LOCUS       sequence-387044-       14368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the CHD3 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0656
FEATURES             Location/Qualifiers
     source          1..14368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7306..7894
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7359..7379)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7777..7788)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7966..8199
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8277..8398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8314..8333)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8368..8387
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8438..8573
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8593..8612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8594..8612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8622..8639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8622..8638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8631..8653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8780..9235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8867..8886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9077..9098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9261..9365
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9366..9434,9435..10226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9584..9603)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10397..10985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10886..10905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11139..11156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11273..11294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11309..11314,11315..11332,11333..11339)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11347..11363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11352..11374
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11371..11387
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11371..11387
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11406..11426
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11408..11426
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11454..11680
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11681..11861
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11908..12033
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12526..12759
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12944..12988
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        13219..13429
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13241..13437
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13288..13423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13469..14071
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(13469..13488)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13965..13985
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14114..14231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ggcggcagac actgtgatcc tgtgggcaag aagtaaaaat gaccagctga ggatttcttt
     1261 tcctccagga ctgtgttggg gtgacaggat gcctgataag gatgacattc ggctgctgcc
     1321 gtcagcattg ggtgtgaaga agagaaaacg aggacccaag aagcagaagg agaacaagcc
     1381 aggaaaaccc cgaaaacgca agaagcgtga cagtgaggag gaatttggtt ctgagcgaga
     1441 tgagtaccgg gagaagtcag agagtggggg cagtgaatat ggaaccggac cgggtcggaa
     1501 acgaagaagg aagcaccgag aaaaaaagga gaagaagaca aagcggcgga aaaaggggga
     1561 gggagatggg gggcaaaagc aagtggaaca gaagtcatca gcaactctgc ttctgacctg
     1621 gggcctggag gatgtggagc atgtgttctc tgaggaggat taccacacgc tcaccaacta
     1681 caaagccttc agccagttca tgaggcccct aattgctaag aagaatccta agatcccaat
     1741 gtctaagatg atgaccatcc ttggggccaa atggagagag ttcagtgcca acaacccctt
     1801 caaggggtca gcagctgctg tggcggcggc agcggcagca gcagcagcag ctgtagctga
     1861 gcaggtgtca gctgctgtct cgtcggccac ccccatagca ccctccggac cccccgccct
     1921 tccaccaccc cctgctgctg atatccagcc cccacccatc cgaagagcca aaaccaaaga
     1981 gggcaaaggt ccaggccata agaggcggag taagagcccc cgagtgcctg atggacgcaa
     2041 gaagcttcgg ggaaagaaaa tggcaccact caaaataaaa ctagggcttc tgggtggcaa
     2101 gaggaagaaa ggaggctcgt atgtttttca gagcgacgaa ggtcctgaac cagaggctga
     2161 ggaatcagac ctggacagtg gcagtgtcca cagtgcctca ggccggcctg atggccctgt
     2221 ccgcaccaag aaactaaaga gaggccggcc aggaaggaag aagaagaagg tcctgggctg
     2281 tcctgcagtg gccggggagg aggaggttga tggctacgag acggatcacc aggattactg
     2341 tgaggtgtgc cagcagggtg gggaaattat tctgtgtgac acctgccctc gtgcctacca
     2401 cctcgtctgc cttgatcctg agcttgaccg ggctccagag ggcaaatgga gctgccctca
     2461 ctgtgagaag gagggggtcc agtgggaggc caaggaggaa gaagaagaat acgaagagga
     2521 gggagaggaa gaaggggaga aggaggagga ggatgatcac atggagtact gccgcgtatg
     2581 caaggacggc ggggagctcc tgtgctgtga cgcgtgcatc tcctcctacc acattcattg
     2641 tctaaaccct cccctgcctg acattcccaa tggtgaatgg ctgtgtcccc gatgcacatg
     2701 ccccgtgctg aagggtcgag tgcagaagat cctacattgg cggtgggggg agccacctgt
     2761 agcagtgcca gcccctcaac aggcagatgg aaatccagat gtcccacccc cccgtcctct
     2821 tcaaggcaga tcagagcgag agttctttgt caagtgggta ggactatcct actggcactg
     2881 ctcctgggcc aaggagcttc agctggaaat cttccatttg gttatgtatc gaaactacca
     2941 gcggaagaat gacatggatg agcccccacc cctggactat ggctccggcg aggatgatgg
     3001 gaagagcgac aagcgtaaag tgaaagaccc gcactatgct gagatggagg agaagtacta
     3061 tcgttttggc atcaagccag agtggatgac cgtccaccgc atcatcaacc acagtgtgga
     3121 taaaaagggg aattaccact atctagtaaa atggagggac ttaccatatg accagtccac
     3181 gtgggaggaa gatgaaatga atatccctga atacgaagaa cataagcaaa gctactggag
     3241 acaccgagaa ctaattatgg gggaagaccc tgcccagccc cgcaagtata agaagaagaa
     3301 gaaggagcta cagggtgatg ggcctcccag ttctcccact aatgatccta ccgtgaaata
     3361 tgagactcag ccacggttta tcacagccac tggaggcacc ctgcacatgt atcagttgga
     3421 agggctgaac tggctacgct tctcctgggc ccagggcact gacaccattc tagctgatga
     3481 gatggggcta ggcaagacca tacaaaccat cgtcttcctc tactcactct acaaggaggg
     3541 ccacacaaaa ggtcccttcc tggtgagtgc cccactctct accatcatta actgggagcg
     3601 ggagttccag atgtgggcac ccaaattcta tgtggtgaca tacacgggtg acaaggacag
     3661 ccgggccatc attcgtgaga atgaattctc ctttgaggac aatgccatca aagggggcaa
     3721 gaaagctttt aagatgaaga gggaggcaca ggtgaagttc catgttctcc tgacatcgta
     3781 tgagctgatc accattgatc aggcagcact tggttccatc cgctgggcct gtcttgtggt
     3841 agatgaggcc catcgactca agaacaacca gtccaagttt ttcagggttc tcaatggtta
     3901 caagatagat cataagttgc tgctgacagg aaccccattg cagaataatc tggaggagct
     3961 cttccatctc ctgaacttcc tcaccccaga gagatttaac aacttggagg gcttcctgga
     4021 ggagtttgct gacatatcca aagaggacca gatcaagaaa ctgcatgatt tgctggggcc
     4081 acacatgctg cggagactca aggcagatgt ctttaagaac atgccagcca agacagagct
     4141 catcgttcgg gtggagctaa gccccatgca gaagaaatac tacaaataca tcctgactcg
     4201 aaattttgag gccttgaatt cacgaggtgg tgggaaccag gtgtcgctgc ttaatatcat
     4261 gatggatctt aagaagtgct gcaaccatcc ataccttttt cccgtggctg ctatggagtc
     4321 ccccaaactc cccagtgggg cttatgaggg tggggcactt attaagtcgt ctgggaagct
     4381 catgctgctc cagaagatgc tgcgaaagct gaaggagcaa ggacaccgag tgctcatctt
     4441 ctcgcagatg accaaaatgt tagacttgct tgaggacttc ttagactatg aaggctacaa
     4501 gtatgagcgc atcgatggtg gtatcacggg tgccctgagg caggaggcca tcgatcggtt
     4561 taatgctcct ggggcccaac aattctgctt cctcctgtcc acccgagctg ggggcctggg
     4621 catcaatctg gccactgctg acactgtcat catctttgat tctgactgga acccccataa
     4681 tgacatccag gcctttagcc gggctcatcg gattggccag gccaacaaag tgatgattta
     4741 ccggtttgtg actcgcgcgt cagtggaaga gcgaatcaca caagtggcca agagaaagat
     4801 gatgctgaca cacctggttg tgcggcctgg gctgggctcc aaggcaggct ccatgtccaa
     4861 gcaggagctt gacgacattc tcaaatttgg cactgaagag ctattcaagg atgaaaacga
     4921 gggggagaac aaggaggagg acagcagtgt gattcattat gacaatgagg ccatcgctcg
     4981 gctgttggac cggaaccagg atgcaactga ggacactgac gtgcagaaca tgaatgagta
     5041 tctcagctcc ttcaaggtgg cacagtacgt cgtgcgggaa gaagacaaga ttgaggaaat
     5101 tgagcgagag atcatcaagc aggaggagaa tgtggaccct gactactggg agaagctgct
     5161 gaggcatcac tatgagcaac agcaggaaga cctagcccgg aatctaggca agggcaagcg
     5221 ggttcgcaag caagttaact acaatgatgc tgctcaggaa gaccaagaca accagtcaga
     5281 gtactcggtg ggttcagagg aggaggatga agacttcgat gaacgtcctg aagggcgtag
     5341 acagtcaaag aggcagctcc ggaatgagaa agataagcca ctgcctccac tgctggcccg
     5401 agtcgggggc aacattgagg tgctgggctt caacacccgt cagcggaagg ctttcctcaa
     5461 tgctgtgatg cgctggggga tgccaccaca ggatgccttc accacacagt ggctggtgcg
     5521 ggacctgagg ggcaagactg agaaggagtt taaggcctat gtgtctttgt tcatgcgcca
     5581 tctgtgtgag cctggggcag acggctctga aacctttgcc gatggggtcc ctcgggaggg
     5641 actgagtcgc cagcaggtgt tgacccgcat tggagtcatg tctctcgtca aaaagaaggt
     5701 gcaggagttt gagcacatca atgggcgttg gtcaatgccg gaactgatgc ctgaccccag
     5761 cgccgattct aagcgctcct ccagagcctc ctctcctacc aaaacgtctc ccaccactcc
     5821 tgaggcttct gctaccaaca gtccctgcac ctctaaacct gctactccag ctccaagtga
     5881 gaaaggagaa ggcataagga cacctcttga gaaggaggaa gctgaaaacc aggaggaaaa
     5941 gccagagaag aacagcagaa ttggggagaa gatggagaca gaggctgatg cccccagccc
     6001 agccccatca cttggggagc ggctggagcc aaggaagatt cctctagagg atgaggtgcc
     6061 aggggtgcct ggagagatgg agcctgaacc tgggtaccgt ggggacagag agaagtcagc
     6121 cacagagtcg acgccaggag aaagggggga ggagaagccg ttggatggac aggaacacag
     6181 ggagaggccg gagggggaaa caggggattt gggcaagaga gaagatgtaa aaggtgaccg
     6241 ggagcttcga ccagggcctc gagatgagcc acggtccaat gggcgacgag aggaaaagac
     6301 agagaagccc cggttcatgt tcaatatcgc cgatggtggc ttcacagagc ttcacacact
     6361 gtggcagaat gaggaacggg cagctatttc ctcggggaaa ctcaatgaga tctggcacag
     6421 aagacatgac tattggcttc tggctgggat tgtcctccat ggctatgcac ggtggcagga
     6481 catccagaat gatgctcaat ttgccattat caacgagcca tttaaaactg aagccaataa
     6541 ggggaacttt ctggagatga aaaataagtt cctggcccgg aggttcaagc tcctggagca
     6601 ggcgctggtg attgaggagc agctgcggcg ggcggcctac ctgaacctgt cgcaggagcc
     6661 ggcgcacccc gccatggccc tccacgcccg cttcgccgag gccgagtgcc tggccgagag
     6721 ccaccagcac ctctccaagg agtcgctggc ggggaacaag ccggccaacg ccgtcctgca
     6781 caaggttctg aaccagctgg aggagttgct gagcgacatg aaggcggacg tgacccgcct
     6841 gccagccacg ctgtcccgaa taccccccat cgcagcccgc cttcagatgt ccgagcgcag
     6901 catcctcagc cggctggcca gcaagggcac ggagcctcac cccacaccgg cctacccgcc
     6961 gggtccctac gctacacctc cggggtacgg ggcggccttc agcgccgcac ccgtaggggc
     7021 cctggccgcc gcaggcgcca attacagcca gatgcctgca gggtccttca tcacagccgc
     7081 caccaacggc cctccagtgc ttgtgaagaa ggagaaggaa atggtggggg cattggtgtc
     7141 agacgggctg gatcggaagg agccccgagc cggggaggtg atctgtatag acgactagta
     7201 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7261 ggacgacacg gccataattg gttcagcgtg acgcgttaag tcgacaatca acctctggat
     7321 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7381 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7441 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7501 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7561 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7621 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7681 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7741 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7801 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7861 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     7921 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7981 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8041 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8101 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8161 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8221 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8281 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8341 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8401 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8581 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8641 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8701 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8761 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8821 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8881 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8941 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9001 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9061 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9121 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9181 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9241 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9301 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9361 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9421 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9481 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9541 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9601 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9661 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9721 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9781 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9841 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9901 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9961 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10021 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10081 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10141 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10201 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10261 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11041 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11101 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11161 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11221 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11281 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11341 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11401 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
    11461 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
    11521 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
    11581 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
    11641 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
    11701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11821 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    11881 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    11941 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    12001 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    12061 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    12121 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    12181 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    12241 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    12301 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    12361 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    12421 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    12481 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    12541 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    12601 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    12661 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    12721 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    12781 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    12841 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    12901 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    12961 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    13021 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    13081 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    13141 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
    13201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
    13261 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    13321 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    13381 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    13441 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    13501 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    13561 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
    13621 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    13681 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    13741 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    13801 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    13861 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    13921 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    13981 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    14041 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    14101 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    14161 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    14221 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    14281 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    14341 cttctaggtc ttgaaaggag tgggaatt
//
