..9846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9609..9628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9847..9951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10019..10037
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10075..10097)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10197..10216
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      10233..11397
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggctagca
     1981 tgactggtgg acagcaaatg ggtcgcggat ctgacgacga cgacaagatg agcgacatag
     2041 tggagaagac gctgacggcg ctgccgggac tctttctgca gaaccagccc ggtggtgggc
     2101 ccgcggccgc caaggcgtcc ttctcctcga ggctgggcag ccttgtccgc ggcatcacag
     2161 ccctcacctc caagcacgaa gaagaaaaat taatccagca ggaactgagt agtctgaaag
     2221 cgactgtttc tgctcctact acaacactga aaatgatgaa ggaatgtatg gtgagactta
     2281 tatattgtga aatgcttgga tatgatgctt cctttggcta tattcatgca atcaagttag
     2341 cccaacaagg aaacctctta gaaaaaagag taggttattt ggctgtttcc ttatttctac
     2401 atgaaagtca tgaattattg cttctccttg tgaatacagt tgtaaaggat ctgcagagca
     2461 ctaacctagt agaagtgtgt atggcactga ctgttgttag ccagattttc ccctgcgaaa
     2521 tgattccagc tgttcttcca ttaatagaag ataaacttca acattctaag gagattgtac
     2581 gaagaaaagc tgttctggca ttatacaaat tccatctcat tgctcctaat caagtacaac
     2641 atattcatat taagtttcgg aaagcacttt gtgacagaga tgttggggtc atggctgcct
     2701 ccttgcatat atatcttaga atgattaagg agaattcatc tggatataaa gacttgactg
     2761 ggagttttgt aaccattttg aagcaagtag ttggaggaaa gctcccagta gaattcaatt
     2821 accacagtgt gccagcacca tggttacaaa ttcagctctt gagaatactg ggacttctag
     2881 gaaaagatga tcaaaggaca agtgaattaa tgtatgatgt tcttgatgaa tccttacgaa
     2941 gagctgagtt aaatcacaat gtcacatatg ctattttgtt tgaatgtgtg catacagtct
     3001 attctattta tcctaaatcg gaattacttg agaaggctgc caagtgcatt ggaaaatttg
     3061 ttctgtcacc taaaataaat ctaaaatatt taggactgaa ggctcttacc tatgttatcc
     3121 aacaggatcc tactctggct cttcaacacc agatgacaat aattgaatgt ttagatcatc
     3181 ctgatcccat tattaaaaga gagactctgg aacttcttta cagaattact aatgcacaga
     3241 atataacagt tattgtccag aaaatgcttg aatatttaca tcagagcaaa gaagagtatg
     3301 tcatcgtcaa tttggtcggc aaaatagcag agctggctga gaaatatgct cctgataatg
     3361 catggtttat tcagacaatg aatgctgtgt tttcagtagg aggagatgta atgcatcctg
     3421 atattcccaa taactttctg agactactag cggaaggttt tgatgatgaa acagaagatc
     3481 agcaattaag actctatgca gttcagtctt atctcacttt actggatatg gaaaatgtgt
     3541 tctatccaca gagatttctt caagttatga gttgggtatt aggggaatat tcctacctct
     3601 tagataagga aacgccagag gaagttatag ctaagctcta caagttactt atgaatgact
     3661 ctgtgtcttc agaaacaaaa gcctggttaa ttgctgctgt gaccaaattg acatctcagg
     3721 cgcactcttc taatacagtt gagagattaa tccatgaatt taccatatct ttggatactt
     3781 gtatgagaca acatgcattt gaattaaaac atttgcatga gaatgtggaa cttatgaaga
     3841 gcttgcttcc agttgacagg agttgtgaag acttggtggt agatgcttct ttatcttttc
     3901 tggatggttt tgtggctgaa ggactcagtc agggtgcagc gccttacaaa cctccccatc
     3961 aacgccagga ggaaaagctt tctcaggaaa aagttctcaa ttttgaacca tatggactct
     4021 ccttttcttc atctggcttc actggacgac agtctcctgc tggcatttct cttggttcag
     4081 atgtatctgg gaatagtgct gagacaggac tgaaagagac aaatagcttg aagctggaag
     4141 gtataaagaa attgtggggg aaagaaggct atcttcccaa gaaggaaagc aaaactggtg
     4201 atgaaagtgg agctctgcct gttcctcaag agagtataat ggagaatgta gatcaagcta
     4261 taactaaaaa ggatcaatct caagttctta cccaatctaa agaggagaaa gaaaagcagc
     4321 tgctggcatc atcattattt gttggtctag gatcagaaag tacaatcaac ctgctgggaa
     4381 aagcagatac tgtctctcac aagttcagaa ggaaatcaaa agtcaaagaa gctaaaagtg
     4441 gcgaaacaac cagtactcat aatatgacct gttcttcctt tagttctttg tcaaatgtgg
     4501 catatgaaga tgattattat tcgaatactt tgcacgatac aggagacaag gaattaaaga
     4561 aattttctct cacttcagaa cttttggatt ctgagtcact cacagaactg cccttggttg
     4621 agaaattctc atattgtagt ctgtctacac cttcattgtt tgctaataac aacatggaaa
     4681 tttttcaccc tcctcaatct actgcagcct cagttgccaa ggaaagctct ttagcttcat
     4741 cctttttgga agaaactact gaatacatac actcaaatgc tatggaagtc tgtaataatg
     4801 aaactatatc agtgtcttct tataaaattt ggaaagatga ttgtttattg atggtctggt
     4861 cagtcactaa taagagtggt ttggaattga aaagtgctga cttagaaatt tttcctgcag
     4921 aaaatttcaa ggtgactgag caacctggat gctgtttgcc tgtaatggaa gcagaaagca
     4981 ccaaaagctt tcaatatagt gtgcagatag aaaaaccttt tacagaagga aatcttactg
     5041 gttttattag ttatcatatg atggatactc attctgctca gctggaattt tctgtaaact
     5101 tatcactatt agatttcatt agaccattaa aaatctcaag tgacgacttt gggaaactct
     5161 ggttatcctt cgcaaatgat gtgaaacaaa atgtaaaaat gtcagaatct caagctgcac
     5221 ttccttctgc actaaagact ctgcaacaga aactaagact ccatattatt gagattatag
     5281 gcaatgaagg gctattggcc tgtcagctgc tcccatccat cccctgctta ctgcattgcc
     5341 gagttcatgc agatgtatta gccctgtggt tcagatcctc ctgttctact cttcctgact
     5401 atttactgta tcaatgtcaa aaggtgatgg agggatctta ggtcgagctg cagatgaatc
     5461 gtagatactg aaaaaccccg caagttcact tcaactgtgc atcgtgcacc atctcaattt
     5521 ctttcattta tacatcgttt tgccttcttt tatgtaacta tactcctcta agtttcaatc
     5581 ttggccatgt aacctctgat ctatagaatt ttttaaatga ctagaattaa tgcccatctt
     5641 ttttttggac ctaaattctt catgaaaata tattacgagg gcttattcag aagctttgga
     5701 cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc
     5761 agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc
     5821 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     5881 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     5941 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
     6001 ccacacctct accggccggt cgaaattccc ctaccctatg aacatattcc attttgtaat
     6061 ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata tcataaaaaa
     6121 agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc accgacttcg
     6181 gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa aaccttttta
     6241 actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt caacatcatt
     6301 gcagcagaca agatagtggc gatagggttg accttattct ttggcaaatc tggagcagaa
     6361 ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac
     6421 gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga gatgatatca
     6481 ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt aactaggatc
     6541 atggcggcag aatcaatcaa ttgatgttga accttcaatg taggaaattc gttcttgatg
     6601 gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc tttatccaag
     6661 gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat tcttgtgatt
     6721 ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc accatcgtct
     6781 tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac gaagtcagta
     6841 cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga tgcaaagtta
     6901 catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa accttgttca
     6961 ggtctaacac tacctgtacc ccatttagga ccacccacag cacctaacaa aacggcatca
     7021 gccttcttgg aggcttccag cgcctcatct ggaagtggga cacctgtagc ttcgatagca
     7081 gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac atcagaaata
     7141 gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc acctggcaaa
     7201 acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat atatatatat
     7261 tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac gattgctaac cacctattgg
     7321 aaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg caagcattta
     7381 gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcgctctca cctttccttt
     7441 ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa
     7501 aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt
     7561 tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt
     7621 attcccacag ttgggggatc tcgactctag ctagaggatc aattcgtaat catgtcatag
     7681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgataacttc gtataatgta
     7861 tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct agcttggctg
     7921 caggtcgagc ggccgcgatc cggaaccctt aatataactt cgtataatgt atgctatacg
     7981 aagttatcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8041 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8101 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8161 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8221 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8281 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8341 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8401 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8461 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8521 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8581 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8641 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8701 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8761 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8941 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9001 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9061 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9121 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9181 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9241 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9301 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9361 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9421 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9481 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9541 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9601 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9661 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9721 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9781 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9841 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9901 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9961 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10021 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10081 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10141 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10201 atcagagcag attgtactga gagtgcacca taacgcattt aagcataaac acgcactatg
    10261 ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca
    10321 gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct
    10381 ttgaagttcc tattccgaag ttcctattct ctagctagaa agtataggaa cttcagagcg
    10441 cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac
    10501 tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct
    10561 ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct
    10621 tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt
    10681 agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt
    10741 aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg
    10801 accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc
    10861 ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag
    10921 taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca
    10981 aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat
    11041 tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct
    11101 ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg
    11161 ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta
    11221 gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
    11281 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
    11341 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttgct
//
